Conversion of High-Solids Hydrothermally Pretreated Bioenergy Sorghum to Lipids and Ethanol Using Yeast Cultures

Cheng, Ming-Hsun; Dien, Bruce Stuart; Jin, Yong-Su; Thompson, Stephanie; Shin, Jonghyeok; Watson Slininger, Patricia J.; Qureshi, Nasib; Singh, Vijay

doi:10.1021/acssuschemeng.1c01629

Title: Conversion of High-Solids Hydrothermally Pretreated Bioenergy Sorghum to Lipids and Ethanol Using Yeast Cultures

Journal Article · Mon Jun 14 00:00:00 EDT 2021 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.1c01629· OSTI ID:1807583

Cheng, Ming-Hsun ^[1]; Dien, Bruce Stuart ^[2];

^[1]; Thompson, Stephanie ^[2]; Shin, Jonghyeok ^[1]; Watson Slininger, Patricia J. ^[2]; Qureshi, Nasib ^[2];

^[1]

Univ. of Illinois at Urbana-Champaign, IL (United States)
US Dept. of Agriculture (USDA), Peoria, IL (United States). Agricultural Research Service

Glucose and xylose are the major sugars in cellulosic hydrolysates. The cellulosic sugars can be used for the production of biofuels and value-added bioproducts. In this study, lipid and ethanol were produced from bioenergy sorghum syrups using engineered yeasts. Here, bioenergy sorghum was hydrothermally pretreated at 50% solids loading in a continuous reactor system and mechanically refined sequentially using a burr mill to improve biomass accessibility for hydrolysis. Fed-batch enzymatic hydrolysis was conducted with 50% w/v solids loading to achieve 230 g/L sugar concentration. Different strains of Rhodosporidium toruloides were used to ferment sugars into lipids, and the highest lipid yield of 9.2 g/L was observed. The lipid yield was improved to 19.0 g/l by implementing a two-stage culture where once the sugars were exhausted in the first stage, the yeast was introduced into fresh hydrolysate without adding nitrogen. For ethanol production, the engineered Saccharomyces cerevisiae SR8ΔADH6 was utilized to coferment glucose and xylose. Additionally, the effects of nutrient media (YP, YNB/urea, and urea), cellulosic sugar concentration, and sulfite addition were investigated to optimize the ethanol yield from sorghum syrups. The optimal ethanol yield at 73.3% was obtained from the YNB/urea culture consisting of 34 g glucose/L and 17 g xylose/L.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 1807583

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 9, Issue 25; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

References (48)

