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Title: Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology

Abstract

Measurements of the hydrogen consumption threshold and the tracking of electrons transferred to the chlorinated electron acceptor (f{sub e}) reliably detected chlororespiratory physiology in both mixed cultures and pure cultures capable of using tetrachloroethene, cis-1,2-dichloroethene, vinyl chloride, 2-chlorophenol, 3-chlorobenzoate, 3-chloro-4-hydroxybenzoate, or 1,2-dichloropropane as an electron acceptor. Hydrogen was consumed to significantly lower threshold concentrations of less than 0.4 ppmv compared with the values obtained for the same cultures without a chlorinated compound as an electron acceptor. The f{sub e} values ranged from 0.63 to 0.7, values which are in good agreement with theoretical calculations based on the thermodynamics of reductive dechlorination as the terminal electron-accepting process. In contrast, a mixed methanogenic culture that cometabolized 3-chlorophenol exhibited a significantly lower f{sub e} value, 0.012.

Authors:
; ;
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (US)
Sponsoring Org.:
Alexander von Humboldt Foundation; USDOE; National Science Foundation (NSF)
OSTI Identifier:
20000240
DOE Contract Number:  
FG07-96ER62319
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 65; Journal Issue: 9; Other Information: PBD: Sep 1999; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; REMEDIAL ACTION; ELECTRON TRANSFER; CHLORINATED ALIPHATIC HYDROCARBONS

Citation Formats

Loeffler, F.E., Tiedje, J.M., and Sanford, R.A. Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology. United States: N. p., 1999. Web.
Loeffler, F.E., Tiedje, J.M., & Sanford, R.A. Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology. United States.
Loeffler, F.E., Tiedje, J.M., and Sanford, R.A. Wed . "Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology". United States.
@article{osti_20000240,
title = {Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology},
author = {Loeffler, F.E. and Tiedje, J.M. and Sanford, R.A.},
abstractNote = {Measurements of the hydrogen consumption threshold and the tracking of electrons transferred to the chlorinated electron acceptor (f{sub e}) reliably detected chlororespiratory physiology in both mixed cultures and pure cultures capable of using tetrachloroethene, cis-1,2-dichloroethene, vinyl chloride, 2-chlorophenol, 3-chlorobenzoate, 3-chloro-4-hydroxybenzoate, or 1,2-dichloropropane as an electron acceptor. Hydrogen was consumed to significantly lower threshold concentrations of less than 0.4 ppmv compared with the values obtained for the same cultures without a chlorinated compound as an electron acceptor. The f{sub e} values ranged from 0.63 to 0.7, values which are in good agreement with theoretical calculations based on the thermodynamics of reductive dechlorination as the terminal electron-accepting process. In contrast, a mixed methanogenic culture that cometabolized 3-chlorophenol exhibited a significantly lower f{sub e} value, 0.012.},
doi = {},
journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 9,
volume = 65,
place = {United States},
year = {1999},
month = {9}
}