Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation

Rauschendorfer, James; Yordanov, Yordan; Dobrev, Petre; Vankova, Radomira; Sykes, Robert; Külheim, Carsten; Busov, Victor

doi:10.1111/pbi.13309

Title: Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation

Journal Article · Wed Dec 04 00:00:00 EST 2019 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.13309· OSTI ID:1815595

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Michigan Technological Univ., Houghton, MI (United States). College of Forest Resources and Environmental Science
Eastern Illinois Univ., Charleston, IL (United States). Dept. of Biology
Czech Academy of Sciences, Prague (Czech Republic). Inst. of Experimental Botany
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Nuclear Materials Science

We investigated feasibility of the Full-length complementary DNA OvereXpression (FOX) system as a mutagenesis approach in poplar, using developing xylem tissue. The main goal was to assess the overall mutation rate and if the system will increase instances of mutants affected in traits linked to the xylem tissue. Indeed, we found a high mutation rate of 17.7%, whereas 80% of all mutants were significantly affected in cellulose, lignin and/or hemicellulose. Cell wall biosynthesis is a major process occurring during xylem development. Enrichment of mutants affected in cell wall composition suggests that the tissue source for the FOX library influenced the occurrence of mutants affected in a trait linked to this tissue. Additionally, we found that FLcDNAs from mutants affected in cell wall composition were homologous to genes known to be involved in cell wall biosynthesis and most recovered FLcDNAs corresponded to genes whose native expression was highest in xylem. We characterized in detail a mutant line with increased diameter. The phenotype was caused by a poplar homolog of LONELY GUY 1 (LOG1), which encodes an enzyme in cytokinin biosynthesis and significantly increased xylem proliferation. The causative role of LOG1 in the observed phenotype was further reaffirmed by elevated cytokinin concentration in the mutant and recapitulation overexpression experiment wherein multiple independent lines phenocopied the original FOX mutant. Our experiments show that the FOX approach can be efficiently used for gene discovery and molecular interrogation of traits specific to woody perennial growth and development.

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Research Organization:: Michigan Technological Univ., Houghton, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDA; Ministry of Education, Youth and Sports of the Czech Republic

Grant/Contract Number:: SC0008462; 2009-65504-05767; 1001498

OSTI ID:: 1815595

Journal Information:: Plant Biotechnology Journal, Vol. 18, Issue 6; ISSN 1467-7644

Publisher:: Society for Experimental Biology; Association of Applied BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation

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