The first intron of human c-fms proto-oncogene contains a processed pseudogene (RPL7P) for ribosomal protein L7
- Yale Univ. School of Medicine, New Haven, CT (United States)
During sequence analysis of the first intron of the human c-fms oncogene, we identified an open reading frame encoding the ribosomal protein L7 (RPL7). The presence of this sequence within intron 1 of the c-fms gene was confirmed by Southern blot hybridization and by sequence analysis of two independent cosmid clones (cos2-e and cos1-22) that span the human genomic c-fms locus. The RPL7 sequence was detected in a region of sequence overlapped by the cos2-e and cos1-22 cosmid clones but oriented opposite to the c-fms gene. We demonstrate that the sequence is identical to the full-length RPL7 cDNA sequence, but lacks any recognizable introns, has a 30-bp poly(A) tail, and is bracketed by two perfect direct repeats of 14 bp. We also showed that despite the fact that the 5{prime} flanking region of the RPL7 sequence contains a potential TATA box upstream of an intact open reading frame, this pseudogene (RPL7P) is not actively transcribed. 28 refs., 4 figs.
- OSTI ID:
- 186037
- Journal Information:
- Genomics, Vol. 22, Issue 3; Other Information: PBD: Aug 1994
- Country of Publication:
- United States
- Language:
- English
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