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Atomic-Resolution Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain

Journal Article · · Journal of the American Chemical Society
DOI:https://doi.org/10.1021/jacs.2c03320· OSTI ID:1982062
 [1];  [1];  [1];  [2];  [3];  [2];  [2];  [4];  [4];  [5];  [5]
  1. Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, United States
  2. Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, United States
  3. Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, United States
  4. Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, United States; Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, United States
  5. Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, United States; Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, United States
+ Show Author Affiliations

Not provided.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-76SF00515
OSTI ID:
1982062
Journal Information:
Journal of the American Chemical Society, Vol. 144, Issue 23; ISSN 0002-7863
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English

References (48)

The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2 journal March 2020
A critical analysis of the impacts of COVID-19 on the global economy and ecosystems and opportunities for circular economy strategies journal January 2021
The socio-economic implications of the coronavirus pandemic (COVID-19): A review journal June 2020
Origin and evolution of pathogenic coronaviruses journal December 2018
Mechanisms of Antiviral Immune Evasion of SARS-CoV-2 journal March 2022
The Coronavirus Nucleocapsid Is a Multifunctional Protein journal August 2014
Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates journal November 2020
Modular organization of SARS coronavirus nucleocapsid protein journal October 2005
Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein journal November 2012
Crystal structure-based exploration of the important role of Arg106 in the RNA-binding domain of human coronavirus OC43 nucleocapsid protein journal June 2013
Transient Oligomerization of the SARS-CoV N Protein – Implication for Virus Ribonucleoprotein Packaging journal May 2013
The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA journal March 2021
The SARS coronavirus nucleocapsid protein – Forms and functions journal March 2014
Structures of the SARS ‐CoV‐2 nucleocapsid and their perspectives for drug design journal September 2020
Structural Insight Into the SARS-CoV-2 Nucleocapsid Protein C-Terminal Domain Reveals a Novel Recognition Mechanism for Viral Transcriptional Regulatory Sequences journal January 2021
The highly flexible disordered regions of the SARS-CoV-2 nucleocapsid N protein within the 1–248 residue construct: sequence-specific resonance assignments through NMR journal March 2021
Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites journal April 2020
Coronavirus N Protein N-Terminal Domain (NTD) Specifically Binds the Transcriptional Regulatory Sequence (TRS) and Melts TRS-cTRS RNA Duplexes journal December 2009
Structure of the SARS Coronavirus Nucleocapsid Protein RNA-binding Dimerization Domain Suggests a Mechanism for Helical Packaging of Viral RNA journal May 2007
Structure of the N-Terminal RNA-Binding Domain of the SARS CoV Nucleocapsid Protein journal May 2004
Ribonucleocapsid Formation of Severe Acute Respiratory Syndrome Coronavirus through Molecular Action of the N-Terminal Domain of N Protein journal January 2007
Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein journal December 2020
Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection journal March 2021
Broadband homonuclear correlation spectroscopy driven by combined R2nv sequences under fast magic angle spinning for NMR structural analysis of organic and biological solids journal July 2013
Letter to the Editor: 1H, 13C, and 15N resonance assignments of the N-terminal domain of the SARS CoV nucleocapsid protein journal January 2005
Accuracy and precision of protein structures determined by magic angle spinning NMR spectroscopy: for some ‘with a little help from a friend’ journal March 2019
A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation journal May 2021
Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies journal July 2021
Solid-State 15 N NMR Chemical Shift Anisotropy of Histidines:  Experimental and Theoretical Studies of Hydrogen Bonding † journal November 1999
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR journal February 2011
Tautomeric states of the active-site histidines of phosphorylated and unphosphorylated IIIGlc, a signal-transducing protein from escherichia coli, using two-dimensional heteronuclear NMR techniques journal April 1993
The Structure of the Cataract-Causing P23T Mutant of Human γD-Crystallin Exhibits Distinctive Local Conformational and Dynamic Changes, journal February 2009
Hydrogen-Bonding Partner of the Proton-Conducting Histidine in the Influenza M2 Proton Channel Revealed From 1H Chemical Shifts journal August 2012
XDS journal January 2010
Cross polarization in the tilted frame: assignment and spectral simplification in heteronuclear spin systems journal December 1998
Improved Proton Decoupling in NMR Spectroscopy of Crystalline Solids Using the S PINAL -64 Sequence journal December 2002
Anisotropic Collective Motion Contributes to Nuclear Spin Relaxation in Crystalline Proteins journal February 2010
Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning journal August 2014
NMRPipe: A multidimensional spectral processing system based on UNIX pipes journal November 1995
A software framework for analysing solid-state MAS NMR data journal September 2011
NMRFAM-SPARKY: enhanced software for biomolecular NMR spectroscopy journal December 2014
Using Xplor–NIH for NMR molecular structure determination journal March 2006
Xplor-NIH for molecular structure determination from NMR and other data sources: Xplor-NIH for Molecular Structure Determination journal September 2017
Protein Structural Information Derived from NMR Chemical Shift with the Neural Network Program TALOS-N book November 2014
Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR journal September 2020
A practical implicit solvent potential for NMR structure calculation journal June 2014
A three‐dimensional potential of mean force to improve backbone and sidechain hydrogen bond geometry in Xplor‐NIH protein structure determination journal October 2019
STRIDE: a web server for secondary structure assignment from known atomic coordinates of proteins journal July 2004

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