Architecture of the cytoplasmic face of the nuclear pore

Bley, Christopher J.; Nie, Si; Mobbs, George W.; Petrovic, Stefan; Gres, Anna T.; Liu, Xiaoyu; Mukherjee, Somnath; Harvey, Sho; Huber, Ferdinand M.; Lin, Daniel H.; Brown, Bonnie; Tang, Aaron W.; Rundlet, Emily J.; Correia, Ana R.; Chen, Shane; Regmi, Saroj G.; Stevens, Taylor A.; Jette, Claudia A.; Dasso, Mary; Patke, Alina; Palazzo, Alexander F.; Kossiakoff, Anthony A.; Hoelz, Andre

doi:10.1126/science.abm9129

Title: Architecture of the cytoplasmic face of the nuclear pore

Journal Article · 01 June 2022 · Science

DOI:https://doi.org/10.1126/science.abm9129· OSTI ID:1876058

Bley, Christopher J.; Nie, Si; Mobbs, George W.; Petrovic, Stefan; Gres, Anna T.; Liu, Xiaoyu; Mukherjee, Somnath; Harvey, Sho; Huber, Ferdinand M.; Lin, Daniel H.; Brown, Bonnie; Tang, Aaron W.; Rundlet, Emily J.; Correia, Ana R.; Chen, Shane; Regmi, Saroj G.; Stevens, Taylor A.; Jette, Claudia A.; Dasso, Mary; Patke, Alina more »

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: DOE - Office Of Science; HHMI; National Institutes of Health (NIH); OTHER

OSTI ID:: 1876058

Journal Information:: Science, Vol. 376

Country of Publication:: United States

Language:: ENGLISH

References (143)

The Structure of the Nuclear Pore Complex Hoelz, André; Debler, Erik W.; Blobel, Günter Annual Review of Biochemistry, Vol. 80, Issue 1 https://doi.org/10.1146/annurev-biochem-060109-151030	journal	July 2011
The Structure of the Nuclear Pore Complex (An Update) Lin, Daniel H.; Hoelz, André Annual Review of Biochemistry, Vol. 88, Issue 1 https://doi.org/10.1146/annurev-biochem-062917-011901	journal	June 2019
Structure and Assembly of the Nuclear Pore Complex Hampoelz, Bernhard; Andres-Pons, Amparo; Kastritis, Panagiotis Annual Review of Biophysics, Vol. 48, Issue 1 https://doi.org/10.1146/annurev-biophys-052118-115308	journal	May 2019
The Nuclear Pore Complex as a Flexible and Dynamic Gate Knockenhauer, Kevin E.; Schwartz, Thomas U. Cell, Vol. 164, Issue 6 https://doi.org/10.1016/j.cell.2016.01.034	journal	March 2016
Viral Subversion of Nucleocytoplasmic Trafficking: Viral Subversion of Nucleocytoplasmic Trafficking Yarbrough, Melanie L.; Mata, Miguel A.; Sakthivel, Ramanavelan Traffic, Vol. 15, Issue 2 https://doi.org/10.1111/tra.12137	journal	December 2013
Gene Regulation by Nucleoporins and Links to Cancer Köhler, Alwin; Hurt, Ed Molecular Cell, Vol. 38, Issue 1 https://doi.org/10.1016/j.molcel.2010.01.040	journal	April 2010
Structural Biology of Nucleocytoplasmic Transport Cook, Atlanta; Bono, Fulvia; Jinek, Martin Annual Review of Biochemistry, Vol. 76, Issue 1 https://doi.org/10.1146/annurev.biochem.76.052705.161529	journal	June 2007
Nuclear import by karyopherin-βs: Recognition and inhibition Chook, Yuh Min; Süel, Katherine E. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1813, Issue 9 https://doi.org/10.1016/j.bbamcr.2010.10.014	journal	September 2011
Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex Chug, Hema; Trakhanov, Sergei; Hülsmann, Bastian B. Science, Vol. 350, Issue 6256 https://doi.org/10.1126/science.aac7420	journal	August 2015
Architecture of the fungal nuclear pore inner ring complex Stuwe, Tobias; Bley, Christopher J.; Thierbach, Karsten Science, Vol. 350, Issue 6256 https://doi.org/10.1126/science.aac9176	journal	August 2015
Linker Nups connect the nuclear pore complex inner ring with the outer ring and transport channel Fischer, Jessica; Teimer, Roman; Amlacher, Stefan Nature Structural & Molecular Biology, Vol. 22, Issue 10 https://doi.org/10.1038/nsmb.3084	journal	September 2015
The Three-Dimensional Structure of the Autoproteolytic, Nuclear Pore-Targeting Domain of the Human Nucleoporin Nup98 Hodel, Alec E.; Hodel, Mary R.; Griffis, Eric R. Molecular Cell, Vol. 10, Issue 2 https://doi.org/10.1016/S1097-2765(02)00589-0	journal	August 2002
Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex Berke, Ian C.; Boehmer, Thomas; Blobel, Günter The Journal of Cell Biology, Vol. 167, Issue 4 https://doi.org/10.1083/jcb.200408109	journal	November 2004
