Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Methods and systems to produce lignin-modifying enzymes and uses thereof

Patent ·
OSTI ID:1892867
Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, the lignin-modifying enzymes are lignin-degrading enzymes capable of breaking down lignin into component parts that are usable for other purposes. Several embodiments are directed to systems for producing lignin-modifying enzymes in vivo, including in yeast and/or plant species, and certain embodiments are directed to methods of creating these systems, including transfecting the species to produce lignin-modifying enzymes.
Research Organization:
Stanford Univ., CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
SC0014112
Assignee:
The Board of Trustees of the Leland Stanford Junior University (Stanford, CA)
Patent Number(s):
11,312,753
Application Number:
16/582,814
OSTI ID:
1892867
Country of Publication:
United States
Language:
English

References (49)

Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae journal August 2009
Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution journal March 2018
Homologous and Heterologous Expression of Basidiomycete Genes Related to Plant Biomass Degradation book April 2016
Production and field performance of transgenic alfalfa (Medicago sativa L.) expressing alpha-amylase and manganese-dependent lignin peroxidase book January 1995
Peroxidases in the genus Nicotiana journal January 1970
Hydrogen-peroxide-producing system ofPleurotus eryngii involving the extracellular enzyme aryl-alcohol oxidase journal June 1994
Fungal aryl-alcohol oxidase: a peroxide-producing flavoenzyme involved in lignin degradation journal January 2012
Senescence-Related Changes in the Leaf Apoplast journal November 2013
Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli journal May 2012
In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium journal April 1991
Lignin peroxidase of Phanerochaete chrysosporium book January 1988
Ligninolysis by a purified lignin peroxidase journal June 1993
Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators. journal November 1992
Lignin peroxidase compound III. Mechanism of formation and decomposition. journal February 1990
Functional Expression of Horseradish Peroxidase in E. coli by Directed Evolution journal June 1999
Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78 journal May 2004
Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana) journal December 2017
A dye-decolorizing peroxidase from Bacillus subtilis exhibiting substrate-dependent optimum temperature for dyes and β-ether lignin dimer journal February 2015
Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers journal January 2015
Development of novel assays for lignin degradation: comparative analysis of bacterial and fungal lignin degraders journal January 2010
Ligninase of Phanerochaete chrysosporium. Mechanism of its degradation of the non-phenolic arylglycerol β-aryl ether substructure of lignin journal May 1986
pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves: DsRed and GFP vectors for co-expression in plants journal August 2002
Identification and treatment of heme depletion attributed to overexpression of a lineage of evolved P450 monooxygenases journal November 2012
Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi journal June 2014
Comparative genomics of biotechnologically important yeasts journal August 2016
Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase. journal April 1990
Lignin-degrading Peroxidases from Genome of Selective Ligninolytic Fungus Ceriporiopsis subvermispora journal March 2012
Progress and obstacles in the production and application of recombinant lignin-degrading peroxidases journal April 2016
Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution journal October 2017
Multiple Molecular Forms of Peroxidases and Esterases Among Nicotiana Species and Amphiploids journal September 1970
A R2R3-MYB GeneLfMYB113is Responsible for Autumn Leaf Coloration in Formosan sweet gum (Liquidambar formosanaHance) journal January 2017
Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris journal May 2000
Nicotiana benthamiana : Its History and Future as a Model for Plant–Pathogen Interactions journal August 2008
Disruption of LACCASE4 and 17 Results in Tissue-Specific Alterations to Lignification of Arabidopsis thaliana Stems journal March 2011
Purification and Catalytic Properties of Two Manganese Peroxidase Isoenzymes from Pleurotus eryngii journal April 1996
Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed journal January 2006
pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants journal September 2009
Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD , LigF and LigG from Sphingomonas paucimobilis SYK-6 journal November 2016
Lignin Valorization: Improving Lignin Processing in the Biorefinery journal May 2014
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone journal September 2015
Homologous Expression of Recombinant Lignin Peroxidase in Phanerochaete chrysosporium journal April 1999
Studies on the Production of Fungal Peroxidases in Aspergillus niger journal July 2000
Rapid Degradation of Isolated Lignins by Phanerochaete chrysosporium journal June 1983
Pyranose Oxidase, a Major Source of H 2 O 2 during Wood Degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida journal July 1994
Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability journal January 2014
Improved Efficiency in Screening for Lignin-Modifying Peroxidases and Laccases of Basidiomycetes journal April 2017
Pyridine Hemochromagen Assay for Determining the Concentration of Heme in Purified Protein Solutions journal January 2015
Lignin Engineering in Forest Trees journal July 2019
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example journal January 2014