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Methods for detecting and determining levels of monolignol ester conjugates incorporated into lignin and compounds relating thereto

Patent ·
OSTI ID:1924983
; ; ;

Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.

Research Organization:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC02-07ER64494
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Number(s):
11,414,372
Application Number:
16/252,915
OSTI ID:
1924983
Country of Publication:
United States
Language:
English

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