skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Rationally designed immunogens enable immune focusing following SARS-CoV-2 spike imprinting

Journal Article · · Cell Reports

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
DOE - Office Of Science; National Institutes of Health (NIH)
OSTI ID:
1863044
Journal Information:
Cell Reports, Vol. 38, Issue (12)
Country of Publication:
United States
Language:
ENGLISH

References (99)

Effectiveness of the BNT162b2 Covid-19 Vaccine against the B.1.1.7 and B.1.351 Variants journal July 2021
PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
SARS-CoV-2 Vaccines: Status Report journal April 2020
Influenza Antigen Engineering Focuses Immune Responses to a Subdominant but Broadly Protective Viral Epitope journal June 2019
Structure-Guided Molecular Grafting of a Complex Broadly Neutralizing Viral Epitope journal April 2020
SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies journal October 2020
Outbreak of SARS-CoV-2 Infections, Including COVID-19 Vaccine Breakthrough Infections, Associated with Large Public Gatherings — Barnstable County, Massachusetts, July 2021 journal August 2021
The time course of the immune response to experimental coronavirus infection of man journal October 1990
MolProbity : all-atom structure validation for macromolecular crystallography journal December 2009
Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies journal March 2021
A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2 journal June 2020
Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice journal February 2021
The CCP4 suite programs for protein crystallography journal September 1994
Sensitivity of SARS-CoV-2 B.1.1.7 to mRNA vaccine-elicited antibodies journal March 2021
Proof of principle for epitope-focused vaccine design journal February 2014
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays journal May 2020
Structure and Immune Recognition of the HIV Glycan Shield journal May 2018
A Universal Design of Betacoronavirus Vaccines against COVID-19, MERS, and SARS journal August 2020
Disquisitions on Original Antigenic sin journal September 1966
Transmission of MERS-Coronavirus in Household Contacts journal August 2014
Infection and Vaccine-Induced Neutralizing-Antibody Responses to the SARS-CoV-2 B.1.617 Variants journal August 2021
Coot model-building tools for molecular graphics journal November 2004
Scaling and assessment of data quality journal December 2005
An introduction to data reduction: space-group determination, scaling and intensity statistics journal March 2011
How good are my data and what is the resolution? journal June 2013
COVID-19-neutralizing antibodies predict disease severity and survival journal January 2021
Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity journal April 2021
Antibodies elicited by mRNA-1273 vaccination bind more broadly to the receptor binding domain than do those from SARS-CoV-2 infection journal June 2021
Studies in humanized mice and convalescent humans yield a SARS-CoV-2 antibody cocktail journal June 2020
A SARS-CoV-2 Infection Model in Mice Demonstrates Protection by Neutralizing Antibodies journal August 2020
Safety Monitoring of an Additional Dose of COVID-19 Vaccine — United States, August 12–September 19, 2021 journal October 2021
Structure-based design of prefusion-stabilized SARS-CoV-2 spikes journal July 2020
SARS‐CoV‐2 RBD Neutralizing Antibody Induction is Enhanced by Particulate Vaccination journal October 2020
Characterization of Influenza Antibodies by Serum Absorption journal August 1956
The neutralizing antibody, LY-CoV555, protects against SARS-CoV-2 infection in nonhuman primates journal May 2021
Human neutralizing antibodies elicited by SARS-CoV-2 infection journal May 2020
Integration, scaling, space-group assignment and post-refinement journal January 2010
XDS journal January 2010
Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19 journal October 2020
Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates journal January 2021
Reconstructing a B-Cell Clonal Lineage. II. Mutation, Selection, and Affinity Maturation journal April 2014
Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor journal March 2020
Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses journal February 2020
Self-Assembling Nanoparticle Vaccines Displaying the Receptor Binding Domain of SARS-CoV-2 Elicit Robust Protective Immune Responses in Rhesus Monkeys journal May 2021
Cross-Neutralization of a SARS-CoV-2 Antibody to a Functionally Conserved Site Is Mediated by Avidity journal December 2020
RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response journal November 2020
Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses journal December 2020
Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice journal August 2021
A broadly cross-reactive antibody neutralizes and protects against sarbecovirus challenge in mice journal January 2022
Automated electron microscope tomography using robust prediction of specimen movements journal October 2005
