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13 C-Isotope-Assisted Assessment of Metabolic Quenching During Sample Collection from Suspension Cell Cultures

Journal Article · · Analytical Chemistry
 [1];  [2]
  1. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37235, United States
  2. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37235, United States; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37235, United States

Not provided.

Research Organization:
J. Craig Venter Institute, Inc., Rockville, MD (United States); Johns Hopkins Univ., Baltimore, MD (United States); Vanderbilt Univ., Nashville, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
SC0018344; SC0019388; SC0019404; SC0022207
OSTI ID:
1977759
Journal Information:
Analytical Chemistry, Vol. 94, Issue 22; ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English

References (24)

Metabolomics and Isotope Tracing journal May 2018
Application of isotope labeling experiments and 13C flux analysis to enable rational pathway engineering journal December 2015
Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis journal November 2011
Effective Quenching Processes for Physiologically Valid Metabolite Profiling of Suspension Cultured Mammalian Cells journal December 2008
Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis journal November 2017
Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocol journal September 2010
Isotopically nonstationary 13C flux analysis of cyanobacterial isobutyraldehyde production journal July 2017
Evaluation of quenching methods for metabolite recovery in photoautotrophic Synechococcus sp. PCC 7002 journal May 2020
An LC–MS-Based Chemical and Analytical Method for Targeted Metabolite Quantification in the Model Cyanobacterium Synechococcus sp. PCC 7002 journal May 2011
SWATH Tandem Mass Spectrometry Workflow for Quantification of Mass Isotopologue Distribution of Intracellular Metabolites and Fragments Labeled with Isotopic 13 C Carbon journal April 2018
Sampling for Metabolome Analysis of Microorganisms journal May 2007
Evaluation of cell damage caused by cold sampling and quenching for metabolome analysis journal June 2011
Evaluation and Optimization of Metabolome Sample Preparation Methods for Saccharomyces cerevisiae journal January 2013
Quenching for Microalgal Metabolomics: A Case Study on the Unicellular Eukaryotic Green Alga Chlamydomonas reinhardtii journal October 2018
Influence of washing and quenching in profiling the metabolome of adherent mammalian cells: a case study with the metastatic breast cancer cell line MDA-MB-231 journal January 2017
Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum journal August 2007
Leakage-free rapid quenching technique for yeast metabolomics journal June 2008
A guanidine-degrading enzyme controls genomic stability of ethylene-producing cyanobacteria journal August 2021
Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry book October 2013
Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942 journal July 2020
Metabolic flux analysis at ultra short time scale: Isotopically non-stationary 13C labeling experiments journal April 2007
Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria journal April 2004
Fast sampling for quantitative microbial metabolomics: new aspects on cold methanol quenching: metabolite co-precipitation journal July 2014
A guide to metabolic flux analysis in metabolic engineering: Methods, tools and applications journal January 2021