Media for culturing epithelial cells

Patent · 05 April 2022

OSTI ID:1892790

LaBarge, Mark A.; Stampfer, Martha R.; Garbe, James C.

A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE; National Institutes of Health (NIH)
• DOE Contract Number: AC02-05CH11231
• Assignee: The Regents of the University of California (Oakland, CA)
• Patent Number(s): 11,293,008
• Application Number: 15/762,517
• OSTI ID: 1892790
• Country of Publication: United States
• Language: English