Directed evolution of and structural insights into antibody-mediated disruption of a stable receptor

Pennington, Luke F.; Gasser, Pascal; Kleinboelting, Silke; Zhang, Chensong; Skiniotis, Georgios; Eggel, Alexander; Jardetzky, Theodore S.

doi:10.1038/s41467-021-27397-z

Directed evolution of and structural insights into antibody-mediated disruption of a stable receptor

Journal Article · 01 December 2021 · Nat. Commun.

DOI:https://doi.org/10.1038/s41467-021-27397-z· OSTI ID:1839704

Pennington, Luke F.; Gasser, Pascal; Kleinboelting, Silke; Zhang, Chensong; Skiniotis, Georgios; Eggel, Alexander; Jardetzky, Theodore S.

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: DOE - Office Of Science; FOREIGN; National Institutes of Health (NIH)

OSTI ID:: 1839704

Journal Information:: Nat. Commun., Vol. 12

Country of Publication:: United States

Language:: ENGLISH

References (43)

Therapeutic Antibodies: What Have We Learnt from Targeting CD20 and Where Are We Going? Marshall, Michael J. E.; Stopforth, Richard J.; Cragg, Mark S. Frontiers in Immunology, Vol. 8 https://doi.org/10.3389/fimmu.2017.01245	journal	October 2017
Therapeutic antibodies: their mechanisms of action and the pathological findings they induce in toxicity studies Suzuki, Masami; Kato, Chie; Kato, Atsuhiko Journal of Toxicologic Pathology, Vol. 28, Issue 3 https://doi.org/10.1293/tox.2015-0031	journal	January 2015
Accelerated disassembly of IgE–receptor complexes by a disruptive macromolecular inhibitor Kim, Beomkyu; Eggel, Alexander; Tarchevskaya, Svetlana S. Nature, Vol. 491, Issue 7425 https://doi.org/10.1038/nature11546	journal	October 2012
Accelerated dissociation of IgE-FcεRI complexes by disruptive inhibitors actively desensitizes allergic effector cells Eggel, Alexander; Baravalle, Günther; Hobi, Gabriel Journal of Allergy and Clinical Immunology, Vol. 133, Issue 6 https://doi.org/10.1016/j.jaci.2014.02.005	journal	June 2014
Discovery of high affinity, pan-allelic, and pan-mammalian reactive antibodies against the myeloid checkpoint receptor SIRPα Sim, Janet; Sockolosky, Jonathan T.; Sangalang, Emma mAbs, Vol. 11, Issue 6 https://doi.org/10.1080/19420862.2019.1624123	journal	June 2019
Antibodies Targeting Closely Adjacent or Minimally Overlapping Epitopes Can Displace One Another Abdiche, Yasmina Noubia; Yeung, Andy Yik; Ni, Irene PLOS ONE, Vol. 12, Issue 1 https://doi.org/10.1371/journal.pone.0169535	journal	January 2017
Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange Pennington, Luke F.; Tarchevskaya, Svetlana; Brigger, Daniel Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms11610	journal	May 2016
The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab Gasser, Pascal; Tarchevskaya, Svetlana S.; Guntern, Pascal Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-019-13815-w	journal	January 2020
Omalizumab facilitates rapid oral desensitization for peanut allergy MacGinnitie, Andrew J.; Rachid, Rima; Gragg, Hana Journal of Allergy and Clinical Immunology, Vol. 139, Issue 3 https://doi.org/10.1016/j.jaci.2016.08.010	journal	March 2017
Ligelizumab for Chronic Spontaneous Urticaria Maurer, Marcus; Giménez-Arnau, Ana M.; Sussman, Gordon New England Journal of Medicine, Vol. 381, Issue 14 https://doi.org/10.1056/NEJMoa1900408	journal	October 2019
Treatment of Allergic Asthma with Monoclonal Anti-IgE Antibody Milgrom, Henry; Fick, Robert B.; Su, John Q. New England Journal of Medicine, Vol. 341, Issue 26 https://doi.org/10.1056/NEJM199912233412603	journal	December 1999
Long Term Maintenance of IgE-Mediated Memory in Mast Cells in the Absence of Detectable Serum IgE Kubo, Shuichi; Nakayama, Toshinori; Matsuoka, Kunie The Journal of Immunology, Vol. 170, Issue 2 https://doi.org/10.4049/jimmunol.170.2.775	journal	January 2003
