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Picoliter droplet sample processing and deposition for mass spectrometry

Patent ·
OSTI ID:1893061
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Disclosed herein include systems, devices, and methods for droplet deposition for mass spectrometry. In some embodiments, a microfluidic device comprising wells is reversibly sealed to a mass spectrometry surface and used to deposit contents of droplets (e.g., enzymes and substrates), or products thereof, onto the mass spectrometry surface. The contents of droplets can be analyzed by laser desorption/ionization to, for example, identify a substrate of an enzyme or an enzyme capable of catalyzing a substrate to a product.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
11,366,122
Application Number:
17/062,609
OSTI ID:
1893061
Country of Publication:
United States
Language:
English

References (6)

Clathrate nanostructures for mass spectrometry journal October 2007
Encoding substrates with mass tags to resolve stereospecific reactions using Nimzyme: Encoding substrates with mass tags using Nimzyme journal February 2012
A nanostructure-initiator mass spectrometry-based enzyme activity assay journal March 2008
High-throughput platforms for metabolomics journal February 2016
Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis journal July 2008
Combinatorial drug discovery in nanoliter droplets journal June 2018

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