Architecture of a Coat for the Nuclear Pore Membrane Hsia, Kuo-Chiang; Stavropoulos, Pete; Blobel, Günter Cell, Vol. 131, Issue 7 https://doi.org/10.1016/j.cell.2007.11.038	journal	December 2007
Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats Brohawn, S. G.; Leksa, N. C.; Spear, E. D. Science, Vol. 322, Issue 5906 https://doi.org/10.1126/science.1165886	journal	November 2008
Structural and Functional Studies of Nup107/Nup133 Interaction and Its Implications for the Architecture of the Nuclear Pore Complex Boehmer, Thomas; Jeudy, Sandra; Berke, Ian C. Molecular Cell, Vol. 30, Issue 6 https://doi.org/10.1016/j.molcel.2008.04.022	journal	June 2008
A Fence-like Coat for the Nuclear Pore Membrane Debler, Erik W.; Ma, Yingli; Seo, Hyuk-Soo Molecular Cell, Vol. 32, Issue 6 https://doi.org/10.1016/j.molcel.2008.12.001	journal	December 2008
The Structure of the Scaffold Nucleoporin Nup120 Reveals a New and Unexpected Domain Architecture Leksa, Nina C.; Brohawn, Stephen G.; Schwartz, Thomas U. Structure, Vol. 17, Issue 8 https://doi.org/10.1016/j.str.2009.06.003	journal	August 2009
Architectural Nucleoporins Nup157/170 and Nup133 Are Structurally Related and Descend from a Second Ancestral Element Whittle, James R. R.; Schwartz, Thomas U. Journal of Biological Chemistry, Vol. 284, Issue 41 https://doi.org/10.1074/jbc.M109.023580	journal	August 2009
Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex Seo, H. -S.; Ma, Y.; Debler, E. W. Proceedings of the National Academy of Sciences, Vol. 106, Issue 34 https://doi.org/10.1073/pnas.0907453106	journal	August 2009
Molecular architecture of the Nup84–Nup145C–Sec13 edge element in the nuclear pore complex lattice Brohawn, Stephen G.; Schwartz, Thomas U. Nature Structural & Molecular Biology, Vol. 16, Issue 11 https://doi.org/10.1038/nsmb.1713	journal	October 2009
Structure of a trimeric nucleoporin complex reveals alternate oligomerization states Nagy, V.; Hsia, K. -C.; Debler, E. W. Proceedings of the National Academy of Sciences, Vol. 106, Issue 42 https://doi.org/10.1073/pnas.0909373106	journal	October 2009
Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex Bilokapic, S.; Schwartz, T. U. Proceedings of the National Academy of Sciences, Vol. 109, Issue 38 https://doi.org/10.1073/pnas.1205151109	journal	September 2012
Structural and Functional Studies of the 252 kDa Nucleoporin ELYS Reveal Distinct Roles for Its Three Tethered Domains Bilokapic, Silvija; Schwartz, Thomas U. Structure, Vol. 21, Issue 4 https://doi.org/10.1016/j.str.2013.02.006	journal	April 2013
Crystal structure of human nuclear pore complex component NUP43 Xu, Chao; Li, Zhihong; He, Hao FEBS Letters, Vol. 589, Issue 21 https://doi.org/10.1016/j.febslet.2015.09.008	journal	September 2015
Architecture of the nuclear pore complex coat Stuwe, T.; Correia, A. R.; Lin, D. H. Science, Vol. 347, Issue 6226 https://doi.org/10.1126/science.aaa4136	journal	February 2015
Atomic structure of the Y complex of the nuclear pore Kelley, Kotaro; Knockenhauer, Kevin E.; Kabachinski, Greg Nature Structural & Molecular Biology, Vol. 22, Issue 5 https://doi.org/10.1038/nsmb.2998	journal	March 2015
Toward the atomic structure of the nuclear pore complex: when top down meets bottom up Hoelz, André; Glavy, Joseph S.; Beck, Martin Nature Structural & Molecular Biology, Vol. 23, Issue 7 https://doi.org/10.1038/nsmb.3244	journal	June 2016
Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold Bui, Khanh Huy; von Appen, Alexander; DiGuilio, Amanda L. Cell, Vol. 155, Issue 6 https://doi.org/10.1016/j.cell.2013.10.055	journal	December 2013
The Crystal Structure of Mouse Nup35 Reveals Atypical RNP Motifs and Novel Homodimerization of the RRM Domain Handa, Noriko; Kukimoto-Niino, Mutsuko; Akasaka, Ryogo Journal of Molecular Biology, Vol. 363, Issue 1 https://doi.org/10.1016/j.jmb.2006.07.089	journal	October 2006
Crystal Structure of Nucleoporin Nic96 Reveals a Novel, Intricate Helical Domain Architecture Jeudy, Sandra; Schwartz, Thomas U. Journal of Biological Chemistry, Vol. 282, Issue 48 https://doi.org/10.1074/jbc.M705479200	journal	September 2007
