Improved epitope resolution of the prefusion trimer-specific antibody AM14 bound to the RSV F glycop

Harshbarger, Wayne; Abeyrathne, Priyanka D.; Tian, Sai; Huang, Ying; Chandramouli, Sumana; Bottomley, Matthew James; Malito, Enrico

doi:10.1080/19420862.2021.1955812

Improved epitope resolution of the prefusion trimer-specific antibody AM14 bound to the RSV F glycop

Journal Article · 01 August 2021 · MABS-Austin

DOI:https://doi.org/10.1080/19420862.2021.1955812· OSTI ID:1833702

Harshbarger, Wayne; Abeyrathne, Priyanka D.; Tian, Sai; Huang, Ying; Chandramouli, Sumana; Bottomley, Matthew James; Malito, Enrico

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: DOE - Office Of Science; INDUSTRY

OSTI ID:: 1833702

Journal Information:: MABS-Austin, Vol. 13, Issue (1)

Country of Publication:: United States

Language:: ENGLISH

References (63)

Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis Nair, Harish; Nokes, D. James; Gessner, Bradford D. The Lancet, Vol. 375, Issue 9725 https://doi.org/10.1016/S0140-6736(10)60206-1	journal	May 2010
Burden of respiratory syncytial virus infection in young children Resch, Bernhard World Journal of Clinical Pediatrics, Vol. 1, Issue 3 https://doi.org/10.5409/wjcp.v1.i3.8	journal	January 2012
Strategic priorities for respiratory syncytial virus (RSV) vaccine development Anderson, L. J.; Dormitzer, P. R.; Nokes, D. J. Vaccine, Vol. 31 https://doi.org/10.1016/j.vaccine.2012.11.106	journal	April 2013
Advances in RSV vaccine research and development – A global agenda Higgins, Deborah; Trujillo, Carrie; Keech, Cheryl Vaccine, Vol. 34, Issue 26 https://doi.org/10.1016/j.vaccine.2016.03.109	journal	June 2016
Clinical Potential of Prefusion RSV F-specific Antibodies Rossey, Iebe; McLellan, Jason S.; Saelens, Xavier Trends in Microbiology, Vol. 26, Issue 3 https://doi.org/10.1016/j.tim.2017.09.009	journal	March 2018
Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody McLellan, J. S.; Chen, M.; Leung, S. Science, Vol. 340, Issue 6136 https://doi.org/10.1126/science.1234914	journal	April 2013
Vaccines against human respiratory syncytial virus in clinical trials, where are we now? Rossey, Iebe; Saelens, Xavier Expert Review of Vaccines, Vol. 18, Issue 10 https://doi.org/10.1080/14760584.2019.1675520	journal	October 2019
Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus McLellan, J. S.; Chen, M.; Joyce, M. G. Science, Vol. 342, Issue 6158 https://doi.org/10.1126/science.1243283	journal	October 2013
An Adjuvanted, Postfusion F Protein–Based Vaccine Did Not Prevent Respiratory Syncytial Virus Illness in Older Adults Falloon, Judith; Yu, Jing; Esser, Mark T. The Journal of Infectious Diseases, Vol. 216, Issue 11 https://doi.org/10.1093/infdis/jix503	journal	September 2017
A proof of concept for structure-based vaccine design targeting RSV in humans Crank, Michelle C.; Ruckwardt, Tracy J.; Chen, Man Science, Vol. 365, Issue 6452 https://doi.org/10.1126/science.aav9033	journal	August 2019
Prefusion F–specific antibodies determine the magnitude of RSV neutralizing activity in human sera Ngwuta, Joan O.; Chen, Man; Modjarrad, Kayvon Science Translational Medicine, Vol. 7, Issue 309 https://doi.org/10.1126/scitranslmed.aac4241	journal	October 2015
Electron Microscopy of the Human Respiratory Syncytial Virus Fusion Protein and Complexes That It Forms with Monoclonal Antibodies Calder, Leslie J.; González-Reyes, Luis; García-Barreno, Blanca Virology, Vol. 271, Issue 1 https://doi.org/10.1006/viro.2000.0279	journal	May 2000
