CasZ compositions and methods of use

Doudna, Jennifer A.; Burstein, David; Chen, Janice S.; Harrington, Lucas B.; Paez-Espino, David; Banfield, Jillian F.

CasZ compositions and methods of use

Patent · 13 September 2022

OSTI ID:1925094

Doudna, Jennifer A.; Burstein, David; Chen, Janice S.; Harrington, Lucas B.; Paez-Espino, David; Banfield, Jillian F.

Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,441,137

Application Number:: 16/884,766

OSTI ID:: 1925094

Country of Publication:: United States

Language:: English

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