Hybrid radical-polar pathway for excision of ethylene from 2-oxoglutarate by an iron oxygenase

Copeland, Rachelle A.; Zhou, Shengbin; Schaperdoth, Irene; Shoda, Tokufu Kent C.; Bollinger, J. Martin; Krebs, Carsten

doi:10.1126/science.abj4290

Hybrid radical-polar pathway for excision of ethylene from 2-oxoglutarate by an iron oxygenase

Journal Article · 24 September 2021 · Science

DOI:https://doi.org/10.1126/science.abj4290· OSTI ID:1852166

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Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.; OSTI
Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.
Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.

Tracing a path to ethylene

Ethylene is produced industrially from fossil carbon sources, but plants and microbes produce small amounts through a handful of unusual enzymatic reactions. Copelandet al. studied a microbial ethylene-forming enzyme using oxygen isotope–tracing experiments and biochemical assays to test mechanistic proposals. This enzyme, which uses a non–heme iron center to activate oxygen, catalyzes two distinct oxidation reactions with different mechanisms. One reaction is entirely off-pathway and results in fragmentation of a co-substrate, arginine. The other reaction can completely fragment 2-oxoglutarate into ethylene, bicarbonate, and two molecules of carbon dioxide; however, it occasionally derails and yields an omega–hydroxy acid product. —MAF

Research Organization:: Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0016255;

OSTI ID:: 1852166

Journal Information:: Science, Journal Name: Science Journal Issue: 6562 Vol. 373; ISSN 0036-8075

Publisher:: AAAS

Country of Publication:: United States

Language:: English

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