Neutralization of Human Respiratory Syncytial Virus Infectivity by Antibodies and Low-Molecular-Weight Compounds Targeted against the Fusion Glycoprotein Magro, Margarita; Andreu, David; Gómez-Puertas, Paulino Journal of Virology, Vol. 84, Issue 16 https://doi.org/10.1128/JVI.00447-10	journal	June 2010
Neutralizing epitopes on the respiratory syncytial virus fusion glycoprotein McLellan, Jason S. Current Opinion in Virology, Vol. 11 https://doi.org/10.1016/j.coviro.2015.03.002	journal	April 2015
Protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies Taylor, G.; Stott, E. J.; Furze, J. Journal of General Virology, Vol. 73, Issue 9 https://doi.org/10.1099/0022-1317-73-9-2217	journal	September 1992
Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors Gilman, M. S. A.; Castellanos, C. A.; Chen, M. Science Immunology, Vol. 1, Issue 6 https://doi.org/10.1126/sciimmunol.aaj1879	journal	December 2016
Immunological goals for respiratory syncytial virus vaccine development Graham, Barney S. Current Opinion in Immunology, Vol. 59 https://doi.org/10.1016/j.coi.2019.03.005	journal	August 2019
Generation of stable monoclonal antibody–producing B cell receptor–positive human memory B cells by genetic programming Kwakkenbos, Mark J.; Diehl, Sean A.; Yasuda, Etsuko Nature Medicine, Vol. 16, Issue 1 https://doi.org/10.1038/nm.2071	journal	December 2009
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein Gilman, Morgan S. A.; Moin, Syed M.; Mas, Vicente PLOS Pathogens, Vol. 11, Issue 7 https://doi.org/10.1371/journal.ppat.1005035	journal	July 2015
Protein Crystallography in Vaccine Research and Development Malito, Enrico; Carfi, Andrea; Bottomley, Matthew International Journal of Molecular Sciences, Vol. 16, Issue 12 https://doi.org/10.3390/ijms160613106	journal	June 2015
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
IMGT/LIGM-DB, the IMGT(R) comprehensive database of immunoglobulin and T cell receptor nucleotide sequences Giudicelli, V. Nucleic Acids Research, Vol. 34, Issue 90001 https://doi.org/10.1093/nar/gkj088	journal	January 2006
Convergent structural features of respiratory syncytial virus neutralizing antibodies and plasticity of the site V epitope on prefusion F Harshbarger, Wayne; Tian, Sai; Wahome, Newton PLOS Pathogens, Vol. 16, Issue 11 https://doi.org/10.1371/journal.ppat.1008943	journal	November 2020
Structure of a Major Antigenic Site on the Respiratory Syncytial Virus Fusion Glycoprotein in Complex with Neutralizing Antibody 101F McLellan, J. S.; Chen, M.; Chang, J. -S. Journal of Virology, Vol. 84, Issue 23 https://doi.org/10.1128/JVI.01579-10	journal	September 2010
Antibody Epitopes of Pneumovirus Fusion Proteins Huang, Jiachen; Diaz, Darren; Mousa, Jarrod J. Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.02778	journal	November 2019
Characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus Arbiza, J.; Taylor, G.; Lopez, J. A. Journal of General Virology, Vol. 73, Issue 9 https://doi.org/10.1099/0022-1317-73-9-2225	journal	September 1992
Antigenic Structure of Human Respiratory Syncytial Virus Fusion Glycoprotein López, Juan A.; Bustos, Regla; Örvell, Claes Journal of Virology, Vol. 72, Issue 8 https://doi.org/10.1128/JVI.72.8.6922-6928.1998	journal	August 1998
Conformational Plasticity in the HIV-1 Fusion Peptide Facilitates Recognition by Broadly Neutralizing Antibodies Yuan, Meng; Cottrell, Christopher A.; Ozorowski, Gabriel Cell Host & Microbe, Vol. 25, Issue 6 https://doi.org/10.1016/j.chom.2019.04.011	journal	June 2019
