Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

High-Biomass Sorghums as a Feedstock for Renewable Fuels and Chemicals

Other · · Sorghum in the 21st Century: Food – Fodder – Feed – Fuel for a Rapidly Changing World
 [1];  [2]
  1. Univ. of Florida, Gainesville, FL (United States); Joint BioEnergy Institute, Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Univ. of Florida, Gainesville, FL (United States)

High-biomass sorghums are intended for use in biorefineries that convert vegetative biomass into renewable fuels and chemicals. The majority of plant biomass consists of cell walls, a complex matrix of cellulose, hemicellulosic polysaccharides and lignin. In the biorefinery, the biomass is ground and then subjected to a thermo-chemical pretreatment (elevated temperature, high pressure, chemical catalysts) that disrupts the cell wall matrix and solubilizes some of the cell wall polymers, followed by enzymatic saccharification of the cellulose, which produces D-glucose. The glucose and sometimes also the xylose derived from the hydrolysis of the hemicellulosic polysaccharides, are subsequently converted to fuels or other useful chemicals by microbial biocatalysts. The genetic improvement of high-biomass sorghums has as its ultimate goals to maximize the yield of fermentable sugars on a per-hectare basis, to minimize the inputs of fertilizer, irrigation, fungicides and pesticides and to reduce the environmental footprint. Breeding strategies thus need to target biomass yield, biomass composition, maturity, pest and disease resistance and nutrient use efficiency. Furthermore, this chapter reviews the genetic basis of these traits and their potential application in breeding programs.

Research Organization:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
DOE Contract Number:
SC0019097
OSTI ID:
1901823
Journal Information:
Sorghum in the 21st Century: Food – Fodder – Feed – Fuel for a Rapidly Changing World, Journal Name: Sorghum in the 21st Century: Food – Fodder – Feed – Fuel for a Rapidly Changing World
Country of Publication:
United States
Language:
English

References (134)

The Sorghum bicolor genome and the diversification of grasses journal January 2009
Performance evaluation of biomass sorghum in Hawaii and Texas journal September 2017
Fuel Versus Food journal October 2009
Inheritance and molecular mapping of anthracnose resistance genes present in sorghum line SC112-14 journal July 2014
Genetic Regulation of Development in Sorghum bicolar: I. Role of the Maturity Genes journal October 1986
Pretreatment and Lignocellulosic Chemistry journal May 2012
Loss of an MDR Transporter in Compact Stalks of Maize br2 and Sorghum dw3 Mutants journal October 2003
Genetic diversity and population structure analysis of accessions in the US historic sweet sorghum collection journal September 2009
Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties journal January 2013
Phytochrome B Represses Teosinte Branched1 Expression and Induces Sorghum Axillary Bud Outgrowth in Response to Light Signals journal January 2006
Brown midrib2 (Bmr2) encodes the major 4-coumarate:coenzyme A ligase involved in lignin biosynthesis in sorghum (Sorghum bicolor (L.) Moench): Brown midrib2 (Bmr2) encodes the major 4-coumarate:coenzyme A ligase journal April 2012
Microbial synthesis of n-butanol, isobutanol, and other higher alcohols from diverse resources journal May 2013
Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis journal September 2009
Genetic Control of a Photoperiod-Sensitive Response in Sorghum bicolor (L.) Moench journal January 1999
ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification journal April 2010
Molecular Breeding to Enhance Ethanol Production from Corn and Sorghum Stover journal January 2007
The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors journal August 2011
Ratoon cold tolerance of Pennisetum, Erianthus, and Saccharum bioenergy feedstocks journal December 2017
Forage and biomass feedstock production from hybrid forage sorghum and sorghum-sudangrass hybrids journal December 2008
Biomass production, water and nitrogen use efficiency in photoperiod-sensitive sorghum in the Texas High Plains journal March 2014
