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Title: Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast

Abstract

For many organisms there is a correlation between increases of metabolites and osmotic stress tolerance, but the mechanisms that cause this protection are not clear. To understand the role of polyols, genes for bacterial mannitol-1-P dehydrogenase and apply sorbitol-6-P dehydrogenase were introduced into a Saccharomyces cerevisiae mutant deficient in glycerol synthesis. Sorbitol and mannitol provided some protection, but less than that generated by a similar concentration of glycerol generated by glycerol-3-P dehydrogenase (GPD1). Reduced protection by polyols suggested that glycerol had specific functions for which mannitol and sorbitol could not substitute, and that the absolute amount of the accumulating osmoticum might not be crucial. The retention of glycerol and mannitol-sorbitol, respectively, was a major difference. During salt stress, cells retained more of the six-carbon polyois than glycerol. The authors suggest that the loss of {gt} 98% of the glycerol synthesized could provide a safety value that dissipates reducing power, which a similar high intracellular concentration of retained polyois would be less protective. To understand the role of glycerol in salt tolerance, salt-tolerant suppressor mutants were isolated from the glycerol-deficient strain. One mutant, sr13, partially suppressed the salt-sensitive phenotype of the glycerol-deficient line, probably due to a doubling of [K{sup +}]more » accumulating during stress. The authors compare these results to the osmotic adjustment concept typically applied to accumulating metabolites in plants. The accumulation of polyois may have dual functions: facilitating osmotic adjustment and supporting redox control.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Arizona, Tucson, AZ (US)
OSTI Identifier:
20006204
Resource Type:
Journal Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 121; Journal Issue: 1; Other Information: PBD: Sep 1999; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; SACCHAROMYCES CEREVISIAE; BIOLOGICAL ADAPTATION; GLYCEROL; BIOSYNTHESIS; OSMOSIS; SODIUM CHLORIDES; BIOLOGICAL STRESS; BIOLOGICAL PATHWAYS; METABOLISM

Citation Formats

Shen, B., Hohmann, S., Jensen, R.G., and Bohnert, H.J. Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast. United States: N. p., 1999. Web. doi:10.1104/pp.121.1.45.
Shen, B., Hohmann, S., Jensen, R.G., & Bohnert, H.J. Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast. United States. doi:10.1104/pp.121.1.45.
Shen, B., Hohmann, S., Jensen, R.G., and Bohnert, H.J. Wed . "Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast". United States. doi:10.1104/pp.121.1.45.
@article{osti_20006204,
title = {Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast},
author = {Shen, B. and Hohmann, S. and Jensen, R.G. and Bohnert, H.J.},
abstractNote = {For many organisms there is a correlation between increases of metabolites and osmotic stress tolerance, but the mechanisms that cause this protection are not clear. To understand the role of polyols, genes for bacterial mannitol-1-P dehydrogenase and apply sorbitol-6-P dehydrogenase were introduced into a Saccharomyces cerevisiae mutant deficient in glycerol synthesis. Sorbitol and mannitol provided some protection, but less than that generated by a similar concentration of glycerol generated by glycerol-3-P dehydrogenase (GPD1). Reduced protection by polyols suggested that glycerol had specific functions for which mannitol and sorbitol could not substitute, and that the absolute amount of the accumulating osmoticum might not be crucial. The retention of glycerol and mannitol-sorbitol, respectively, was a major difference. During salt stress, cells retained more of the six-carbon polyois than glycerol. The authors suggest that the loss of {gt} 98% of the glycerol synthesized could provide a safety value that dissipates reducing power, which a similar high intracellular concentration of retained polyois would be less protective. To understand the role of glycerol in salt tolerance, salt-tolerant suppressor mutants were isolated from the glycerol-deficient strain. One mutant, sr13, partially suppressed the salt-sensitive phenotype of the glycerol-deficient line, probably due to a doubling of [K{sup +}] accumulating during stress. The authors compare these results to the osmotic adjustment concept typically applied to accumulating metabolites in plants. The accumulation of polyois may have dual functions: facilitating osmotic adjustment and supporting redox control.},
doi = {10.1104/pp.121.1.45},
journal = {Plant Physiology (Bethesda)},
issn = {0032-0889},
number = 1,
volume = 121,
place = {United States},
year = {1999},
month = {9}
}