Structural basis of broad HIV neutralization by a vaccine-induced cow antibody Stanfield, Robyn L.; Berndsen, Zachary T.; Huang, Ruiqi Science Advances, Vol. 6, Issue 22 https://doi.org/10.1126/sciadv.aba0468	journal	May 2020
HIV-1 Envelope and MPER Antibody Structures in Lipid Assemblies Rantalainen, Kimmo; Berndsen, Zachary T.; Antanasijevic, Aleksandar Cell Reports, Vol. 31, Issue 4 https://doi.org/10.1016/j.celrep.2020.107583	journal	April 2020
Cryo-EM Structure of Full-length HIV-1 Env Bound With the Fab of Antibody PG16 Pan, Junhua; Peng, Hanqin; Chen, Bing Journal of Molecular Biology, Vol. 432, Issue 4 https://doi.org/10.1016/j.jmb.2019.11.028	journal	February 2020
Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy Bartesaghi, Alberto; Merk, Alan; Borgnia, Mario J. Nature Structural & Molecular Biology, Vol. 20, Issue 12 https://doi.org/10.1038/nsmb.2711	journal	October 2013
Structural Basis of Protection against H7N9 Influenza Virus by Human Anti-N9 Neuraminidase Antibodies Zhu, Xueyong; Turner, Hannah L.; Lang, Shanshan Cell Host & Microbe, Vol. 26, Issue 6 https://doi.org/10.1016/j.chom.2019.10.002	journal	December 2019
Potent anti-influenza H7 human monoclonal antibody induces separation of hemagglutinin receptor-binding head domains Turner, Hannah L.; Pallesen, Jesper; Lang, Shanshan PLOS Biology, Vol. 17, Issue 2 https://doi.org/10.1371/journal.pbio.3000139	journal	February 2019
CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody–Antigen Interface Liu, Yuhang; Pan, Junhua; Jenni, Simon Journal of Molecular Biology, Vol. 429, Issue 12 https://doi.org/10.1016/j.jmb.2017.05.011	journal	June 2017
Influenza hemagglutinin membrane anchor Benton, Donald J.; Nans, Andrea; Calder, Lesley J. Proceedings of the National Academy of Sciences, Vol. 115, Issue 40 https://doi.org/10.1073/pnas.1810927115	journal	September 2018
Mechanism of Enhanced Immature Dengue Virus Attachment to Endosomal Membrane Induced by prM Antibody Wirawan, Melissa; Fibriansah, Guntur; Marzinek, Jan K. Structure, Vol. 27, Issue 2 https://doi.org/10.1016/j.str.2018.10.009	journal	February 2019
Antibody affinity versus dengue morphology influences neutralization Fibriansah, Guntur; Lim, Elisa X. Y.; Marzinek, Jan K. PLOS Pathogens, Vol. 17, Issue 2 https://doi.org/10.1371/journal.ppat.1009331	journal	February 2021
Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers Fibriansah, Guntur; Ibarra, Kristie D.; Ng, Thiam-Seng Science, Vol. 349, Issue 6243 https://doi.org/10.1126/science.aaa8651	journal	July 2015
Structure basis of neutralization by a novel site II/IV antibody against respiratory syncytial virus fusion protein Xie, Qingqing; Wang, Zhao; Ni, Fengyun PLOS ONE, Vol. 14, Issue 2 https://doi.org/10.1371/journal.pone.0210749	journal	February 2019
Vaccination with prefusion-stabilized respiratory syncytial virus fusion protein induces genetically and antigenically diverse antibody responses Mukhamedova, Maryam; Wrapp, Daniel; Shen, Chen-Hsiang Immunity, Vol. 54, Issue 4 https://doi.org/10.1016/j.immuni.2021.03.004	journal	April 2021
How to Distinguish Conformational Selection and Induced Fit Based on Chemical Relaxation Rates Paul, Fabian; Weikl, Thomas R. PLOS Computational Biology, Vol. 12, Issue 9 https://doi.org/10.1371/journal.pcbi.1005067	journal	September 2016
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state Rossey, Iebe; Gilman, Morgan S. A.; Kabeche, Stephanie C. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14158	journal	February 2017
