Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Methods for producing microcompartments

Patent ·
OSTI ID:1860163
; ; ; ;

To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Oakland, CA (United States)
Sponsoring Organization:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); National Science Foundation (NSF)
DOE Contract Number:
AC02-05CH11231
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (Oakland, CA)
Patent Number(s):
11,198,880
Application Number:
15/367,089
OSTI ID:
1860163
Country of Publication:
United States
Language:
English

References (20)

Engineered Protein Nano-Compartments for Targeted Enzyme Localization journal March 2012
Automated design of synthetic ribosome binding sites to control protein expression journal October 2009
Carboxysomes and Carboxysome-like Inclusions book January 2006
Protein Content of Polyhedral Organelles Involved in Coenzyme B12-Dependent Degradation of 1,2-Propanediol in Salmonella enterica Serovar Typhimurium LT2 journal August 2003
Bacterial microcompartments moving into a synthetic biological world journal January 2013
Atomic-Level Models of the Bacterial Carboxysome Shell journal February 2008
Bacterial microcompartments: their properties and paradoxes journal November 2008
Four Novel Genes Required for Optimal Photoautotrophic Growth of the Cyanobacterium Synechocystis sp. Strain PCC 6803 Identified by In Vitro Transposon Mutagenesis journal January 2004
Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement journal April 2010
Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis journal March 2008
Protein-based organelles in bacteria: carboxysomes and related microcompartments journal August 2008
Bacterial Microcompartments journal October 2010
Polyhedral organelles compartmenting bacterial metabolic processes journal March 2006
Assembly of Robust Bacterial Microcompartment Shells Using Building Blocks from an Organelle of Unknown Function journal May 2014
Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies journal September 2006
Short N-terminal sequences package proteins into bacterial microcompartments journal March 2010
Protein Structures Forming the Shell of Primitive Bacterial Organelles journal August 2005
The 5'-flanking region of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is crucial for growth of the cyanobacterium Synechococcus sp. strain PCC 7942 at the level of CO2 in air journal November 1989
Construction of a Synechocystic PCC6803 mutant suitable for the study of variant hexadecameric ribulose bisphosphate carboxylase/oxygenase enzymes journal November 1993
A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method journal October 2014

Similar Records

Structural Characterization of a Synthetic Tandem-Domain Bacterial Microcompartment Shell Protein Capable of Forming Icosahedral Shell Assemblies
Journal Article · 2019 · ACS Synthetic Biology · OSTI ID:1671311
Bacterial microcompartments: tiny organelles with big potential
Journal Article · 2021 · Current Opinion in Microbiology · OSTI ID:1977446

Related Subjects