Molecular characterization of the pyrolysis of biomass. 2. Applications journal July 1987
Phenotype, Fiber Composition, and in vitro Dry Matter Disappearance of Chemically Induced Brown Midrib (bmr) Mutants of Sorghum1 journal January 1978
Tissue-Specific Patterns of Lignification Are Disturbed in the brown midrib2 Mutant of Maize ( Zea mays L.) journal February 2001
Genetic Enhancement of Sorghum for Biomass Utilization book January 2012
The Path Forward for Biofuels and Biomaterials journal January 2006
Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels journal June 2014
Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae journal December 2006
Identification of genomic regions affecting plant height in sorghum and maize journal March 1995
Applying genotyping (TILLING) and phenotyping analyses to elucidate gene function in a chemically induced sorghum mutant population journal January 2008
Comparative analysis of QTLs affecting plant height and maturity across the Poaceae, in reference to an interspecific sorghum population. journal September 1995
Pulsed Neutron Gamma Ray Logging for Minerals Associated with Uranium journal January 1981
Comparative analysis of pretreatment methods on sorghum (Sorghum durra) stalk agrowaste for holocellulose content journal July 2018
Gibberellin deficiency pleiotropically induces culm bending in sorghum: an insight into sorghum semi-dwarf breeding journal June 2014
p -Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon journal February 2014
The Effect of Tropical Sorghum Conversion and Inbred Development on Genome Diversity as Revealed by High-Resolution Genotyping journal January 2008
Molecular evidence for a single genetic clone of invasive Arundo donax in the United States journal February 2008
A High-Throughput Platform for Screening Milligram Quantities of Plant Biomass for Lignocellulose Digestibility journal January 2010
GIBBERELLIN BIOSYNTHESIS IN ZEA MAYS: THE 3-HYDROXYLATION STEP GA20 TO GA1 book January 1983
The Evolution of Photoperiod-Insensitive Flowering in Sorghum, A Genomic Model for Panicoid Grasses journal June 2016
High-parent heterosis for biomass yield in photoperiod-sensitive sorghum hybrids journal October 2014
Identification of genomic regions that affect grain-mould incidence and other traits of agronomic importance in sorghum journal February 2001
Population genomic and genome-wide association studies of agroclimatic traits in sorghum journal December 2012
Molecular mapping of Cg1, a gene for resistance to anthracnose (Colletotrichum sublineolum) in sorghum journal August 2008
Identification of genetic markers linked to anthracnose resistance in sorghum using association analysis journal March 2013
Pyrolysis-GC-MS characterization of forage materials journal August 1991
Genetic diversity in sorghum [Sorghum bicolor (L.) Moench] germplasm from Southern Africa as revealed by microsatellite markers and agro-morphological traits journal April 2016
Use of U.S. Croplands for Biofuels Increases Greenhouse Gases Through Emissions from Land-Use Change journal February 2008
Cloning and characterization of the maize An1 gene. journal January 1995
New Approach to Increasing Rice Lodging Resistance and Biomass Yield Through the Use of High Gibberellin Producing Varieties journal February 2014
Cellulose Biosynthesis: Current Views and Evolving Concepts journal May 2005
Lignin Structural Alterations in Thermochemical Pretreatments with Limited Delignification journal August 2015
Enhancing cellulose utilization for fuels and chemicals by genetic modification of plant cell wall architecture journal April 2015
Co-localization of quantitative trait loci for foliar disease resistance in sorghum journal October 2009
Inheritance of Height in Sorghum1 journal January 1954
Fourier Transform Infrared (FTIR) Study and Thermal Decomposition Kinetics of Sorghum bicolour Glume and Albizia pedicellaris Residues journal July 2014
The Genetics of Sorghum Improvement journal March 1975
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice journal August 2013
Qualitative and Quantitative Analyses of Gibberellins in Vegetative Shoots of Normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 Seedlings of Zea mays L. journal December 1988