The use of omalizumab in the treatment of severe allergic asthma: A clinical experience update Holgate, Stephen; Buhl, Roland; Bousquet, Jean Respiratory Medicine, Vol. 103, Issue 8 https://doi.org/10.1016/j.rmed.2009.03.008	journal	August 2009
Structure-guided design of ultrapotent disruptive IgE inhibitors to rapidly terminate acute allergic reactions Pennington, Luke F.; Gasser, Pascal; Brigger, Daniel Journal of Allergy and Clinical Immunology, Vol. 148, Issue 4 https://doi.org/10.1016/j.jaci.2021.03.050	journal	October 2021
Allosteric mechanism of action of the therapeutic anti-IgE antibody omalizumab Davies, Anna M.; Allan, Elizabeth G.; Keeble, Anthony H. Journal of Biological Chemistry, Vol. 292, Issue 24 https://doi.org/10.1074/jbc.M117.776476	journal	April 2017
Engineering the Fab fragment of the anti-IgE omalizumab to prevent Fab crystallization and permit IgE-Fc complex crystallization Mitropoulou, Alkistis N.; Ceska, Tom; Heads, James T. Acta Crystallographica Section F Structural Biology Communications, Vol. 76, Issue 3 https://doi.org/10.1107/S2053230X20001466	journal	March 2020
Structure-based engineering to restore high affinity binding of an isoform-selective anti-TGFβ1 antibody Lord, Dana M.; Bird, Julie J.; Honey, Denise M. mAbs, Vol. 10, Issue 3 https://doi.org/10.1080/19420862.2018.1426421	journal	February 2018
An Engineered Disulfide Bond Reversibly Traps the IgE-Fc _3–4 in a Closed, Nonreceptor Binding Conformation Wurzburg, Beth A.; Kim, Beomkyu; Tarchevskaya, Svetlana S. Journal of Biological Chemistry, Vol. 287, Issue 43 https://doi.org/10.1074/jbc.M112.407502	journal	September 2012
Molecular evolution by staggered extension process (StEP) in vitro recombination Zhao, Huimin; Giver, Lori; Shao, Zhixin Nature Biotechnology, Vol. 16, Issue 3 https://doi.org/10.1038/nbt0398-258	journal	March 1998
Effect of antigen binding affinity and effector function on the pharmacokinetics and pharmacodynamics of anti-IgE monoclonal antibodies Mortensen, Deborah L.; Prabhu, Saileta; Stefanich, Eric G. mAbs, Vol. 4, Issue 6 https://doi.org/10.4161/mabs.22216	journal	November 2012
Conformational Flexibility in Immunoglobulin E-Fc3–4 Revealed in Multiple Crystal Forms Wurzburg, Beth A.; Jardetzky, Theodore S. Journal of Molecular Biology, Vol. 393, Issue 1 https://doi.org/10.1016/j.jmb.2009.08.012	journal	October 2009
Conformational changes in IgE contribute to its uniquely slow dissociation rate from receptor FcɛRI Holdom, Mary D.; Davies, Anna M.; Nettleship, Joanne E. Nature Structural & Molecular Biology, Vol. 18, Issue 5 https://doi.org/10.1038/nsmb.2044	journal	April 2011
Trapping IgE in a closed conformation by mimicking CD23 binding prevents and disrupts FcεRI interaction Jabs, Frederic; Plum, Melanie; Laursen, Nick S. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-017-02312-7	journal	January 2018
Crystal structure of IgE bound to its B-cell receptor CD23 reveals a mechanism of reciprocal allosteric inhibition with high affinity receptor Fc RI Dhaliwal, B.; Yuan, D.; Pang, M. O. Y. Proceedings of the National Academy of Sciences, Vol. 109, Issue 31 https://doi.org/10.1073/pnas.1207278109	journal	July 2012
Disulfide by Design 2.0: a web-based tool for disulfide engineering in proteins Craig, Douglas B.; Dombkowski, Alan A. BMC Bioinformatics, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2105-14-346	journal	December 2013
Use of Quantitative Pharmacology in the Development of HAE1, a High-Affinity Anti-IgE Monoclonal Antibody Putnam, Wendy S.; Li, Jing; Haggstrom, Jonas The AAPS Journal, Vol. 10, Issue 2 https://doi.org/10.1208/s12248-008-9045-4	journal	June 2008
