Insights into community of photosynthetic microorganisms from permafrost

Vishnivetskaya, Tatiana A.; Almatari, Abraham L.; Spirina, Elena V.; Wu, Xiaofen; Williams, Daniel E.; Pfiffner, Susan M.; Rivkina, Elizaveta M.

doi:10.1093/femsec/fiaa229

Insights into community of photosynthetic microorganisms from permafrost

Journal Article · Sun Nov 01 04:00:00 UTC 2020 · FEMS Microbiology Ecology

DOI:https://doi.org/10.1093/femsec/fiaa229· OSTI ID:1853610

^[1]; Almatari, Abraham L. ^[2]; Spirina, Elena V. ^[3]; Wu, Xiaofen ^[2]; Williams, Daniel E. ^[2]; Pfiffner, Susan M. ^[2]; ^[3]

Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, 1416 Circle Drive, Knoxville, TN 37996-1605, USA; Soil Cryology Laboratory, Institute of Physicochemical and Biological Problems in Soil Science, Institutskaya Street, Bldg. 2, Pushchino, Russia; OSTI
Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, 1416 Circle Drive, Knoxville, TN 37996-1605, USA
Soil Cryology Laboratory, Institute of Physicochemical and Biological Problems in Soil Science, Institutskaya Street, Bldg. 2, Pushchino, Russia

ABSTRACT

This work integrates cultivation studies of Siberian permafrost and analyses of metagenomes from different locations in the Arctic with the aim of obtaining insights into the community of photosynthetic microorganisms in perennially frozen deposits. Cyanobacteria and microalgae have been described in Arctic aquatic and surface soil environments, but their diversity and ability to withstand harsh conditions within the permafrost are still largely unknown. Community structure of photosynthetic organisms in permafrost sediments was explored using Arctic metagenomes available through the MG-RAST. Sequences affiliated with cyanobacteria represented from 0.25 to 3.03% of total sequences, followed by sequences affiliated with Streptophyta (algae and vascular plants) 0.01–0.45% and Chlorophyta (green algae) 0.01–0.1%. Enrichment and cultivation approaches revealed that cyanobacteria and green algae survive in permafrost and they could be revived during prolonged incubation at low light intensity. Among photosynthetic microorganisms isolated from permafrost, the filamentous Oscillatoria-like cyanobacteria and unicellular green algae of the genus Chlorella were dominant. Our findings suggest that permafrost cyanobacteria and green algae are expected to be effective members of the re-assembled community after permafrost thawing and soil collapse.

Research Organization:: Univ. of Tennessee, Knoxville, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0020369

OSTI ID:: 1853610

Journal Information:: FEMS Microbiology Ecology, Journal Name: FEMS Microbiology Ecology Journal Issue: 12 Vol. 96; ISSN 0168-6496

Publisher:: Federation of European Microbiology Societies

Country of Publication:: United States

Language:: English

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