Inhibitor Mimetic Mutations in the Pseudomonas aeruginosa PqsE Enzyme Reveal a Protein-Protein Interaction with the Quorum-Sensing Receptor RhlR That Is Vital for Virulence Factor Production

Taylor, I; Paczkowski, J.; Jeffrey, P.; Henke, B.; Smith, C.; Bassler, B.

doi:10.1021/acschembio.1c00049

Inhibitor Mimetic Mutations in the Pseudomonas aeruginosa PqsE Enzyme Reveal a Protein-Protein Interaction with the Quorum-Sensing Receptor RhlR That Is Vital for Virulence Factor Production

Journal Article · 01 January 2022 · ACS Chemical Biology

DOI:https://doi.org/10.1021/acschembio.1c00049· OSTI ID:1808999

Taylor, I; Paczkowski, J.; Jeffrey, P.; Henke, B.; Smith, C.; Bassler, B.

Research Organization:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Organization:: ~OTHER

DOE Contract Number:: SC0012704

OSTI ID:: 1808999

Report Number(s):: BNL-221894-2021-JACI

Journal Information:: ACS Chemical Biology, Vol. 16, Issue 4

Country of Publication:: United States

Language:: English

References (44)

The Epidemiology, Pathogenesis and Treatment of Pseudomonas aeruginosa Infections Driscoll, James A.; Brody, Steven L.; Kollef, Marin H. Drugs, Vol. 67, Issue 3 https://doi.org/10.2165/00003495-200767030-00003	journal	January 2007
Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients Smith, E. E.; Buckley, D. G.; Wu, Z. Proceedings of the National Academy of Sciences, Vol. 103, Issue 22 https://doi.org/10.1073/pnas.0602138103	journal	May 2006
Pseudomonas aeruginosa Biofilms: Host Response and Clinical Implications in Lung Infections Maurice, Nicholas M.; Bedi, Brahmchetna; Sadikot, Ruxana T. American Journal of Respiratory Cell and Molecular Biology, Vol. 58, Issue 4 https://doi.org/10.1165/rcmb.2017-0321TR	journal	April 2018
Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors Tan, M. -W.; Rahme, L. G.; Sternberg, J. A. Proceedings of the National Academy of Sciences, Vol. 96, Issue 5 https://doi.org/10.1073/pnas.96.5.2408	journal	March 1999
Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies Pang, Zheng; Raudonis, Renee; Glick, Bernard R. Biotechnology Advances, Vol. 37, Issue 1 https://doi.org/10.1016/j.biotechadv.2018.11.013	journal	January 2019
Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America Boucher, Helen W.; Talbot, George H.; Bradley, John S. Clinical Infectious Diseases, Vol. 48, Issue 1 https://doi.org/10.1086/595011	journal	January 2009
Hospital Mortality for Patients With Bacteremia Due to Staphylococcus aureus or Pseudomonas aeruginosa Osmon, Stephen; Ward, Suzanne; Fraser, Victoria J. Chest, Vol. 125, Issue 2 https://doi.org/10.1378/chest.125.2.607	journal	February 2004
Quorum Sensing in Bacteria Miller, Melissa B.; Bassler, Bonnie L. Annual Review of Microbiology, Vol. 55, Issue 1 https://doi.org/10.1146/annurev.micro.55.1.165	journal	October 2001
A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa. Pearson, J. P.; Passador, L.; Iglewski, B. H. Proceedings of the National Academy of Sciences, Vol. 92, Issue 5 https://doi.org/10.1073/pnas.92.5.1490	journal	February 1995
Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa Whiteley, M.; Lee, K. M.; Greenberg, E. P. Proceedings of the National Academy of Sciences, Vol. 96, Issue 24 https://doi.org/10.1073/pnas.96.24.13904	journal	November 1999
Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. Pesci, E. C.; Pearson, J. P.; Seed, P. C. Journal of bacteriology, Vol. 179, Issue 10 https://doi.org/10.1128/JB.179.10.3127-3132.1997	journal	January 1997
Quinolones: from antibiotics to autoinducers Heeb, Stephan; Fletcher, Matthew P.; Chhabra, Siri Ram FEMS Microbiology Reviews, Vol. 35, Issue 2 https://doi.org/10.1111/j.1574-6976.2010.00247.x	journal	March 2011
The role of pyocyanin in Pseudomonas aeruginosa infection Lau, Gee W.; Hassett, Daniel J.; Ran, Huimin Trends in Molecular Medicine, Vol. 10, Issue 12 https://doi.org/10.1016/j.molmed.2004.10.002	journal	December 2004
Exendin-4 restores airway mucus homeostasis through the GLP1R-PKA-PPARγ-FOXA2-phosphatase signaling Choi, Woosuk; Choe, Shawn; Lin, Jingjun Mucosal Immunology, Vol. 13, Issue 4 https://doi.org/10.1038/s41385-020-0262-1	journal	February 2020
PqsE of Pseudomonas aeruginosa Acts as Pathway-Specific Thioesterase in the Biosynthesis of Alkylquinolone Signaling Molecules Drees, Steffen Lorenz; Fetzner, Susanne Chemistry & Biology, Vol. 22, Issue 5 https://doi.org/10.1016/j.chembiol.2015.04.012	journal	May 2015
The PqsE and RhlR proteins are an autoinducer synthase–receptor pair that control virulence and biofilm development in Pseudomonas aeruginosa Mukherjee, Sampriti; Moustafa, Dina A.; Stergioula, Vasiliki Proceedings of the National Academy of Sciences, Vol. 115, Issue 40 https://doi.org/10.1073/pnas.1814023115	journal	September 2018
PqsE Functions Independently of PqsR- Pseudomonas Quinolone Signal and Enhances the rhl Quorum-Sensing System Farrow, John M.; Sund, Zoe M.; Ellison, Matthew L. Journal of Bacteriology, Vol. 190, Issue 21 https://doi.org/10.1128/JB.00753-08	journal	November 2008
PqsE Is Essential for RhlR-Dependent Quorum Sensing Regulation in Pseudomonas aeruginosa Groleau, Marie-Christine; de Oliveira Pereira, Thays; Dekimpe, Valérie mSystems, Vol. 5, Issue 3 https://doi.org/10.1128/mSystems.00194-20	journal	June 2020
The RhlR quorum-sensing receptor controls Pseudomonas aeruginosa pathogenesis and biofilm development independently of its canonical homoserine lactone autoinducer Mukherjee, Sampriti; Moustafa, Dina; Smith, Chari D. PLOS Pathogens, Vol. 13, Issue 7 https://doi.org/10.1371/journal.ppat.1006504	journal	July 2017
