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Title: Radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils

Abstract

Microorganisms that oxidize atmospheric methane in soils were characterized by radioactive labeling with {sup 14}CH{sub 4} followed by analysis of radiolabelled phospholipid ester-linked fatty acids ({sup 14}C-PLFAs). The radioactive fingerprinting technique was used to compare active methanotrophs in soil samples from Greenland, Denmark, the United States, and Brazil. The {sup 14}C-PLFA fingerprints indicated that closely related methanotrophic bacteria were responsible for the oxidation of atmospheric methane in the soils. Significant amounts of labeled PLFAs produced by the unknown soil methanotrophs coeluted with a group of fatty acids that included i17:0, a17:0, and 17:1{omega}8c. These PLFAs are not known to be significant constituents of methanotrophic bacteria. The major PLFAs of the soil methanotrophs coeluted with 18:1 and 18:0 fatty acids. The {sup 14}C-PLFAs fingerprints of the soil methanotrophs that oxidized atmospheric methane did not change after long-term methane enrichment at 170 ppm CH{sub 4}. The {sup 14}C-PLFA fingerprints of the soil methanotrophs were different from the PLFA profiles of type 1 and type 2 methanotrophic bacteria described previously. Some similarity at the PLFA level was observed between the unknown soil methanotrophs and the PLFA phenotype of the type 2 methanotrophs.

Authors:
;
Publication Date:
Research Org.:
Aalborg Univ. (DK)
OSTI Identifier:
20000241
Alternate Identifier(s):
OSTI ID: 20000241
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 65; Journal Issue: 9; Other Information: PBD: Sep 1999; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; SOILS; METHANE; BIODEGRADATION; CARBON 14 COMPOUNDS; LABELLED COMPOUNDS; METHANOTROPHIC BACTERIA

Citation Formats

Roslev, P., and Iversen, N. Radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils. United States: N. p., 1999. Web.
Roslev, P., & Iversen, N. Radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils. United States.
Roslev, P., and Iversen, N. Wed . "Radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils". United States.
@article{osti_20000241,
title = {Radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils},
author = {Roslev, P. and Iversen, N.},
abstractNote = {Microorganisms that oxidize atmospheric methane in soils were characterized by radioactive labeling with {sup 14}CH{sub 4} followed by analysis of radiolabelled phospholipid ester-linked fatty acids ({sup 14}C-PLFAs). The radioactive fingerprinting technique was used to compare active methanotrophs in soil samples from Greenland, Denmark, the United States, and Brazil. The {sup 14}C-PLFA fingerprints indicated that closely related methanotrophic bacteria were responsible for the oxidation of atmospheric methane in the soils. Significant amounts of labeled PLFAs produced by the unknown soil methanotrophs coeluted with a group of fatty acids that included i17:0, a17:0, and 17:1{omega}8c. These PLFAs are not known to be significant constituents of methanotrophic bacteria. The major PLFAs of the soil methanotrophs coeluted with 18:1 and 18:0 fatty acids. The {sup 14}C-PLFAs fingerprints of the soil methanotrophs that oxidized atmospheric methane did not change after long-term methane enrichment at 170 ppm CH{sub 4}. The {sup 14}C-PLFA fingerprints of the soil methanotrophs were different from the PLFA profiles of type 1 and type 2 methanotrophic bacteria described previously. Some similarity at the PLFA level was observed between the unknown soil methanotrophs and the PLFA phenotype of the type 2 methanotrophs.},
doi = {},
journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 9,
volume = 65,
place = {United States},
year = {1999},
month = {9}
}