Microbial lipid-based lignocellulosic biorefinery: feasibility and challenges Jin, Mingjie; Slininger, Patricia J.; Dien, Bruce S. Trends in Biotechnology, Vol. 33, Issue 1 https://doi.org/10.1016/j.tibtech.2014.11.005	journal	January 2015
Oleaginous yeasts for sustainable lipid production—from biodiesel to surf boards, a wide range of “green” applications Vasconcelos, Bruno; Teixeira, José Carlos; Dragone, Giuliano Applied Microbiology and Biotechnology, Vol. 103, Issue 9 https://doi.org/10.1007/s00253-019-09742-x	journal	March 2019
Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid Yu, Xiaochen; Zheng, Yubin; Dorgan, Kathleen M. Bioresource Technology, Vol. 102, Issue 10 https://doi.org/10.1016/j.biortech.2011.02.081	journal	May 2011
Sugar production from bioenergy sorghum by using pilot scale continuous hydrothermal pretreatment combined with disk refining Cheng, Ming-Hsun; Dien, Bruce S.; Lee, D. K. Bioresource Technology, Vol. 289 https://doi.org/10.1016/j.biortech.2019.121663	journal	October 2019
Lipid metabolism of the oleaginous yeast Lipomyces starkeyi Takaku, Hiroaki; Matsuzawa, Tomohiko; Yaoi, Katsuro Applied Microbiology and Biotechnology, Vol. 104, Issue 14 https://doi.org/10.1007/s00253-020-10695-9	journal	May 2020
Challenges in biodiesel industry with regards to feedstock, environmental, social and sustainability issues: A critical review Anuar, Mohd Razealy; Abdullah, Ahmad Zuhairi Renewable and Sustainable Energy Reviews, Vol. 58 https://doi.org/10.1016/j.rser.2015.12.296	journal	May 2016
Lipid production via simultaneous conversion of glucose and xylose by a novel yeast, Cystobasidium iriomotense Tanimura, Ayumi; Sugita, Takashi; Endoh, Rikiya PLOS ONE, Vol. 13, Issue 9 https://doi.org/10.1371/journal.pone.0202164	journal	September 2018
Lipid production for second generation biodiesel by the oleaginous yeast Rhodotorula graminis Galafassi, Silvia; Cucchetti, Daniela; Pizza, Francesca Bioresource Technology, Vol. 111 https://doi.org/10.1016/j.biortech.2012.02.004	journal	May 2012
Glucose-mediated regulation of glycerol uptake in Rhodosporidium toruloides : Insights through transcriptomic analysis on dual substrate fermentation Bommareddy, Rajesh Reddy; Sabra, Wael; Zeng, An-Ping Engineering in Life Sciences, Vol. 17, Issue 3 https://doi.org/10.1002/elsc.201600010	journal	June 2016
Improvement of sugar yields from corn stover using sequential hot water pretreatment and disk milling Kim, Sun Min; Dien, Bruce S.; Tumbleson, M. E. Bioresource Technology, Vol. 216 https://doi.org/10.1016/j.biortech.2016.06.003	journal	September 2016
Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts Chmielarz, Mikolaj; Blomqvist, Johanna; Sampels, Sabine Biotechnology for Biofuels, Vol. 14, Issue 1 https://doi.org/10.1186/s13068-021-01916-y	journal	March 2021
Identification of superior lipid producing Lipomyces and Myxozyma yeasts S. Dien, Bruce; J. Slininger, Patricia; P. Kurtzman, Cletus AIMS Environmental Science, Vol. 3, Issue 1 https://doi.org/10.3934/environsci.2016.1.1	journal	January 2016
Efficient bioconversion of enzymatic corncob hydrolysate into biomass and lipids by oleaginous yeast Rhodosporidium paludigenum KM281510 Chaiyaso, Thanongsak; Manowattana, Atchara; Techapun, Charin Preparative Biochemistry and Biotechnology, Vol. 49, Issue 6 https://doi.org/10.1080/10826068.2019.1591985	journal	March 2019
Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion Turner, Timothy L.; Zhang, Guo-Chang; Kim, Soo Rin Applied Microbiology and Biotechnology, Vol. 99, Issue 19 https://doi.org/10.1007/s00253-015-6701-3	journal	June 2015
Cost and Life-Cycle Greenhouse Gas Implications of Integrating Biogas Upgrading and Carbon Capture Technologies in Cellulosic Biorefineries Yang, Minliang; Baral, Nawa Raj; Anastasopoulou, Aikaterini Environmental Science & Technology, Vol. 54, Issue 20 https://doi.org/10.1021/acs.est.0c02816	journal	October 2020
Enzymatic hydrolysis of biomass at high-solids loadings – A review Modenbach, Alicia A.; Nokes, Sue E. Biomass and Bioenergy, Vol. 56 https://doi.org/10.1016/j.biombioe.2013.05.031	journal	September 2013
A survey of yeast from the Yarrowia clade for lipid production in dilute acid pretreated lignocellulosic biomass hydrolysate Quarterman, Josh; Slininger, Patricia J.; Kurtzman, Cletus P. Applied Microbiology and Biotechnology, Vol. 101, Issue 8 https://doi.org/10.1007/s00253-016-8062-y	journal	December 2016
Lipid production in batch and fed-batch cultures of Rhodosporidium toruloidesfrom 5 and 6 carbon carbohydrates Wiebe, Marilyn G.; Koivuranta, Kari; Penttilä, Merja BMC Biotechnology, Vol. 12, Issue 1 https://doi.org/10.1186/1472-6750-12-26	journal	May 2012
Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion Zhang, Guo-Chang; Liu, Jing-Jing; Kong, In Iok Current Opinion in Chemical Biology, Vol. 29 https://doi.org/10.1016/j.cbpa.2015.09.008	journal	December 2015
Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species Sitepu, Irnayuli R.; Sestric, Ryan; Ignatia, Laura Bioresource Technology, Vol. 144 https://doi.org/10.1016/j.biortech.2013.06.047	journal	September 2013
Comparative lipid production by oleaginous yeasts in hydrolyzates of lignocellulosic biomass and process strategy for high titers: High Titer Lipid Production by Yeasts in Lignocellulosic Hydrolyzates Slininger, Patricia J.; Dien, Bruce S.; Kurtzman, Cletus P. Biotechnology and Bioengineering, Vol. 113, Issue 8 https://doi.org/10.1002/bit.25928	journal	February 2016