A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation O'Loughlin, C. T.; Miller, L. C.; Siryaporn, A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 44 https://doi.org/10.1073/pnas.1316981110	journal	October 2013
Structure–Function Analyses of the N -Butanoyl l -Homoserine Lactone Quorum-Sensing Signal Define Features Critical to Activity in RhlR Boursier, Michelle E.; Moore, Joseph D.; Heitman, Katherine M. ACS Chemical Biology, Vol. 13, Issue 9 https://doi.org/10.1021/acschembio.8b00577	journal	August 2018
Dissecting the Multiple Roles of PqsE in Pseudomonas aeruginosa Virulence by Discovery of Small Tool Compounds Zender, Michael; Witzgall, Florian; Drees, Steffen L. ACS Chemical Biology, Vol. 11, Issue 6 https://doi.org/10.1021/acschembio.6b00156	journal	April 2016
Identification of FDA-approved antivirulence drugs targeting the Pseudomonas aeruginosa quorum sensing effector protein PqsE Baldelli, Valerio; D’Angelo, Francesca; Pavoncello, Viola Virulence, Vol. 11, Issue 1 https://doi.org/10.1080/21505594.2020.1770508	journal	January 2020
Structure Elucidation and Preliminary Assessment of Hydrolase Activity of PqsE, the Pseudomonas Quinolone Signal (PQS) Response Protein Yu, Shen; Jensen, Vanessa; Seeliger, Janine Biochemistry, Vol. 48, Issue 43 https://doi.org/10.1021/bi900123j	journal	October 2009
Systematic Mutational Analysis of the Putative Hydrolase PqsE: Toward a Deeper Molecular Understanding of Virulence Acquisition in Pseudomonas aeruginosa Folch, Benjamin; Déziel, Eric; Doucet, Nicolas PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0073727	journal	September 2013
Discovery of PqsE Thioesterase Inhibitors for Pseudomonas aeruginosa Using DNA-Encoded Small Molecule Library Screening Valastyan, Julie S.; Tota, Michael R.; Taylor, Isabelle R. ACS Chemical Biology, Vol. 15, Issue 2 https://doi.org/10.1021/acschembio.9b00905	journal	December 2019
Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate Vaneechoutte, M.; Verschraegen, G.; Claeys, G. Journal of Clinical Microbiology, Vol. 26, Issue 6 https://doi.org/10.1128/JCM.26.6.1227-1228.1988	journal	June 1988
Fluorescence spectroscopic analysis of the structure and dynamics of Bacillus subtilis lipase A governing its activity profile under alkaline conditions Kübler, Daniel; Ingenbosch, Kim N.; Bergmann, Anna European Biophysics Journal, Vol. 44, Issue 8 https://doi.org/10.1007/s00249-015-1061-6	journal	July 2015
The Mechanisms of Catalysis by Metallo -Lactamases Page, Michael I.; Badarau, Adriana Bioinorganic Chemistry and Applications, Vol. 2008 https://doi.org/10.1155/2008/576297	journal	January 2008
Unravelling the Genome-Wide Contributions of Specific 2-Alkyl-4-Quinolones and PqsE to Quorum Sensing in Pseudomonas aeruginosa Rampioni, Giordano; Falcone, Marilena; Heeb, Stephan PLOS Pathogens, Vol. 12, Issue 11 https://doi.org/10.1371/journal.ppat.1006029	journal	November 2016
An autoinducer-independent RhlR quorum-sensing receptor enables analysis of RhlR regulation McCready, Amelia R.; Paczkowski, Jon E.; Cong, Jian-Ping PLOS Pathogens, Vol. 15, Issue 6 https://doi.org/10.1371/journal.ppat.1007820	journal	June 2019
Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms Lister, P. D.; Wolter, D. J.; Hanson, N. D. Clinical Microbiology Reviews, Vol. 22, Issue 4 https://doi.org/10.1128/CMR.00040-09	journal	October 2009
Features of MotA proton channel structure revealed by tryptophan-scanning mutagenesis. Sharp, L. L.; Zhou, J.; Blair, D. F. Proceedings of the National Academy of Sciences, Vol. 92, Issue 17 https://doi.org/10.1073/pnas.92.17.7946	journal	August 1995
Properties of the Mechanosensitive Channel MscS Pore Revealed by Tryptophan Scanning Mutagenesis Rasmussen, Tim; Rasmussen, Akiko; Singh, Shivani Biochemistry, Vol. 54, Issue 29 https://doi.org/10.1021/acs.biochem.5b00294	journal	July 2015
Visualizing transient protein-folding intermediates by tryptophan-scanning mutagenesis Vallée-Bélisle, Alexis; Michnick, Stephen W. Nature Structural & Molecular Biology, Vol. 19, Issue 7 https://doi.org/10.1038/nsmb.2322	journal	June 2012
Mutational mimics of allosteric effectors: a genome editing design to validate allosteric drug targets Tang, Qingling; Villar, Maria T.; Artigues, Antonio Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-45202-2	journal	June 2019
Resistance Mechanisms in Pseudomonas aeruginosa and Other Nonfermentative Gram‐Negative Bacteria Hancock, Robert E. W. Clinical Infectious Diseases, Vol. 27, Issue s1 https://doi.org/10.1086/514909	journal	August 1998
Pseudomonas aeruginosa: all roads lead to resistance Breidenstein, Elena B. M.; de la Fuente-Núñez, César; Hancock, Robert E. W. Trends in Microbiology, Vol. 19, Issue 8 https://doi.org/10.1016/j.tim.2011.04.005	journal	August 2011
Structural determinants driving homoserine lactone ligand selection in the Pseudomonas aeruginosa LasR quorum-sensing receptor McCready, Amelia R.; Paczkowski, Jon E.; Henke, Brad R. Proceedings of the National Academy of Sciences, Vol. 116, Issue 1 https://doi.org/10.1073/pnas.1817239116	journal	December 2018
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation Choi, Kyoung-Hee; Kumar, Ayush; Schweizer, Herbert P. Journal of Microbiological Methods, Vol. 64, Issue 3 https://doi.org/10.1016/j.mimet.2005.06.001	journal	March 2006
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Liebschner, Dorothee; Afonine, Pavel V.; Baker, Matthew L. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10 https://doi.org/10.1107/S2059798319011471	journal	October 2019

