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Computationally Guided Redesign of a Heme-free Cytochrome with Native-like Structure and Stability

Journal Article · · Biochemistry

Not provided.

Research Organization:
Univ. of California, San Diego, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
SC0003844
OSTI ID:
1977766
Journal Information:
Biochemistry, Vol. 61, Issue 19; ISSN 0006-2960
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English

References (57)

Principles of Protein Stability and Their Application in Computational Design journal June 2018
Metal-mediated protein stabilization journal May 1994
Structural and Functional Aspects of Metal Sites in Biology journal January 1996
Design of functional metalloproteins journal August 2009
Role of Cofactors in Protein Folding journal February 2002
Metal-dependent folding of a single zinc finger from transcription factor IIIA. journal July 1987
Zinc-Dependent Structure of a Single-Finger Domain of Yeast ADR1 journal September 1988
Structure of human oxyhaemoglobin at 2·1resolution journal November 1983
Mechanism of Oxidation Reactions Catalyzed by Cytochrome P450 Enzymes journal September 2004
The role of cytochrome P450 enzymes in endogenous signalling pathways and environmental carcinogenesis journal December 2006
Effect of heme binding on the structure and stability of Escherichia coli apocytochrome b562 journal October 1991
Solution structure of apocytochrome b562 journal January 1994
Metallation and mismetallation of iron and manganese proteins in vitro and in vivo: the class I ribonucleotide reductases as a case study journal January 2012
Protein-folding location can regulate manganese-binding versus copper- or zinc-binding journal October 2008
The Interaction of Mitochondrial Iron with Manganese Superoxide Dismutase journal August 2009
Assembly of Nitrogenase MoFe Protein journal April 2008
Maturation of Iron-Sulfur Proteins in Eukaryotes: Mechanisms, Connected Processes, and Diseases journal June 2008
Cytochrome c biogenesis: the Ccm system journal June 2010
Characterization and crystal structure of zinc azurin, a by-product of heterologous expression in Escherichia coli of Pseudomonas aeruginosa copper azurin journal May 1992
Importance of the structural zinc atom for the stability of yeast alcohol dehydrogenase journal October 1992
Bacterial and Archaeal α-Amylases: Diversity and Amelioration of the Desirable Characteristics for Industrial Applications journal July 2016
Design of a Monomeric 23-Residue Polypeptide with Defined Tertiary Structure journal January 1996
De Novo Protein Design: Fully Automated Sequence Selection journal October 1997
A Large Scale Test of Computational Protein Design: Folding and Stability of Nine Completely Redesigned Globular Proteins journal September 2003
High-resolution Structural and Thermodynamic Analysis of Extreme Stabilization of Human Procarboxypeptidase by Computational Protein Design journal March 2007
Automated selection of stabilizing mutations in designed and natural proteins journal January 2012
Computational Thermostabilization of an Enzyme journal May 2005
Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability journal July 2016
Redesign of a Four-helix Bundle Protein by Phage Display Coupled with Proteolysis and Structural Characterization by NMR and X-ray Crystallography journal October 2002
Rapid Formation of a Four-Helix Bundle. Cytochrome b562 Folding Triggered by Electron Transfer journal October 1997
Cytochrome b 562 folding triggered by electron transfer: Approaching the speed limit for formation of a four-helix-bundle protein journal June 1999
Cytochrome b562 Variants:  A Library for Examining Redox Potential Evolution journal April 2000
Effects of Heme on the Structure of the Denatured State and Folding Kinetics of Cytochrome b562 journal February 2005
Stability and Folding Kinetics of Structurally Characterized Cytochrome c - b 562 , journal September 2006
Metal-Directed Protein Self-Assembly journal May 2010
Controlling Protein−Protein Interactions through Metal Coordination:  Assembly of a 16-Helix Bundle Protein journal November 2007
Control of Protein Oligomerization Symmetry by Metal Coordination: C 2 and C 3 Symmetrical Assemblies through Cu II and Ni II Coordination journal April 2009
An efficient, step-economical strategy for the design of functional metalloproteins journal February 2019
Constructing protein polyhedra via orthogonal chemical interactions journal January 2020
Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays journal March 2012
Designed, Helical Protein Nanotubes with Variable Diameters from a Single Building Block journal August 2015
In Vitro and Cellular Self-Assembly of a Zn-Binding Protein Cryptand via Templated Disulfide Bonds journal August 2013
Overcoming universal restrictions on metal selectivity by protein design journal March 2022
De Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds journal September 2016
Determining the Structural and Energetic Basis of Allostery in a De Novo Designed Metalloprotein Assembly journal July 2018
A designed supramolecular protein assembly with in vivo enzymatic activity journal December 2014
Metal‐Templated Design of Chemically Switchable Protein Assemblies with High‐Affinity Coordination Sites journal September 2020
Metal templated design of protein interfaces journal December 2009
PyRosetta: a script-based interface for implementing molecular modeling algorithms using Rosetta journal January 2010
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol journal January 2008
Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism journal December 2006
Structure of trypanothione reductase from Crithidia fasciculata at 2.6 Å resolution; enzyme–NADP interactions at 2.8 Å resolution journal March 1994
Phaser crystallographic software journal July 2007
Features and development of Coot journal March 2010
Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network journal January 1993
DichroWeb , a website for calculating protein secondary structure from circular dichroism spectroscopic data journal July 2021
Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding journal October 1995