A centimeter-long bacterium with DNA contained in metabolically active, membrane-bound organelles.

Journal Article · 01 June 2022 · Science (New York, N.Y.)

DOI:https://doi.org/10.1126/science.abb3634· OSTI ID:1906831

Volland, Jean-Marie; Gonzalez-Rizzo, Silvina; Gros, Olivier; Tyml, Tomáš; Ivanova, Natalia; Schulz, Frederik; Goudeau, Danielle; Elisabeth, Nathalie H; Nath, Nandita; Udwary, Daniel; Malmstrom, Rex R; Guidi-Rontani, Chantal; Bolte-Kluge, Susanne; Davies, Karen M; Jean, Maïtena R; Mansot, Jean-Louis; Mouncey, Nigel J; Angert, Esther R; Woyke, Tanja; Date, Shailesh V

Cells of most bacterial species are around 2 micrometers in length, with some of the largest specimens reaching 750 micrometers. Using fluorescence, x-ray, and electron microscopy in conjunction with genome sequencing, we characterized Candidatus (Ca.) Thiomargarita magnifica, a bacterium that has an average cell length greater than 9000 micrometers and is visible to the naked eye. These cells grow orders of magnitude over theoretical limits for bacterial cell size, display unprecedented polyploidy of more than half a million copies of a very large genome, and undergo a dimorphic life cycle with asymmetric segregation of chromosomes into daughter cells. These features, along with compartmentalization of genomic material and ribosomes in translationally active organelles bound by bioenergetic membranes, indicate gain of complexity in the Thiomargarita lineage and challenge traditional concepts of bacterial cells.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER)
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 1906831
• Journal Information: Science (New York, N.Y.), Vol. 376, Issue 6600
• Country of Publication: United States
• Language: English