Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

An Analysis of Postharvest Fungal Pathogens Reveals Temporal–Spatial and Host–Pathogen Associations with Fungicide Resistance-Related Mutations

Journal Article · · Phytopathology
 [1];  [1];  [2];  [2];  [1]
  1. Food Quality Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705
  2. School of Environmental and Forest Sciences, University of Washington, Seattle, WA 98195
+ Show Author Affiliations

Fungicides are the primary tools to control a wide range of postharvest fungal pathogens. Fungicide resistance is a widespread problem that has reduced the efficacy of fungicides. Resistance to FRAC-1 (Fungicide Resistance Action Committee-1) chemistries is associated with mutations in amino acid position 198 in the β-tubulin gene. In our study, we conducted a meta-analysis of β-tubulin sequences to infer temporal, spatial, plant host, and pathogen genus patterns of fungicide resistance in postharvest fungal pathogens. In total, data were acquired from 2,647 specimens from 12 genera of fungal phytopathogens residing in 53 countries on >200 hosts collected between 1926 and 2020. The specimens containing a position 198 mutation were globally distributed in a variety of pathosystems. Analyses showed that there are associations among the mutation and the year an isolate was collected, the pathogen genus, the pathogen host, and the collection region. Interestingly, fungicide-resistant β-tubulin genotypes have been in a decline since their peak between 2005 and 2009. FRAC-1 fungicide usage data followed a similar pattern in that applications have been in a decline since their peak between 1997 and 2003. The data show that, with the reduction of selection pressure, FRAC-1 fungicide resistance in fungal populations will decline within 5 to 10 years. Based on this line of evidence, we contend that a β-tubulin position 198 mutation has uncharacterized fitness cost(s) on fungi in nature. The compiled dataset can inform end users on the regions and hosts that are most prone to contain resistant pathogens and assist decisions concerning fungicide resistance management strategies.

Research Organization:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
SC0014664
OSTI ID:
1982731
Journal Information:
Phytopathology, Journal Name: Phytopathology Journal Issue: 11 Vol. 111; ISSN 0031-949X
Publisher:
American Phytopathological Society
Country of Publication:
United States
Language:
English

References (40)

The Power of Meta-Analysis in Genome-Wide Association Studies journal August 2013
Identification of a novel point mutation in the β-tubulin gene of Botrytis cinerea and detection of benzimidazole resistance by a diagnostic PCR-RFLP assay journal March 2009
Detection and Molecular Characterization of Resistance to the Dicarboximide and Benzamide Fungicides in Botrytis cinerea From Tomato in Hubei Province, China journal July 2018
PIRA-PCR for detection ofFusarium graminearumgenotypes with moderate resistance to carbendazim journal October 2009
Measuring IPM Impacts in California and Arizona journal January 2016
Identification and Characterization of Benomyl-Resistant and -Sensitive Populations of Colletotrichum gloeosporioides from Statice (Limonium spp.) journal May 2006
Two Mutations in β-Tubulin 2 Gene Associated with Thiophanate-Methyl Resistance in Colletotrichum cereale Isolates from Creeping Bentgrass in Mississippi and Alabama journal February 2010
Mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of Penicillium digitatum collected from citrus in Taiwan journal November 2011
Mutations Leading to Substitutions at Amino Acids 198 and 200 of Beta-Tubulin that Correlate with Benomyl-Resistance Phenotypes of Field Strains ofBotrytis cinerea journal January 1993
Identification and Characterization of BenzimidazoleResistance in Monilinia fructicola from Stone Fruit OrchardsinCalifornia journal December 2003
Meta-Analysis of Genetic Association Studies journal May 2015
Rapid identification of benomyl resistant strains of Botrytis cinerea using the polymerase chain reaction journal December 1995
Population genetics-informed meta-analysis in seven genes associated with risk to dengue fever disease journal August 2018
Antimitotic activity of methyl benzimidazol-2-yl carbamate (MBC) in Aspergillus nidulans journal September 1973
Fungicide Sensitivity and Phylogenetic Relationship of Anthracnose Fungi Isolated from Various Fruit Crops in Japan journal April 2006
The Problem of Fungicide Resistance journal November 1969
Mutational Resistance to Fungitoxic Benzimidazole Derivatives in Aspergillus nidulans journal August 1971
First Estimation of the Spontaneous Mutation Rate in Diatoms journal June 2019
Benzimidazole and diphenylamine sensitivity and identity of Penicillium spp. that cause postharvest blue mold of apples using β-tubulin gene sequences journal April 2005
Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of β-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1 journal December 2007
Meta-analysis of genetic association studies journal September 2004
Frequency of Benzimidazole- and Dicarboximide-Resistant Strainsof Botrytis cinereain Western Oregon Small Fruit and Snap Bean Plantings journal January 1994
Detection and Molecular Characterization of Benzimidazole Resistance Among Colletotrichum truncatum Isolates Infecting Bell Pepper in Trinidad journal June 2016
Amino acid alterations in thebenA (β-tubulin) gene ofAspergillus nidulans that confer benomyl resistance journal January 1992
Incidence of postharvest pathogens of stored apples in British Columbia journal March 1996
M233I Mutation in the β-Tubulin of Botrytis cinerea Confers Resistance to Zoxamide journal November 2015
Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP journal February 2010
Characterization of Mutations in the Beta-Tubulin Gene of Benomyl-Resistant Field Strains ofVenturia inaequalisand Other Plant Pathogenic Fungi journal January 1992
Detection of resistance in Sclerotinia sclerotiorum to carbendazim and dimethachlon in Jiangsu Province of China journal February 2014
Within-Season Shift in Fungicide Resistance Profiles of Botrytis cinerea in California Strawberry Fields journal January 2019
Genotyping of Benzimidazole-Resistant and Dicarboximide-Resistant Mutations in Botrytis cinerea Using Real-Time Polymerase Chain Reaction Assays journal April 2008
Thiabendazole resistance and mutations in the β-tubulin gene ofPenicillium expansumstrains isolated from apples and pears with blue mold decay journal August 2009
First Report of Colletotrichum musicola Causing Soybean Anthracnose in Brazil journal June 2020
Identification of an amino acid substitution in thebenA, ?-tubulin gene ofAspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity journal January 1990
Bitter Rot of Apple in the Mid-Atlantic United States: Causal Species and Evaluation of the Impacts of Regional Weather Patterns and Cultivar Susceptibility journal June 2021
Identification and Characterization of Fungicide Resistance in Botrytis Populations from Small Fruit Fields in the Mid-Atlantic United States journal September 2021
Mutations in a β-Tubulin Confer Resistance of Gibberella zeae to Benzimidazole Fungicides journal December 2009
Isolation, characterization, and expression of a second beta-tubulin-encoding gene from Colletotrichum gloeosporioides f. sp. aeschynomene journal November 1994
Studies on thiabendazole resistance of Penicillium expansum of pears: pathogenic fitness and genetic characterization journal June 2003
Field Strains of Monilinia fructicola Resistant to Both MBC and DMI Fungicides Isolated from Stone Fruit Orchards in the Eastern United States journal August 2013

Similar Records

Nanosulfur: A Potent Fungicide Against Food Pathogen, Aspergillus niger
Journal Article · 2010 · AIP Conference Proceedings · OSTI ID:21457018
Altered patterns of gene duplication and differential gene gain and loss in fungal pathogens
Journal Article · 2008 · BMC Genomics · OSTI ID:1626461

Related Subjects

Plant Sciences