Innovations in structure-based antigen design and immune monitoring for next generation vaccines Ward, Andrew B.; Wilson, Ian A. Current Opinion in Immunology, Vol. 65 https://doi.org/10.1016/j.coi.2020.03.013	journal	August 2020
Mapping Polyclonal Antibody Responses in Non-human Primates Vaccinated with HIV Env Trimer Subunit Vaccines Nogal, Bartek; Bianchi, Matteo; Cottrell, Christopher A. Cell Reports, Vol. 30, Issue 11 https://doi.org/10.1016/j.celrep.2020.02.061	journal	March 2020
Polyclonal epitope mapping reveals temporal dynamics and diversity of human antibody responses to H5N1 vaccination Han, Julianna; Schmitz, Aaron J.; Richey, Sara T. Cell Reports, Vol. 34, Issue 4 https://doi.org/10.1016/j.celrep.2020.108682	journal	January 2021
High-resolution mapping of the neutralizing and binding specificities of polyclonal sera post-HIV Env trimer vaccination Dingens, Adam S.; Pratap, Payal; Malone, Keara eLife, Vol. 10 https://doi.org/10.7554/eLife.64281	journal	January 2021
Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization Bianchi, Matteo; Turner, Hannah L.; Nogal, Bartek Immunity, Vol. 49, Issue 2 https://doi.org/10.1016/j.immuni.2018.07.009	journal	August 2018
HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes Minor, Wladek; Cymborowski, Marcin; Otwinowski, Zbyszek Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8 https://doi.org/10.1107/S0907444906019949	journal	July 2006
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
cisTEM, user-friendly software for single-particle image processing Grant, Timothy; Rohou, Alexis; Grigorieff, Nikolaus eLife, Vol. 7 https://doi.org/10.7554/eLife.35383	journal	March 2018
CTFFIND4: Fast and accurate defocus estimation from electron micrographs Rohou, Alexis; Grigorieff, Nikolaus Journal of Structural Biology, Vol. 192, Issue 2 https://doi.org/10.1016/j.jsb.2015.08.008	journal	November 2015
Optimal Determination of Particle Orientation, Absolute Hand, and Contrast Loss in Single-particle Electron Cryomicroscopy Rosenthal, Peter B.; Henderson, Richard Journal of Molecular Biology, Vol. 333, Issue 4 https://doi.org/10.1016/j.jmb.2003.07.013	journal	October 2003
One number does not fit all: Mapping local variations in resolution in cryo-EM reconstructions Cardone, Giovanni; Heymann, J. Bernard; Steven, Alasdair C. Journal of Structural Biology, Vol. 184, Issue 2 https://doi.org/10.1016/j.jsb.2013.08.002	journal	November 2013
Real-space refinement in PHENIX for cryo-EM and crystallography Afonine, Pavel V.; Poon, Billy K.; Read, Randy J. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6 https://doi.org/10.1107/S2059798318006551	journal	May 2018
New tools for the analysis and validation of cryo-EM maps and atomic models Afonine, Pavel V.; Klaholz, Bruno P.; Moriarty, Nigel W. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 9 https://doi.org/10.1107/S2059798318009324	journal	September 2018
MolProbity: More and better reference data for improved all-atom structure validation: PROTEIN SCIENCE.ORG Williams, Christopher J.; Headd, Jeffrey J.; Moriarty, Nigel W. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3330	journal	November 2017
UCSF ChimeraX: Meeting modern challenges in visualization and analysis: UCSF ChimeraX Visualization System Goddard, Thomas D.; Huang, Conrad C.; Meng, Elaine C. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3235	journal	September 2017
GlyProt: in silico glycosylation of proteins Bohne-Lang, A.; von der Lieth, C. -W. Nucleic Acids Research, Vol. 33, Issue Web Server https://doi.org/10.1093/nar/gki385	journal	July 2005

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