Structural Basis of the Nic96 Subcomplex Organization in the Nuclear Pore Channel Schrader, Nils; Stelter, Philipp; Flemming, Dirk Molecular Cell, Vol. 29, Issue 1 https://doi.org/10.1016/j.molcel.2007.10.022	journal	January 2008
Structure and nucleic acid binding activity of the nucleoporin Nup157 Seo, H. -S.; Blus, B. J.; Jankovic, N. Z. Proceedings of the National Academy of Sciences, Vol. 110, Issue 41 https://doi.org/10.1073/pnas.1316607110	journal	September 2013
Structure, Dynamics, Evolution, and Function of a Major Scaffold Component in the Nuclear Pore Complex Sampathkumar, Parthasarathy; Kim, Seung Joong; Upla, Paula Structure, Vol. 21, Issue 4 https://doi.org/10.1016/j.str.2013.02.005	journal	April 2013
Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192 and karyopherins Stuwe, T.; Lin, D. H.; Collins, L. N. Proceedings of the National Academy of Sciences, Vol. 111, Issue 7 https://doi.org/10.1073/pnas.1311081111	journal	February 2014
Architecture of the symmetric core of the nuclear pore Lin, D. H.; Stuwe, T.; Schilbach, S. Science, Vol. 352, Issue 6283 https://doi.org/10.1126/science.aaf1015	journal	April 2016
Molecular architecture of the inner ring scaffold of the human nuclear pore complex Kosinski, J.; Mosalaganti, S.; von Appen, A. Science, Vol. 352, Issue 6283 https://doi.org/10.1126/science.aaf0643	journal	April 2016
Integrative structure and functional anatomy of a nuclear pore complex Kim, Seung Joong; Fernandez-Martinez, Javier; Nudelman, Ilona Nature, Vol. 555, Issue 7697 https://doi.org/10.1038/nature26003	journal	March 2018
In-cell architecture of the nuclear pore and snapshots of its turnover Allegretti, Matteo; Zimmerli, Christian E.; Rantos, Vasileios Nature, Vol. 586, Issue 7831 https://doi.org/10.1038/s41586-020-2670-5	journal	September 2020
Architecture of the linker-scaffold in the nuclear pore Petrovic, Stefan; Samanta, Dipanjan; Perriches, Thibaud Science, Vol. 376, Issue 6598 https://doi.org/10.1126/science.abm9798	journal	June 2022
In situ structural analysis of the human nuclear pore complex von Appen, Alexander; Kosinski, Jan; Sparks, Lenore Nature, Vol. 526, Issue 7571 https://doi.org/10.1038/nature15381	journal	September 2015
Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis Huang, Gaoxingyu; Zhang, Yanqing; Zhu, Xuechen Cell Research, Vol. 30, Issue 6 https://doi.org/10.1038/s41422-020-0319-4	journal	May 2020
A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. Grandi, P.; Emig, S.; Weise, C. Journal of Cell Biology, Vol. 130, Issue 6 https://doi.org/10.1083/jcb.130.6.1263	journal	September 1995
Nup116p and Nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor Gle2p Bailer, S. M. The EMBO Journal, Vol. 17, Issue 4 https://doi.org/10.1093/emboj/17.4.1107	journal	February 1998
Functional Characterization of a Nup159p-containing Nuclear Pore Subcomplex Belgareh, Naı̈ma; Snay-Hodge, Christine; Pasteau, Fabien Molecular Biology of the Cell, Vol. 9, Issue 12 https://doi.org/10.1091/mbc.9.12.3475	journal	December 1998
Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p Schmitt, C. The EMBO Journal, Vol. 18, Issue 15 https://doi.org/10.1093/emboj/18.15.4332	journal	August 1999
Assembly and Preferential Localization of Nup116p on the Cytoplasmic Face of the Nuclear Pore Complex by Interaction with Nup82p Ho, Albert K.; Shen, Tian Xiang; Ryan, Kathryn J. Molecular and Cellular Biology, Vol. 20, Issue 15 https://doi.org/10.1128/MCB.20.15.5736-5748.2000	journal	August 2000
The N-Terminal Domain of Nup159 Forms a β-Propeller that Functions in mRNA Export by Tethering the Helicase Dbp5 to the Nuclear Pore Weirich, Christine S.; Erzberger, Jan P.; Berger, James M. Molecular Cell, Vol. 16, Issue 5 https://doi.org/10.1016/j.molcel.2004.10.032	journal	December 2004
Inositol hexakisphosphate and Gle1 activate the DEAD-box protein Dbp5 for nuclear mRNA export Alcázar-Román, Abel R.; Tran, Elizabeth J.; Guo, Shuangli Nature Cell Biology, Vol. 8, Issue 7 https://doi.org/10.1038/ncb1427	journal	June 2006
Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN Napetschnig, J.; Blobel, G.; Hoelz, A. Proceedings of the National Academy of Sciences, Vol. 104, Issue 6 https://doi.org/10.1073/pnas.0610828104	journal	January 2007
Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19 Napetschnig, J.; Kassube, S. A.; Debler, E. W. Proceedings of the National Academy of Sciences, Vol. 106, Issue 9 https://doi.org/10.1073/pnas.0813267106	journal	February 2009
The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner von Moeller, Holger; Basquin, Claire; Conti, Elena Nature Structural & Molecular Biology, Vol. 16, Issue 3 https://doi.org/10.1038/nsmb.1561	journal	February 2009
Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1 Ren, Yi; Seo, Hyuk-Soo; Blobel, Günter Proceedings of the National Academy of Sciences, Vol. 107, Issue 23 https://doi.org/10.1073/pnas.1005389107	journal	May 2010
A conserved mechanism of DEAD-box ATPase activation by nucleoporins and InsP6 in mRNA export Montpetit, Ben; Thomsen, Nathan D.; Helmke, Kara J. Nature, Vol. 472, Issue 7342 https://doi.org/10.1038/nature09862	journal	March 2011
Structural and functional analysis of an essential nucleoporin heterotrimer on the cytoplasmic face of the nuclear pore complex Yoshida, Kimihisa; Seo, Hyuk-Soo; Debler, Erik W. Proceedings of the National Academy of Sciences, Vol. 108, Issue 40 https://doi.org/10.1073/pnas.1112846108	journal	September 2011
Molecular Basis for the Anchoring of Proto-Oncoprotein Nup98 to the Cytoplasmic Face of the Nuclear Pore Complex Stuwe, Tobias; Schada von Borzyskowski, Lennart; Davenport, Andrew M. Journal of Molecular Biology, Vol. 419, Issue 5 https://doi.org/10.1016/j.jmb.2012.03.024	journal	June 2012
Structural basis for assembly and function of the Nup82 complex in the nuclear pore scaffold Gaik, Monika; Flemming, Dirk; von Appen, Alexander The Journal of Cell Biology, Vol. 208, Issue 3 https://doi.org/10.1083/jcb.201411003	journal	February 2015
A short linear motif in scaffold Nup145C connects Y-complex with pre-assembled outer ring Nup82 complex Teimer, Roman; Kosinski, Jan; von Appen, Alexander Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01160-9	journal	October 2017
Structural and functional analysis of mRNA export regulation by the nuclear pore complex Lin, Daniel H.; Correia, Ana R.; Cai, Sarah W. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-04459-3	journal	June 2018
Structure and Function of the Nuclear Pore Complex Cytoplasmic mRNA Export Platform Fernandez-Martinez, Javier; Kim, Seung Joong; Shi, Yi Cell, Vol. 167, Issue 5 https://doi.org/10.1016/j.cell.2016.10.028	journal	November 2016
Activation of the DExD/H-box protein Dbp5 by the nuclear-pore protein Gle1 and its coactivator InsP6 is required for mRNA export Weirich, Christine S.; Erzberger, Jan P.; Flick, Jeffrey S. Nature Cell Biology, Vol. 8, Issue 7 https://doi.org/10.1038/ncb1424	journal	June 2006
Role of Nucleoporin Induction in Releasing an mRNA Nuclear Export Block Enninga, Jost; Levy, David E.; Blobel, Günter Science, Vol. 295, Issue 5559 https://doi.org/10.1126/science.1067861	journal	February 2002
RanBP2/Nup358 Provides a Major Binding Site for NXF1-p15 Dimers at the Nuclear Pore Complex and Functions in Nuclear mRNA Export Forler, Daniel; Rabut, Gwénaël; Ciccarelli, Francesca D. Molecular and Cellular Biology, Vol. 24, Issue 3 https://doi.org/10.1128/MCB.24.3.1155-1167.2004	journal	February 2004
Nup214 Is Required for CRM1-Dependent Nuclear Protein Export In Vivo Hutten, Saskia; Kehlenbach, Ralph H. Molecular and Cellular Biology, Vol. 26, Issue 18 https://doi.org/10.1128/MCB.00342-06	journal	September 2006
Ran-dependent docking of importin-β to RanBP2/Nup358 filaments is essential for protein import and cell viability Hamada, Masakazu; Haeger, Anna; Jeganathan, Karthik B. Journal of Cell Biology, Vol. 194, Issue 4 https://doi.org/10.1083/jcb.201102018	journal	August 2011