Similar Records

Inhibitor Mimetic Mutations in the Pseudomonas aeruginosa PqsE Enzyme Reveal a Protein–Protein Interaction with the Quorum-Sensing Receptor RhlR That Is Vital for Virulence Factor Production

Journal Article · 2021 · ACS Chemical Biology · OSTI ID:1816194

Taylor, Isabelle R.; Paczkowski, Jon E.; Jeffrey, Philip D.; +3 more

Molecular Basis for the Recognition of Structurally Distinct Autoinducer Mimics by the Pseudomonas aeruginosa LasR Quorum-Sensing Signaling Receptor

Journal Article · 2010 · Chem. Biol. · OSTI ID:1006059

Zou, Yaozhong; Nair, Satish K

The Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxododecanoyl) homoserine lactone enhances keratinocyte migration and induces Mmp13 gene expression in vitro

Journal Article · 2012 · Biochemical and Biophysical Research Communications · OSTI ID:22210289

Paes, Camila; Nakagami, Gojiro; Minematsu, Takeo; +4 more

Related Subjects

99 GENERAL AND MISCELLANEOUS

Inhibitor Mimetic Mutations in the Pseudomonas aeruginosa PqsE Enzyme Reveal a Protein-Protein Interaction with the Quorum-Sensing Receptor RhlR That Is Vital for Virulence Factor Production

Citation Formats

References (44)

Similar Records

Related Subjects