skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Structural basis for llama nanobody recognition and neutralization of HIV-1 at the CD4-binding site

Journal Article · · Structure

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
DOE - Office Of Science; NIGMS
OSTI ID:
1872591
Journal Information:
Structure, Vol. 30, Issue (6)
Country of Publication:
United States
Language:
ENGLISH

References (68)

PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
Recent developments in the PHENIX software for automated crystallographic structure determination journal November 2003
Quantitative assessment of masking of neutralization epitopes in HIV-1 journal September 2011
Privateer: software for the conformational validation of carbohydrate structures journal November 2015
EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy journal August 2015
Influenza vaccine: The challenge of antigenic drift journal September 2007
Structural Basis of Immune Evasion at the Site of CD4 Attachment on HIV-1 gp120 journal November 2009
Consistent elicitation of cross-clade HIV-neutralizing responses achieved in guinea pigs after fusion peptide priming by repetitive envelope trimer boosting journal April 2019
Structural Survey of Broadly Neutralizing Antibodies Targeting the HIV-1 Env Trimer Delineates Epitope Categories and Characteristics of Recognition journal January 2019
The CCP4 suite programs for protein crystallography journal September 1994
Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies journal November 2021
MOLPROBITY: structure validation and all-atom contact analysis for nucleic acids and their complexes journal July 2004
Increasing the Potency and Breadth of an HIV Antibody by Using Structure-Based Rational Design journal October 2011
Coot model-building tools for molecular graphics journal November 2004
Llama Antibody Fragments with Cross-Subtype Human Immunodeficiency Virus Type 1 (HIV-1)-Neutralizing Properties and High Affinity for HIV-1 gp120 journal October 2008
Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization journal May 2013
A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation journal June 2021
Naturally occurring antibodies devoid of light chains journal June 1993
Crystal Structure of the Neutralizing Llama VHH D7 and Its Mode of HIV-1 gp120 Interaction journal May 2010
Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth journal November 2016
Dendroscope 3: An Interactive Tool for Rooted Phylogenetic Trees and Networks journal September 2012
Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape journal January 2021
Generation of a Family-specific Phage Library of Llama Single Chain Antibody Fragments That Neutralize HIV-1 journal April 2010
Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization journal July 2019
Inference of Macromolecular Assemblies from Crystalline State journal September 2007
A matrix of structure-based designs yields improved VRC01-class antibodies for HIV-1 therapy and prevention journal January 2021
Unliganded HIV-1 gp120 core structures assume the CD4-bound conformation with regulation by quaternary interactions and variable loops journal March 2012
Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env journal June 2015
HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites journal December 2002
Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody journal June 1998
Appion: An integrated, database-driven pipeline to facilitate EM image processing journal April 2009
Universal protection against influenza infection by a multidomain antibody to influenza hemagglutinin journal November 2018
Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer journal March 2016
Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer journal February 2017
The EMBL-EBI search and sequence analysis tools APIs in 2019 journal April 2019
Phaser crystallographic software journal July 2007
Potent and broad neutralization of HIV-1 by a llama antibody elicited by immunization journal May 2012
Molecular Evolution of Broadly Neutralizing Llama Antibodies to the CD4-Binding Site of HIV-1 journal December 2014
[20] Processing of X-ray diffraction data collected in oscillation mode book January 1997
Open and closed structures reveal allostery and pliability in the HIV-1 envelope spike journal July 2017
Vaccine-Induced Protection from Homologous Tier 2 SHIV Challenge in Nonhuman Primates Depends on Serum-Neutralizing Antibody Titers journal January 2019
UCSF Chimera?A visualization system for exploratory research and analysis journal January 2004
UCSF ChimeraX : Structure visualization for researchers, educators, and developers journal October 2020
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination journal February 2017
CTFFIND4: Fast and accurate defocus estimation from electron micrographs journal November 2015
Crystal structure of an HIV-binding recombinant fragment of human CD4 journal November 1990
HIV-1 neutralizing antibodies induced by native-like envelope trimers journal June 2015
Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1 journal July 2014
RELION: Implementation of a Bayesian approach to cryo-EM structure determination journal December 2012
Rapid elicitation of broadly neutralizing antibodies to HIV by immunization in cows journal July 2017
Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G journal May 2016
Llama Antibody Fragments Recognizing Various Epitopes of the CD4bs Neutralize a Broad Range of HIV-1 Subtypes A, B and C journal March 2012
Automated molecular microscopy: The new Leginon system journal July 2005
Characterization of conserved human immunodeficiency virus type 1 gp120 neutralization epitopes exposed upon gp120-CD4 binding. journal January 1993
A Toolbox for ab initio 3-D reconstructions in single-particle electron microscopy journal March 2010
Atomic structure of a fragment of human CD4 containing two immunoglobulin-like domains journal November 1990
MolProbity: More and better reference data for improved all-atom structure validation: PROTEIN SCIENCE.ORG journal November 2017
Epitopes Involved in Antibody-Mediated Protection from Ebola Virus journal March 2000
Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies journal June 2020
Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1 journal July 2010
Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep Sequencing journal August 2011
Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants journal June 2021
Gctf: Real-time CTF determination and correction journal January 2016
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy journal February 2017
Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01 journal July 2010
Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors journal June 2015
Structural definition of a conserved neutralization epitope on HIV-1 gp120 journal February 2007
The Cross-Clade Neutralizing Activity of a Human Monoclonal Antibody Is Determined by the GPGR V3 Motif of HIV Type 1 journal November 2004