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Title: Detection of nucleic acid sequences by invader-directed cleavage

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5{prime} nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
US Department of Energy, Washington, DC (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
20013254
Application Number:
Int. Cl. C12Q 1/68; C12P 19/34; C07H 21/02; US 6,001,567/A/; 8-682,853; TRN: IM200010%%122
Assignee:
PTO; EDB-00:006676
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 14 Dec 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; NUCLEIC ACIDS; DNA SEQUENCING; DESIGN; DNA SEQUENCERS; CLEAVAGE; NUCLEASES; ENZYME ACTIVITY

Citation Formats

Brow, M A.D., Hall, J S.G., Lyamichev, V, Olive, D M, and Prudent, J R. Detection of nucleic acid sequences by invader-directed cleavage. United States: N. p., 1999. Web.
Brow, M A.D., Hall, J S.G., Lyamichev, V, Olive, D M, & Prudent, J R. Detection of nucleic acid sequences by invader-directed cleavage. United States.
Brow, M A.D., Hall, J S.G., Lyamichev, V, Olive, D M, and Prudent, J R. Tue . "Detection of nucleic acid sequences by invader-directed cleavage". United States.
@article{osti_20013254,
title = {Detection of nucleic acid sequences by invader-directed cleavage},
author = {Brow, M A.D. and Hall, J S.G. and Lyamichev, V and Olive, D M and Prudent, J R},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5{prime} nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {12}
}