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Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression

Patent ·
OSTI ID:1892815
The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
11,299,519
Application Number:
16/831,649
OSTI ID:
1892815
Country of Publication:
United States
Language:
English

References (6)

Protein tolerance to random amino acid change journal June 2004
The Salmonella enterica sv. Typhimurium smvA, yddG and ompD (porin) genes are required for the efficient efflux of methyl viologen: Resistance of S. enterica sv. Typhimurium to methyl viologen journal October 2002
Global transcriptome response to ionic liquid by a tropical rain forest soil bacterium, Enterobacter lignolyticus journal May 2012
An auto-inducible mechanism for ionic liquid resistance in microbial biofuel production journal March 2014
Complete genome sequence of “Enterobacter lignolyticus” SCF1 journal September 2011
KmrA Multidrug Efflux Pump from Klebsiella pneumoniae journal January 2006

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