Butanol production from renewable biomass by clostridia journal November 2012
A Genomewide Analysis of the Cinnamyl Alcohol Dehydrogenase Family in Sorghum [ Sorghum bicolor (L.) Moench] Identifies SbCAD2 as the Brown midrib6 Gene journal December 2008
Association mapping of brassinosteroid candidate genes and plant architecture in a diverse panel of Sorghum bicolor journal October 2014
High throughput determination of glucan and xylan fractions in lignocelluloses journal February 2011
An novel immobilization method of Saccharomyces cerevisiae to sorghum bagasse for ethanol production journal May 2007
Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2 journal March 2017
Pyrolysis-Gas Chromatography-Mass Spectrometry book January 1992
A potential role for sinapyl p-coumarate as a radical transfer mechanism in grass lignin formation journal July 2008
FAO's Director-General on How to Feed the World in 2050 journal December 2009
Quantification of yield losses caused by leaf anthracnose on sorghum in Brazil journal May 2017
Crystallographic snapshot of cellulose synthesis and membrane translocation journal December 2012
Three FLOWERING LOCUS T -like genes function as potential florigens and mediate photoperiod response in sorghum journal January 2016
Genetic diversity and pathotype determination of Colletotrichum sublineolum isolates causing anthracnose in sorghum journal January 2012
Identification and Characterization of Four Missense Mutations in Brown midrib 12 (Bmr12), the Caffeic O-Methyltranferase (COMT) of Sorghum journal April 2012
Fourier-Transform Raman and Fourier-Transform Infrared Spectroscopy (An Investigation of Five Higher Plant Cell Walls and Their Components) journal December 1994
Identification and characterization of RAPD and SCAR markers linked to anthracnose resistance gene in sorghum [Sorghum bicolor (L.) Moench] journal November 2006
Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover report June 2002
Evidence for an intramembrane component associated with a cellulose microfibril-synthesizing complex in higher plants journal February 1980
High-biomass C 4 grasses—Filling the yield gap journal August 2017
Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents journal August 2009
Evaluation and Characterization of Forage Sorghum as Feedstock for Fermentable Sugar Production journal August 2008
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth journal January 1993
Rapid analysis of composition and reactivity in cellulosic biomass feedstocks with near-infrared spectroscopy journal March 2015
The Structure of the Catalytic Domain of a Plant Cellulose Synthase and Its Assembly into Dimers journal July 2014
Functional conservation of the glycosyltransferase gene GT47A in the monocot rice journal April 2014
Techno-Economic Evaluation of Cellulosic Ethanol Production Based on Pilot Biorefinery Data: a Case Study of Sweet Sorghum Bagasse Processed via L+SScF journal April 2018
Characterization of Novel Sorghum brown midrib Mutants from an EMS-Mutagenized Population journal September 2014
The Maturity Genes of Sorghum book January 1967
Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose journal December 2007
Consensus and Contention in the Food-Versus-Fuel Debate journal October 2014
The effect of row spacing and seeding rate on biomass production and plant stand characteristics of non-irrigated photoperiod-sensitive sorghum (Sorghum bicolor (L.) Moench) journal May 2012
Using Genotyping by Sequencing to Map Two Novel Anthracnose Resistance Loci in Sorghum bicolor journal May 2016
Tertiary model of a plant cellulose synthase journal April 2013
Genetics and Reproduction of Common (Phragmites australis) and Giant Reed (Arundo donax) journal December 2010
Cellulose Synthesis in Higher Plants journal November 2006
Sorghum Dw2 Encodes a Protein Kinase Regulator of Stem Internode Length journal July 2017
Genetic Improvement of Sorghum as a Biofuel Feedstock: II. QTL for Stem and Leaf Structural Carbohydrates journal January 2008
Net energy of cellulosic ethanol from switchgrass journal January 2008
A sorghum diversity panel biofuel feedstock screen for genotypes with high hydrolysis yield potential journal May 2010