Vesiculoviral matrix (M) protein occupies nucleic acid binding site at nucleoporin pair (Rae1bulletNup98) Quan, B.; Seo, H. -S.; Blobel, G. Proceedings of the National Academy of Sciences, Vol. 111, Issue 25 https://doi.org/10.1073/pnas.1409076111	journal	June 2014
Nup358, a Cytoplasmically Exposed Nucleoporin with Peptide Repeats, Ran-GTP Binding Sites, Zinc Fingers, a Cyclophilin A Homologous Domain, and a Leucine-rich Region Wu, Jian; Matunis, Michael J.; Kraemer, Doris Journal of Biological Chemistry, Vol. 270, Issue 23 https://doi.org/10.1074/jbc.270.23.14209	journal	June 1995
A giant nucleopore protein that binds Ran/TC4 Yokoyama, N.; Hayashi, N.; Seki, T. Nature, Vol. 376, Issue 6536 https://doi.org/10.1038/376184a0	journal	July 1995
The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism Rout, Michael P.; Aitchison, John D.; Suprapto, Adisetyantari The Journal of Cell Biology, Vol. 148, Issue 4 https://doi.org/10.1083/jcb.148.4.635	journal	February 2000
Proteomic analysis of the mammalian nuclear pore complex Cronshaw, Janet M.; Krutchinsky, Andrew N.; Zhang, Wenzhu The Journal of Cell Biology, Vol. 158, Issue 5 https://doi.org/10.1083/jcb.200206106	journal	August 2002
The nucleoporin Nup358 associates with and regulates interphase microtubules Joseph, Jomon; Dasso, Mary FEBS Letters, Vol. 582, Issue 2 https://doi.org/10.1016/j.febslet.2007.11.087	journal	December 2007
Crystal Structure of the N-Terminal Domain of Nup358/RanBP2 Kassube, Susanne A.; Stuwe, Tobias; Lin, Daniel H. Journal of Molecular Biology, Vol. 423, Issue 5 https://doi.org/10.1016/j.jmb.2012.08.026	journal	November 2012
Generating conformation-specific synthetic antibodies to trap proteins in selected functional states Paduch, Marcin; Koide, Akiko; Uysal, Serdar Methods, Vol. 60, Issue 1 https://doi.org/10.1016/j.ymeth.2012.12.010	journal	March 2013
Using Dali for Protein Structure Comparison Holm, Liisa Methods in Molecular Biology https://doi.org/10.1007/978-1-0716-0270-6_3	book	January 2020
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis Hampoelz, Bernhard; Schwarz, Andre; Ronchi, Paolo Cell, Vol. 179, Issue 3 https://doi.org/10.1016/j.cell.2019.09.022	journal	October 2019
Two distinct classes of Ran-binding sites on the nucleoporin Nup-358 Yaseen, Nabeel R.; Blobel, Günter Proceedings of the National Academy of Sciences, Vol. 96, Issue 10 https://doi.org/10.1073/pnas.96.10.5516	journal	May 1999
Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain Nakielny, S. The EMBO Journal, Vol. 18, Issue 7 https://doi.org/10.1093/emboj/18.7.1982	journal	April 1999
Nup50, a Nucleoplasmically Oriented Nucleoporin with a Role in Nuclear Protein Export Guan, Tinglu; Kehlenbach, Ralph H.; Schirmer, Eric C. Molecular and Cellular Biology, Vol. 20, Issue 15 https://doi.org/10.1128/MCB.20.15.5619-5630.2000	journal	August 2000
Molecular Characterization of the Ran-binding Zinc Finger Domain of Nup153 Higa, Meda M.; Alam, Steven L.; Sundquist, Wesley I. Journal of Biological Chemistry, Vol. 282, Issue 23 https://doi.org/10.1074/jbc.M702715200	journal	June 2007
The Crystal Structure of the Ran-Nup153ZnF2 Complex: a General Ran Docking Site at the Nuclear Pore Complex Schrader, Nils; Koerner, Carolin; Koessmeier, Katja Structure, Vol. 16, Issue 7 https://doi.org/10.1016/j.str.2008.03.014	journal	July 2008
Crystallographic and Biochemical Analysis of the Ran-binding Zinc Finger Domain Partridge, James R.; Schwartz, Thomas U. Journal of Molecular Biology, Vol. 391, Issue 2 https://doi.org/10.1016/j.jmb.2009.06.011	journal	August 2009
Cell type‐specific nuclear pores: a case in point for context‐dependent stoichiometry of molecular machines Ori, Alessandro; Banterle, Niccolò; Iskar, Murat Molecular Systems Biology, Vol. 9, Issue 1 https://doi.org/10.1038/msb.2013.4	journal	January 2013
Nup358 integrates nuclear envelope breakdown with kinetochore assembly Salina, Davide; Enarson, Paul; Rattner, J. B. Journal of Cell Biology, Vol. 162, Issue 6 https://doi.org/10.1083/jcb.200304080	journal	September 2003