Phaser crystallographic software journal July 2007
A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence journal November 2015
SARS-like WIV1-CoV poised for human emergence journal March 2016
The Tecumseh Study of Respiratory Illness. VI. Frequency of and Relationship between Outbreaks of Coronavims Infection journal March 1974
Retroviruses Pseudotyped with the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Efficiently Infect Cells Expressing Angiotensin-Converting Enzyme 2 journal October 2004
Effectiveness of Pfizer-BioNTech and Moderna Vaccines in Preventing SARS-CoV-2 Infection Among Nursing Home Residents Before and During Widespread Circulation of the SARS-CoV-2 B.1.617.2 (Delta) Variant — National Healthcare Safety Network, March 1–August 1, 2021 journal August 2021
Elicitation of structure-specific antibodies by epitope scaffolds journal September 2010
UCSF Chimera?A visualization system for exploratory research and analysis journal January 2004
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology journal November 2020
Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody journal May 2020
Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies journal March 2021
Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC journal March 2013
Broad and potent activity against SARS-like viruses by an engineered human monoclonal antibody journal February 2021
Systematic design and testing of nested (RT-)PCR primers for specific amplification of mouse rearranged/expressed immunoglobulin variable region genes from small number of B cells journal December 2008
New COVID-19 Cases and Hospitalizations Among Adults, by Vaccination Status — New York, May 3–July 25, 2021 journal August 2021
Germline-Encoded Affinity for Cognate Antigen Enables Vaccine Amplification of a Human Broadly Neutralizing Response against Influenza Virus journal October 2019
Lessons for COVID-19 Immunity from Other Coronavirus Infections journal August 2020
Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses journal May 2021
RELION: Implementation of a Bayesian approach to cryo-EM structure determination journal December 2012
Immunogenic Stimulus for Germline Precursors of Antibodies that Engage the Influenza Hemagglutinin Receptor-Binding Site journal December 2015
Viral Receptor-Binding Site Antibodies with Diverse Germline Origins journal May 2015
Rises in Titers of Antibody to Human Corona Viruses oc43 and 229e in Seattle Families During 1975–1979 journal May 1986
Structural basis of receptor recognition by SARS-CoV-2 journal March 2020
Glycan engineering of the SARS-CoV-2 receptor-binding domain elicits cross-neutralizing antibodies for SARS-related viruses journal October 2021
Affordable Luciferase Reporter Assay for Cell-Based High-Throughput Screening journal April 2013
Immunosilencing a Highly Immunogenic Protein Trimerization Domain journal January 2015
Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera journal April 2021
Neutralizing and protective human monoclonal antibodies recognizing the N-terminal domain of the SARS-CoV-2 spike protein journal April 2021
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard journal December 2007
Cloning and expression of murine Ig genes from single B cells journal October 2009
Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike journal September 2021
Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2 journal November 2020
Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccines journal October 2021
Ferritin nanoparticle-based SARS-CoV-2 RBD vaccine induces a persistent antibody response and long-term memory in mice journal February 2021
mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants journal February 2021
In vitro reconstitution of B cell receptor–antigen interactions to evaluate potential vaccine candidates journal January 2016
Broad neutralization of SARS-related viruses by human monoclonal antibodies journal June 2020
SARS-CoV-2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma journal March 2021
SARS-CoV-2 infection of human ACE2-transgenic mice causes severe lung inflammation and impaired function journal August 2020
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation journal February 2020
Serum Neutralizing Activity Elicited by mRNA-1273 Vaccine journal April 2021
Duration of Antibody Responses after Severe Acute Respiratory Syndrome journal October 2007
A natural mutation between SARS-CoV-2 and SARS-CoV determines neutralization by a cross-reactive antibody journal December 2020
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2 journal May 2020
A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity journal July 2020
Structural and functional ramifications of antigenic drift in recent SARS-CoV-2 variants journal August 2021
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV journal April 2020
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin journal February 2012
Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera journal April 2021