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Title: Detection of nucleic acids by multiple sequential invasive cleavages

Abstract

The present invention relates to methods for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

Inventors:
; ; ;
Publication Date:
Research Org.:
US Department of Energy, Washington, DC (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
20013245
Application Number:
Int. Cl. C12Q 1/68; C12P 19/34; US 5,994,069/A/; 8-823,516; TRN: IM200010%%113
Assignee:
PTO; EDB-00:006667
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 30 Nov 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; NUCLEIC ACIDS; DNA SEQUENCING; CLEAVAGE; NUCLEASES; ENZYME ACTIVITY; VIRAL DISEASES; DIAGNOSIS; CHEMICAL COMPOSITION; SEPARATION PROCESSES

Citation Formats

Hall, J G, Lyamichev, V I, Mast, A L, and Brow, M A.D. Detection of nucleic acids by multiple sequential invasive cleavages. United States: N. p., 1999. Web.
Hall, J G, Lyamichev, V I, Mast, A L, & Brow, M A.D. Detection of nucleic acids by multiple sequential invasive cleavages. United States.
Hall, J G, Lyamichev, V I, Mast, A L, and Brow, M A.D. Tue . "Detection of nucleic acids by multiple sequential invasive cleavages". United States.
@article{osti_20013245,
title = {Detection of nucleic acids by multiple sequential invasive cleavages},
author = {Hall, J G and Lyamichev, V I and Mast, A L and Brow, M A.D.},
abstractNote = {The present invention relates to methods for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {11}
}