Spectral Deconvolution of Electron-Bifurcating Flavoproteins
Electron-bifurcating flavoproteins catalyze the tightly coupled reduction of high- and low-potential acceptors using a median-potential electron donor, and are invariably complex systems with multiple redox-active centers in two or more subunits. Methods are described that permit, in favorable cases, the deconvolution of spectral changes associated with reduction of specific centers, making it possible to dissect the overall process of electron bifurcation into individual, discrete steps.
- Research Organization:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1984208
- Report Number(s):
- NREL/CH-2700-86258; MainId:87031; UUID:ac80e10c-200e-4ea6-a076-75bf206c7f0a; MainAdminID:69491
- Country of Publication:
- United States
- Language:
- English
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Journal Article
·
2020
· Proceedings of the National Academy of Sciences of the United States of America
·
OSTI ID:1749975
+2 more