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Pumping Iron: A Multi-omics Analysis of Two Extremophilic Algae Reveals Iron Economy Management

Journal Article · · Proceedings of the National Academy of Sciences (PNAS)
 [1];  [1];  [1];  [2];  [2];  [2];  [3];  [4];  [5];  [6];  [7];  [7];  [3]
  1. University of California, Los Angeles
  2. BATTELLE (PACIFIC NW LAB)
  3. University of California, Berkeley
  4. Michigan State University
  5. Washington State University
  6. Brookhaven National Laboratory
  7. Purdue University
+ Show Author Affiliations

Marine algae are responsible for half of the world's primary productivity, but this critical carbonsink is often constrained by insufficient iron. One species of marine algae, Dunaliella tertiolecta, isremarkable for its ability to maintain photosynthesis and thrive in low-iron environments. A relatedspecies, Dunaliella salina Bardawil, shares this attribute but is an extremophile found in hypersaline environments. To elucidate how algae manage their iron requirements, we produced highquality genome assemblies and transcriptomes for both species to serve as a foundation for acomparative multi-omics analysis. We identified a host of iron-uptake proteins in both species,including a massive expansion of transferrins and a novel family of siderophore-iron uptakeproteins. Complementing these multiple iron-uptake routes, ferredoxin functions as a large ironreservoir that can be released by induction of flavodoxin. Proteomic analysis revealed reducedinvestment in the photosynthetic apparatus coupled with remodeling of antenna proteins bydramatic iron-deficiency induction of TIDI1, a light harvesting complex protein found also in otherchlorophytes. These combinatorial iron scavenging and sparing strategies make Dunaliellaunique among photosynthetic organisms

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
2000684
Report Number(s):
PNNL-SA-187681
Journal Information:
Proceedings of the National Academy of Sciences (PNAS), Journal Name: Proceedings of the National Academy of Sciences (PNAS) Journal Issue: 30 Vol. 120
Country of Publication:
United States
Language:
English

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MOESM3 of RNA-Seq transcriptomic analysis with Bag2D software identifies key pathways enhancing lipid yield in a high lipid-producing mutant of the non-model green alga Dunaliella tertiolecta dataset January 2015

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Pumping iron: A multi-omics analysis of two extremophilic algae reveals iron economy management
Journal Article · 2023 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:1989950

Related Subjects

iron homeostasis
phytoplankton
Iron starvation induced protein (ISIP)
Chlamydonas reinhardtii
flavin biosynthesis