Fourth Maturity Gene Locus in Sorghum1 journal January 1966
Efficient Mapping of Plant Height Quantitative Trait Loci in a Sorghum Association Population With Introgressed Dwarfing Genes journal August 2008
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning journal November 2011
Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum : Characterization of novel sorghum journal October 2015
() Represses Sorghum Flowering in Long Days: Alleles Enhance Biomass Accumulation and Grain Production journal January 2014
Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses journal November 2019
The Enzyme Activity and Substrate Specificity of Two Major Cinnamyl Alcohol Dehydrogenases in Sorghum ( Sorghum bicolor ), SbCAD2 and SbCAD4 journal June 2017
Development of Leaf Anthracnose and its Effecton Yield and Grain Weight of Sorghum in West Africa journal January 1996
Genomic Dissection of Anthracnose (Colletotrichum sublineolum) Resistance Response in Sorghum Differential Line SC112-14 journal April 2020
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity journal June 2012
A Role for Brassinosteroids in Light-Dependent Development of Arabidopsis journal April 1996
The Physiological, Biochemical and Molecular Roles of Brassinosteroids and Salicylic Acid in Plant Processes and Salt Tolerance journal May 2010
Global land use change, economic globalization, and the looming land scarcity journal February 2011
Methodology for enabling high-throughput simultaneous saccharification and fermentation screening of yeast using solid biomass as a substrate journal January 2015
A candidate-gene approach to clone the sorghum Brown midrib gene encoding caffeic acid O-methyltransferase journal March 2003
Retrospective genomic analysis of sorghum adaptation to temperate-zone grain production journal June 2013
An Introduction to Pyrolysis Mass Spectrometry of Lingnocellulosic Material: Case Studies on Barley Straw, Corn Stem and Agropyron book January 1989
Performance of 15 Genotypes at Five Sites in Europe journal January 2001
Genomics of plant cell wall biogenesis journal June 2005
A Nonsense Mutation in a Cinnamyl Alcohol Dehydrogenase Gene Is Responsible for the Sorghum brown midrib6 Phenotype journal April 2009
Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment journal September 2010
Biofuels 2020: Biorefineries based on lignocellulosic materials journal July 2016
A Novel Cytochrome P450 Is Implicated in Brassinosteroid Biosynthesis via the Characterization of a Rice Dwarf Mutant, dwarf11, with Reduced Seed Length journal February 2005
High biomass yield energy sorghum: developing a genetic model for C4 grass bioenergy crops: Modeling and Analysis: Developing a genetic model for C4 grass bioenergy crops journal September 2012
The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis journal February 1999
Coincident light and clock regulation of pseudoresponse regulator protein 37 (PRR37) controls photoperiodic flowering in sorghum journal September 2011
Allelic Association, Chemical Characterization and Saccharification Properties of brown midrib Mutants of Sorghum (Sorghum bicolor (L.) Moench) journal October 2008
Cellulose Synthase-Like CslF Genes Mediate the Synthesis of Cell Wall (1,3;1,4)- -D-Glucans journal March 2006
The Sorghum Photoperiod Sensitivity Gene, Ma3, Encodes a Phytochrome B journal February 1997
Sorghum Phytochrome B Inhibits Flowering in Long Days by Activating Expression of SbPRR37 and SbGHD7, Repressors of SbEHD1, SbCN8 and SbCN12 journal August 2014
Effect of Height on Yield and Yield Components of Two Isogenic Lines of Sorghum vulgare Pers.1 journal January 1966
Analysis of Crystallinity Index and Hydrolysis Rates in the Bioenergy Crop Sorghum bicolor journal August 2011
Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1) journal October 2011
Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release journal February 2010
Potential for ethanol production from different sorghum cultivars journal December 2017
Maize Tricin-Oligolignol Metabolites and their Implications for Monocot Lignification journal April 2016
The maize Brown midrib1 locus affects cell wall composition and plant development in a dose-dependent manner journal May 2002