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The Cell Utilized Partitioning Model as a Predictive Tool for Optimizing Counter-Current Chromatography Processes

Journal Article · · Separation and Purification Technology

Counter-current chromatography (CCC) is capable of unique elution modes that isolate analytes using the movement of the stationary phase in addition to moving the mobile phase. These modes include elution-extrusion CCC (EECCC) and dual-mode CCC (DM CCC) that are not possible in traditional solid-liquid chromatography systems. Although EECCC and DM CCC are widely used to recover highly retained components, to our knowledge, optimizing the elution process in these modes with predictive models has not been reported. To address this gap, we developed a predictive model for CCC dubbed the Cell Utilized Partitioning (CUP) model. The CUP model accurately predicts the effluents of multicomponent separations in EECCC and DM CCC modes when compared to experimental data. Furthermore, CUP model simulations were extended to investigate the influence of operating and intrinsic parameters on the yield and productivity, and to compare the separation performances of EECCC and DM CCC in various conditions. The results demonstrate that low distribution constants, usually a KD less than 1, and a selectivity > 1.3, under specific flowrate ranges, increase both productivity and yield. From these results, generalized optimization and scaleup guidelines are proposed that can apply to research settings and to industrial processes to maximize preparative CCC performance.

Research Organization:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
DOE Contract Number:
AC36-08GO28308
OSTI ID:
1847265
Report Number(s):
NREL/JA-2800-81788; MainId:82561; UUID:100ebabb-218f-4248-a167-958e5d75513b; MainAdminID:63959
Journal Information:
Separation and Purification Technology, Vol. 285
Country of Publication:
United States
Language:
English

References (27)

Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography journal February 2005
Elution−Extrusion Countercurrent Chromatography. Use of the Liquid Nature of the Stationary Phase To Extend the Hydrophobicity Window journal September 2003
Operating mode and parameter selection in liquid–liquid chromatography journal April 2020
Elution−Extrusion Countercurrent Chromatography:  Theory and Concepts in Metabolic Analysis journal April 2007
Isolation of β-carotene, α-carotene and lutein from carrots by countercurrent chromatography with the solvent system modifier benzotrifluoride journal April 2015
Using the liquid nature of the stationary phase. VI. Theoretical study of multi-dual mode countercurrent chromatography journal September 2011
Separation and purification of lanosterol, dihydrolanosterol, and cholesterol from lanolin by high‐performance counter‐current chromatography dual‐mode elution method journal April 2019
A novel approach to modelling counter-current chromatography journal October 2010
Modelling CCC Using an Eluting Countercurrent Distribution Model journal June 2003
Universal counter-current chromatography modelling based on counter-current distribution journal May 2009
Modelling counter-current chromatography: a chemical engineering perspective journal October 2002
Modeling counter-current chromatography with non-ideal injection journal June 2020
Modelling counter-current chromatography using a temperature dependence plate model journal August 2013
Modeling of closed-loop recycling liquid–liquid chromatography: Analytical solutions and model analysis journal August 2015
Modelling counter-current and dual counter-current chromatography using longitudinal mixing cell and eluting counter-current distribution models journal June 2007
Modelling of elution–extrusion counter-current chromatography using perfect replacement approach journal September 2011
An easy-to-use calculating machine to simulate steady state and non-steady-state preparative separations by multiple dual mode counter-current chromatography with semi-continuous loading of feed mixtures journal June 2018
Theoretical study of sequential centrifugal partition chromatography journal April 2012
Apparatus for Countercurrent Distribution journal April 1949
Key parameters controlling the development of constant-pattern isotachic trains of two rare earth elements in ligand-assisted displacement chromatography journal August 2018
Flow-through solvolysis enables production of native-like lignin from biomass journal January 2021
Stationary Phase Retention in CCC: Modelling the J‐Type Centrifuge as a Constant Pressure Drop Pump journal June 2003
Use of Limonene in Countercurrent Chromatography: A Green Alkane Substitute journal April 2013
Comparing the optimum performance of the different modes of preparative liquid chromatography journal February 1998
Overlapping elution–extrusion counter-current chromatography: A novel method for efficient purification of natural cytotoxic andrographolides from Andrographis paniculata journal February 2012
Simulated moving bed multiobjective optimization using standing wave design and genetic algorithm journal November 2008
Comparison of volume and concentration overloadings in preparative enantio-separations by supercritical fluid chromatography journal May 2014