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Title: Relationship between polychlorinated biphenyl 126 treatment and cytochrome P4501A activity in chickens, as measured by in vivo caffeine and ex vivo ethoxyresorufin metabolism

Abstract

Cytochrome P4501A (CYPIA) activity is often used as a biomarker of exposure of wildlife to polyhalogenated diaromatic hydrocarbons (PHDHs) and is usually measured ex vivo in liver tissue. A caffeine breath test with radiolabeled substrate ({sup 14}C-CBT) has been developed to measure in vivo avian CYPIA activity. Research goals were to develop stable isotope methods ({sup 13}C-CBT), determine dose-response relationships between caffeine N-demethylation (CNDM) and PHDH exposure, and assess the relative utility of the CBT and ex vivo ethoxyresorufin-O-deethylase (EROD) assay. The {sup 13}C-CBT methods were developed with 20 chickens (Gallus domesticus). Chickens received three intraperitoneal injections of 0, 1, 5, or 50 {micro}g 3,3{prime},4,4{prime},5-pentachlorobiphenyl (PCB 126)/kg body weight, and CNDM was quantified by measurement of {sup 13}CO{sub 2}/{sup 12}CO{sub 2} in expired breath. The {sup 13}C-CBT was not as sensitive or specific as the EROD assay as an indicator of PHDH exposure and effect in birds. Constitutive CNDM of great interindividual variability was observed, and the magnitude of induction was greater for EROD activity than for CNDM (approximately 1,000- and 2-fold, respectively). Variability associated with baseline {sup 13}CO{sub 2}/{sup 12}CO{sub 2} ratios in expired breath reduced the sensitivity of the {sup 13}C-CBT method.

Authors:
; ;
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (US)
OSTI Identifier:
20006630
Alternate Identifier(s):
OSTI ID: 20006630
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 18; Journal Issue: 9; Other Information: PBD: Sep 1999; Journal ID: ISSN 0730-7268
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; WATER POLLUTION; POLYCHLORINATED BIPHENYLS; BIOASSAY; HYDROXYLASES; BIOLOGICAL MARKERS; OXIDOREDUCTASES; ENZYME ACTIVITY

Citation Formats

Feyk, L.A., Giesy, J.P., and Lambert, G.H. Relationship between polychlorinated biphenyl 126 treatment and cytochrome P4501A activity in chickens, as measured by in vivo caffeine and ex vivo ethoxyresorufin metabolism. United States: N. p., 1999. Web. doi:10.1897/1551-5028(1999)018<2013:RBPBTA>2.3.CO;2.
Feyk, L.A., Giesy, J.P., & Lambert, G.H. Relationship between polychlorinated biphenyl 126 treatment and cytochrome P4501A activity in chickens, as measured by in vivo caffeine and ex vivo ethoxyresorufin metabolism. United States. doi:10.1897/1551-5028(1999)018<2013:RBPBTA>2.3.CO;2.
Feyk, L.A., Giesy, J.P., and Lambert, G.H. Wed . "Relationship between polychlorinated biphenyl 126 treatment and cytochrome P4501A activity in chickens, as measured by in vivo caffeine and ex vivo ethoxyresorufin metabolism". United States. doi:10.1897/1551-5028(1999)018<2013:RBPBTA>2.3.CO;2.
@article{osti_20006630,
title = {Relationship between polychlorinated biphenyl 126 treatment and cytochrome P4501A activity in chickens, as measured by in vivo caffeine and ex vivo ethoxyresorufin metabolism},
author = {Feyk, L.A. and Giesy, J.P. and Lambert, G.H.},
abstractNote = {Cytochrome P4501A (CYPIA) activity is often used as a biomarker of exposure of wildlife to polyhalogenated diaromatic hydrocarbons (PHDHs) and is usually measured ex vivo in liver tissue. A caffeine breath test with radiolabeled substrate ({sup 14}C-CBT) has been developed to measure in vivo avian CYPIA activity. Research goals were to develop stable isotope methods ({sup 13}C-CBT), determine dose-response relationships between caffeine N-demethylation (CNDM) and PHDH exposure, and assess the relative utility of the CBT and ex vivo ethoxyresorufin-O-deethylase (EROD) assay. The {sup 13}C-CBT methods were developed with 20 chickens (Gallus domesticus). Chickens received three intraperitoneal injections of 0, 1, 5, or 50 {micro}g 3,3{prime},4,4{prime},5-pentachlorobiphenyl (PCB 126)/kg body weight, and CNDM was quantified by measurement of {sup 13}CO{sub 2}/{sup 12}CO{sub 2} in expired breath. The {sup 13}C-CBT was not as sensitive or specific as the EROD assay as an indicator of PHDH exposure and effect in birds. Constitutive CNDM of great interindividual variability was observed, and the magnitude of induction was greater for EROD activity than for CNDM (approximately 1,000- and 2-fold, respectively). Variability associated with baseline {sup 13}CO{sub 2}/{sup 12}CO{sub 2} ratios in expired breath reduced the sensitivity of the {sup 13}C-CBT method.},
doi = {10.1897/1551-5028(1999)018<2013:RBPBTA>2.3.CO;2},
journal = {Environmental Toxicology and Chemistry},
issn = {0730-7268},
number = 9,
volume = 18,
place = {United States},
year = {1999},
month = {9}
}