Quantification of cell cycle kinetics by EdU (5-ethynyl-2′-deoxyuridine)-coupled-fluorescence-intensity analysis Pereira, Pedro D.; Serra-Caetano, Ana; Cabrita, Marisa Oncotarget, Vol. 8, Issue 25 https://doi.org/10.18632/oncotarget.17121	journal	April 2017
RanBP2/Nup358 Potentiates the Translation of a Subset of mRNAs Encoding Secretory Proteins Mahadevan, Kohila; Zhang, Hui; Akef, Abdalla PLoS Biology, Vol. 11, Issue 4 https://doi.org/10.1371/journal.pbio.1001545	journal	April 2013
Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene. Heath, C. V.; Copeland, C. S.; Amberg, D. C. Journal of Cell Biology, Vol. 131, Issue 6 https://doi.org/10.1083/jcb.131.6.1677	journal	December 1995
Infection-Triggered Familial or Recurrent Cases of Acute Necrotizing Encephalopathy Caused by Mutations in a Component of the Nuclear Pore, RANBP2 Neilson, Derek E.; Adams, Mark D.; Orr, Caitlin M. D. The American Journal of Human Genetics, Vol. 84, Issue 1 https://doi.org/10.1016/j.ajhg.2008.12.009	journal	January 2009
Variable clinical course in acute necrotizing encephalopathy and identification of a novel RANBP2 mutation Sell, Katharina; Storch, Katja; Hahn, Gabriele Brain and Development, Vol. 38, Issue 8 https://doi.org/10.1016/j.braindev.2016.02.007	journal	September 2016
Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence Bonnin, Edith; Cabochette, Pauline; Filosa, Alessandro PLOS Genetics, Vol. 14, Issue 12 https://doi.org/10.1371/journal.pgen.1007845	journal	December 2018
Cone-shaped HIV-1 capsids are transported through intact nuclear pores Zila, Vojtech; Margiotta, Erica; Turoňová, Beata Cell, Vol. 184, Issue 4 https://doi.org/10.1016/j.cell.2021.01.025	journal	February 2021
The cellular environment shapes the nuclear pore complex architecture Schuller, Anthony P.; Wojtynek, Matthias; Mankus, David Nature, Vol. 598, Issue 7882 https://doi.org/10.1038/s41586-021-03985-3	journal	October 2021
A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex Hamed, Mohamed; Caspar, Birgit; Port, Sarah A. Journal of Cell Science, Vol. 134, Issue 6 https://doi.org/10.1242/jcs.258095	journal	March 2021
The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import Walther, Tobias C.; Pickersgill, Helen S.; Cordes, Volker C. Journal of Cell Biology, Vol. 158, Issue 1 https://doi.org/10.1083/jcb.200202088	journal	July 2002
Natively Unfolded FG Repeats Stabilize the Structure of the Nuclear Pore Complex Onischenko, Evgeny; Tang, Jeffrey H.; Andersen, Kasper R. Cell, Vol. 171, Issue 4 https://doi.org/10.1016/j.cell.2017.09.033	journal	November 2017
SARS-CoV-2 Orf6 hijacks Nup98 to block STAT nuclear import and antagonize interferon signaling Miorin, Lisa; Kehrer, Thomas; Sanchez-Aparicio, Maria Teresa Proceedings of the National Academy of Sciences, Vol. 117, Issue 45 https://doi.org/10.1073/pnas.2016650117	journal	October 2020
Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies Kellner, Nikola; Schwarz, Johannes; Sturm, Miriam Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep20937	journal	February 2016
A Conserved Biogenesis Pathway for Nucleoporins: Proteolytic Processing of a 186-Kilodalton Precursor Generates Nup98 and the Novel Nucleoporin, Nup96 Fontoura, Beatriz M. A.; Blobel, Günter; Matunis, Michael J. The Journal of Cell Biology, Vol. 144, Issue 6 https://doi.org/10.1083/jcb.144.6.1097	journal	March 1999
Interaction between the Shuttling mRNA Export Factor Gle1 and the Nucleoporin hCG1: A Conserved Mechanism in the Export of Hsp70 mRNA Kendirgi, Frederic; Rexer, Deborah J.; Alcázar-Román, Abel R. Molecular Biology of the Cell, Vol. 16, Issue 9 https://doi.org/10.1091/mbc.e04-11-0998	journal	September 2005
Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast Mossessova, Elena; Lima, Christopher D. Molecular Cell, Vol. 5, Issue 5 https://doi.org/10.1016/S1097-2765(00)80326-3	journal	May 2000
Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies Romier, Christophe; Ben Jelloul, Marouane; Albeck, Shira Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 10 https://doi.org/10.1107/S0907444906031003	journal	September 2006
Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins Mukherjee, Somnath; Erramilli, Satchal K.; Ammirati, Mark Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15363-0	journal	March 2020
Preparation of selenomethionyl proteins for phase determination Doublié, Sylvie Methods in Enzymology https://doi.org/10.1016/S0076-6879(97)76075-0 p. 523-530	book	January 1997
Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport Vetter, Ingrid R.; Nowak, Christine; Nishimoto, Takeharu Nature, Vol. 398, Issue 6722 https://doi.org/10.1038/17969	journal	March 1999
Multiangle Light Scattering: The Basic Tool for Macromolecular Characterization Wyatt, Philip J. Instrumentation Science & Technology, Vol. 25, Issue 1 https://doi.org/10.1080/10739149709351443	journal	February 1997
INTS10–INTS13–INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module Sabath, Kevin; Stäubli, Melanie L.; Marti, Sabrina Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-17232-2	journal	July 2020
T Cell Receptor-Like Recognition of Tumor In Vivo by Synthetic Antibody Fragment Miller, Keith R.; Koide, Akiko; Leung, Brenda PLoS ONE, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pone.0043746	journal	August 2012
A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin–Calmodulin Binding Peptide Interaction Mukherjee, Somnath; Ura, Marcin; Hoey, Robert J. Journal of Molecular Biology, Vol. 427, Issue 16 https://doi.org/10.1016/j.jmb.2015.06.018	journal	August 2015
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
DIALS : implementation and evaluation of a new integration package Winter, Graeme; Waterman, David G.; Parkhurst, James M. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 2 https://doi.org/10.1107/S2059798317017235	journal	February 2018
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
A short history of SHELX Sheldrick, George M. Acta Crystallographica Section A Foundations of Crystallography, Vol. 64, Issue 1, p. 112-122 https://doi.org/10.1107/S0108767307043930	journal	December 2007
Substructure search procedures for macromolecular structures Grosse-Kunstleve, R. W.; Adams, P. D. Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 11 https://doi.org/10.1107/S0907444903018043	journal	October 2003
Maximum-likelihood density modification Terwilliger, Thomas C. Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 8 https://doi.org/10.1107/S0907444900005072	journal	August 2000
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Liebschner, Dorothee; Afonine, Pavel V.; Baker, Matthew L. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10 https://doi.org/10.1107/S2059798319011471	journal	October 2019
MolProbity: More and better reference data for improved all-atom structure validation: PROTEIN SCIENCE.ORG Williams, Christopher J.; Headd, Jeffrey J.; Moriarty, Nigel W. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3330	journal	November 2017
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Structural and Functional Analysis of the C-Terminal Domain of Nup358/RanBP2 Lin, Daniel H.; Zimmermann, Stephan; Stuwe, Tobias Journal of Molecular Biology, Vol. 425, Issue 8 https://doi.org/10.1016/j.jmb.2013.01.021	journal	April 2013
Statistical mechanics of chain molecules Flory, Paul. J.; Volkenstein, M. Biopolymers, Vol. 8, Issue 5 https://doi.org/10.1002/bip.1969.360080514	journal	November 1969
Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Linkers Evers, Toon H.; van Dongen, Elisabeth M. W. M.; Faesen, Alex C. Biochemistry, Vol. 45, Issue 44 https://doi.org/10.1021/bi061288t	journal	November 2006
Protein Interfaces of the Conserved Nup84 Complex from Chaetomium thermophilum Shown by Crosslinking Mass Spectrometry and Electron Microscopy Thierbach, Karsten; von Appen, Alexander; Thoms, Matthias Structure, Vol. 21, Issue 9 https://doi.org/10.1016/j.str.2013.07.004	journal	September 2013
Multiplex Genome Engineering Using CRISPR/Cas Systems Cong, L.; Ran, F. A.; Cox, D. Science, Vol. 339, Issue 6121, p. 819-823 https://doi.org/10.1126/science.1231143	journal	January 2013
RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics Yau, Ka Chun; Arnaoutov, Alexei; Aksenova, Vasilisa Cell Cycle, Vol. 19, Issue 15 https://doi.org/10.1080/15384101.2020.1782036	journal	June 2020
Mutation of the Human Circadian Clock Gene CRY1 in Familial Delayed Sleep Phase Disorder Patke, Alina; Murphy, Patricia J.; Onat, Onur Emre Cell, Vol. 169, Issue 2 https://doi.org/10.1016/j.cell.2017.03.027	journal	April 2017
Fiji: an open-source platform for biological-image analysis Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2019	journal	June 2012
Multiple sequence alignment with Clustal X Jeanmougin, François; Thompson, Julie D.; Gouy, Manolo Trends in Biochemical Sciences, Vol. 23, Issue 10 https://doi.org/10.1016/S0968-0004(98)01285-7	journal	October 1998
ALSCRIPT: a tool to format multiple sequence alignments Barton, Geoffrey J. "Protein Engineering, Design and Selection", Vol. 6, Issue 1 https://doi.org/10.1093/protein/6.1.37	journal	January 1993
Electrostatics of nanosystems: Application to microtubules and the ribosome Baker, N. A.; Sept, D.; Joseph, S. Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041 https://doi.org/10.1073/pnas.181342398	journal	August 2001
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. Grandi, P.; Schlaich, N.; Tekotte, H. The EMBO Journal, Vol. 14, Issue 1 https://doi.org/10.1002/j.1460-2075.1995.tb06977.x	journal	January 1995
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences Loughlin, F. E.; Mansfield, R. E.; Vaz, P. M. Proceedings of the National Academy of Sciences, Vol. 106, Issue 14 https://doi.org/10.1073/pnas.0802466106	journal	March 2009
The Solution Structure of FUS Bound to RNA Reveals a Bipartite Mode of RNA Recognition with Both Sequence and Shape Specificity Loughlin, Fionna E.; Lukavsky, Peter J.; Kazeeva, Tamara Molecular Cell, Vol. 73, Issue 3 https://doi.org/10.1016/j.molcel.2018.11.012	journal	February 2019
Dynamic and Equilibrium Studies on the Interaction of Ran with Its Effector, RanBP1 Kuhlmann, Jürgen; Macara, Ian; Wittinghofer, Alfred Biochemistry, Vol. 36, Issue 40 https://doi.org/10.1021/bi970524k	journal	October 1997
JPred4: a protein secondary structure prediction server Drozdetskiy, Alexey; Cole, Christian; Procter, James Nucleic Acids Research, Vol. 43, Issue W1 https://doi.org/10.1093/nar/gkv332	journal	April 2015
Structural and biochemical analyses of the nuclear pore complex component ELYS identify residues responsible for nucleosome binding Kobayashi, Wataru; Takizawa, Yoshimasa; Aihara, Maya Communications Biology, Vol. 2, Issue 1 https://doi.org/10.1038/s42003-019-0385-7	journal	May 2019
ELYS/MEL-28 Chromatin Association Coordinates Nuclear Pore Complex Assembly and Replication Licensing Gillespie, Peter J.; Khoudoli, Guennadi A.; Stewart, Graeme Current Biology, Vol. 17, Issue 19 https://doi.org/10.1016/j.cub.2007.08.041	journal	October 2007
The human Nup107–160 nuclear pore subcomplex contributes to proper kinetochore functions Zuccolo, Michela; Alves, Annabelle; Galy, Vincent The EMBO Journal, Vol. 26, Issue 7 https://doi.org/10.1038/sj.emboj.7601642	journal	March 2007
The Conserved Transmembrane Nucleoporin NDC1 Is Required for Nuclear Pore Complex Assembly in Vertebrate Cells Mansfeld, Jörg; Güttinger, Stephan; Hawryluk-Gara, Lisa A. Molecular Cell, Vol. 22, Issue 1 https://doi.org/10.1016/j.molcel.2006.02.015	journal	April 2006
MEL‐28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly Franz, Cerstin; Walczak, Rudolf; Yavuz, Sevil EMBO reports, Vol. 8, Issue 2 https://doi.org/10.1038/sj.embor.7400889	journal	January 2007
Insight into Structure and Assembly of the Nuclear Pore Complex by Utilizing the Genome of a Eukaryotic Thermophile Amlacher, Stefan; Sarges, Phillip; Flemming, Dirk Cell, Vol. 146, Issue 2 https://doi.org/10.1016/j.cell.2011.06.039	journal	July 2011
Insights into E3 ligase activity revealed by a SUMO–RanGAP1–Ubc9–Nup358 complex Reverter, David; Lima, Christopher D. Nature, Vol. 435, Issue 7042 https://doi.org/10.1038/nature03588	journal	June 2005

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