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Title: Chemical analysis of individual mammalian cells

Abstract

The extremely small size of mammalian cells creates an unusual challenge for the analytical chemist, both in terms of separation and detection. Under a microscope, it is possible to confirm the injection of individual cells such as erythrocyte into capillaries with 10-{mu}m i.d. by hydrostatic pressure. The ionic contents can then be separated by capillary electrophoresis after the cell lyses. Enzymes at the zeptomole level can be monitored by on-column fluorescence enzyme assay. On-column particle-counting immunoassay can be applied to a broad range of analytes (antigens), also at the zeptomole level. The authors report here the simultaneous determination of the amounts of glucose-6-phosphate dehydrogenase (G6PDH) and their activities in individual erythrocytes by using a combination of the two detection schemes. Insights into the degradation of proteins as a function of cell age can be derived.

Authors:
;  [1]
  1. Ames Lab., IA (United States)
Publication Date:
OSTI Identifier:
191665
Report Number(s):
CONF-941098-
TRN: 95:006733-0021
Resource Type:
Conference
Resource Relation:
Conference: FACSS XXI: 21st annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), St. Louis, MO (United States), 2-7 Oct 1994; Other Information: PBD: 1994; Related Information: Is Part Of 21st annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies; PB: 257 p.
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; 40 CHEMISTRY; ANIMAL CELLS; CHEMICAL ANALYSIS; ENZYMES; QUANTITATIVE CHEMICAL ANALYSIS; FLUORESCENCE SPECTROSCOPY; ERYTHROCYTES; ELECTROPHORESIS

Citation Formats

Tan, W., and Yeung, E.S. Chemical analysis of individual mammalian cells. United States: N. p., 1994. Web.
Tan, W., & Yeung, E.S. Chemical analysis of individual mammalian cells. United States.
Tan, W., and Yeung, E.S. Sat . "Chemical analysis of individual mammalian cells". United States. doi:.
@article{osti_191665,
title = {Chemical analysis of individual mammalian cells},
author = {Tan, W. and Yeung, E.S.},
abstractNote = {The extremely small size of mammalian cells creates an unusual challenge for the analytical chemist, both in terms of separation and detection. Under a microscope, it is possible to confirm the injection of individual cells such as erythrocyte into capillaries with 10-{mu}m i.d. by hydrostatic pressure. The ionic contents can then be separated by capillary electrophoresis after the cell lyses. Enzymes at the zeptomole level can be monitored by on-column fluorescence enzyme assay. On-column particle-counting immunoassay can be applied to a broad range of analytes (antigens), also at the zeptomole level. The authors report here the simultaneous determination of the amounts of glucose-6-phosphate dehydrogenase (G6PDH) and their activities in individual erythrocytes by using a combination of the two detection schemes. Insights into the degradation of proteins as a function of cell age can be derived.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Dec 31 00:00:00 EST 1994},
month = {Sat Dec 31 00:00:00 EST 1994}
}

Conference:
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