Novel Structural Determinants on SIRPα that Mediate Binding to CD47 Lee, Winston Y.; Weber, Dominique A.; Laur, Oskar The Journal of Immunology, Vol. 179, Issue 11 https://doi.org/10.4049/jimmunol.179.11.7741	journal	November 2007
Pharmacokinetics, pharmacodynamics and safety of QGE031 (ligelizumab), a novel high-affinity anti-IgE antibody, in atopic subjects Arm, J. P.; Bottoli, I.; Skerjanec, A. Clinical & Experimental Allergy, Vol. 44, Issue 11 https://doi.org/10.1111/cea.12400	journal	October 2014
Monitoring free serum IgE in severe asthma patients treated with omalizumab Korn, Stephanie; Haasler, Ina; Fliedner, Florian Respiratory Medicine, Vol. 106, Issue 11 https://doi.org/10.1016/j.rmed.2012.07.010	journal	November 2012
Relationship between omalizumab pharmacokinetics, IgE pharmacodynamics and symptoms in patients with severe persistent allergic (IgE-mediated) asthma Lowe, Philip J.; Tannenbaum, Stacey; Gautier, Aurelie British Journal of Clinical Pharmacology, Vol. 68, Issue 1 https://doi.org/10.1111/j.1365-2125.2009.03401.x	journal	July 2009
High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells Durocher, Y. Nucleic Acids Research, Vol. 30, Issue 2 https://doi.org/10.1093/nar/30.2.e9	journal	January 2002
Kinetic Titration Series with Biolayer Interferometry Frenzel, Daniel; Willbold, Dieter PLoS ONE, Vol. 9, Issue 9 https://doi.org/10.1371/journal.pone.0106882	journal	September 2014
Analyzing a kinetic titration series using affinity biosensors Karlsson, Robert; Katsamba, Phinikoula S.; Nordin, Helena Analytical Biochemistry, Vol. 349, Issue 1 https://doi.org/10.1016/j.ab.2005.09.034	journal	February 2006
Integration, scaling, space-group assignment and post-refinement Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 133-144 https://doi.org/10.1107/S0907444909047374	journal	January 2010
Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Liebschner, Dorothee; Afonine, Pavel V.; Baker, Matthew L. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10 https://doi.org/10.1107/S2059798319011471	journal	October 2019
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Current developments in Coot for macromolecular model building of Electron Cryo‐microscopy and Crystallographic Data Casañal, Ana; Lohkamp, Bernhard; Emsley, Paul Protein Science, Vol. 29, Issue 4 https://doi.org/10.1002/pro.3791	journal	March 2020
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Automated electron microscope tomography using robust prediction of specimen movements Mastronarde, David N. Journal of Structural Biology, Vol. 152, Issue 1 https://doi.org/10.1016/j.jsb.2005.07.007	journal	October 2005
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4193	journal	February 2017
SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM Wagner, Thorsten; Merino, Felipe; Stabrin, Markus Communications Biology, Vol. 2, Issue 1 https://doi.org/10.1038/s42003-019-0437-z	journal	June 2019
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination Punjani, Ali; Rubinstein, John L.; Fleet, David J. Nature Methods, Vol. 14, Issue 3 https://doi.org/10.1038/nmeth.4169	journal	February 2017
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004

Similar Records

Directed evolution of and structural insights into antibody-mediated disruption of a stable receptor-ligand complex

Journal Article · 2021 · Nature Communications · OSTI ID:1841239

Pennington, Luke F.; Gasser, Pascal; Kleinboelting, Silke; +4 more

Disrupted AMPA Receptor Function upon Genetic- or Antibody-Mediated Loss of Autism-Associated CASPR2

Journal Article · 2019 · Cerebral Cortex · OSTI ID:1617260

Fernandes, Dominique; Santos, Sandra D.; Coutinho, Ester; +7 more

Directed evolution of and structural insights into antibody-mediated disruption of a stable receptor

Citation Formats

References (43)

Linked Research (1)

Similar Records

Related Subjects