Yeast as a cell factory: current state and perspectives Kavšček, Martin; Stražar, Martin; Curk, Tomaž Microbial Cell Factories, Vol. 14, Issue 1 https://doi.org/10.1186/s12934-015-0281-x	journal	June 2015
The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: a reappraisal and unsolved problems Ratledge, Colin Biotechnology Letters, Vol. 36, Issue 8 https://doi.org/10.1007/s10529-014-1532-3	journal	April 2014
Microbial lipid production from renewable and waste materials for second-generation biodiesel feedstock Muniraj, Iniya Kumar; Uthandi, Siva Kumar; Hu, Zhenhu Environmental Technology Reviews, Vol. 4, Issue 1 https://doi.org/10.1080/21622515.2015.1018340	journal	January 2015
High solids loading biorefinery for the production of cellulosic sugars from bioenergy sorghum Cheng, Ming-Hsun; Kadhum, Haider Jawad; Murthy, Ganti S. Bioresource Technology, Vol. 318 https://doi.org/10.1016/j.biortech.2020.124051	journal	December 2020
Microbial lipids as potential source to food supplements Béligon, Vanessa; Christophe, Gwendoline; Fontanille, Pierre Current Opinion in Food Science, Vol. 7 https://doi.org/10.1016/j.cofs.2015.10.002	journal	February 2016
The isocitrate dehydrogenase gene of oleaginous yeast Lipomyces starkeyi is linked to lipid accumulation Tang, Wei; Zhang, Sufang; Wang, Qian Canadian Journal of Microbiology, Vol. 55, Issue 9 https://doi.org/10.1139/W09-063	journal	September 2009
Dual utilization of NADPH and NADH cofactors enhances xylitol production in engineered Saccharomyces cerevisiae Jo, Jung-Hyun; Oh, Sun-Young; Lee, Hyeun-Soo Biotechnology Journal, Vol. 10, Issue 12 https://doi.org/10.1002/biot.201500068	journal	November 2015
Oily yeasts as oleaginous cell factories Ageitos, Jose Manuel; Vallejo, Juan Andres; Veiga-Crespo, Patricia Applied Microbiology and Biotechnology, Vol. 90, Issue 4 https://doi.org/10.1007/s00253-011-3200-z	journal	April 2011
“Designer” Biodiesel: Optimizing Fatty Ester Composition to Improve Fuel Properties ^† Knothe, Gerhard Energy & Fuels, Vol. 22, Issue 2 https://doi.org/10.1021/ef700639e	journal	March 2008
Agro-industrial lignocellulosic biomass a key to unlock the future bio-energy: A brief review Anwar, Zahid; Gulfraz, Muhammad; Irshad, Muhammad Journal of Radiation Research and Applied Sciences, Vol. 7, Issue 2 https://doi.org/10.1016/j.jrras.2014.02.003	journal	April 2014
A comparative account of glucose yields and bioethanol production from separate and simultaneous saccharification and fermentation processes at high solids loading with variable PEG concentration Kadhum, Haider Jawad; Mahapatra, Durga Madhab; Murthy, Ganti S. Bioresource Technology, Vol. 283 https://doi.org/10.1016/j.biortech.2019.03.060	journal	July 2019
Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production Gong, Zhiwei; Wang, Qian; Shen, Hongwei Bioresource Technology, Vol. 117 https://doi.org/10.1016/j.biortech.2012.04.063	journal	August 2012
Enhanced production of 2,3-butanediol from xylose by combinatorial engineering of xylose metabolic pathway and cofactor regeneration in pyruvate decarboxylase-deficient Saccharomyces cerevisiae Kim, Soo-Jung; Sim, Hee-Jin; Kim, Jin-Woo Bioresource Technology, Vol. 245 https://doi.org/10.1016/j.biortech.2017.06.034	journal	December 2017
The Biochemistry and Molecular Biology of Lipid Accumulation in Oleaginous Microorganisms Ratledge, Colin; Wynn, James P. Advances in Applied Microbiology https://doi.org/10.1016/S0065-2164(02)51000-5	book	January 2002
Production of d-arabitol from d-xylose by the oleaginous yeast Rhodosporidium toruloides IFO0880 Jagtap, Sujit Sadashiv; Rao, Christopher V. Applied Microbiology and Biotechnology, Vol. 102, Issue 1 https://doi.org/10.1007/s00253-017-8581-1	journal	November 2017
Enzymatic hydrolysates of corn stover pretreated by a N-methylpyrrolidone–ionic liquid solution for microbial lipid production Xie, Haibo; Shen, Hongwei; Gong, Zhiwei Green Chemistry, Vol. 14, Issue 4 https://doi.org/10.1039/c2gc00033d	journal	January 2012
Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST) Lau, M. W.; Dale, B. E. Proceedings of the National Academy of Sciences, Vol. 106, Issue 5, p. 1368-1373 https://doi.org/10.1073/pnas.0812364106	journal	January 2009
The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica Wasylenko, Thomas M.; Ahn, Woo Suk; Stephanopoulos, Gregory Metabolic Engineering, Vol. 30 https://doi.org/10.1016/j.ymben.2015.02.007	journal	July 2015
Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production Papanikolaou, Seraphim; Aggelis, George European Journal of Lipid Science and Technology, Vol. 113, Issue 8 https://doi.org/10.1002/ejlt.201100014	journal	June 2011
Biogenesis and functions of lipid droplets in plants: Thematic Review Series: Lipid Droplet Synthesis and Metabolism: from Yeast to Man Chapman, Kent D.; Dyer, John M.; Mullen, Robert T. Journal of Lipid Research, Vol. 53, Issue 2 https://doi.org/10.1194/jlr.R021436	journal	November 2011
Lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by Pseudozyma hubeiensis: a comparative screening study Tanimura, Ayumi; Takashima, Masako; Sugita, Takashi AMB Express, Vol. 6, Issue 1 https://doi.org/10.1186/s13568-016-0236-6	journal	August 2016
Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways Cunha, Joana T.; Soares, Pedro O.; Romaní, Aloia Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1360-8	journal	January 2019
The use of high-solids loadings in biomass pretreatment-a review Modenbach, Alicia A.; Nokes, Sue E. Biotechnology and Bioengineering, Vol. 109, Issue 6 https://doi.org/10.1002/bit.24464	journal	February 2012
Production of xylose enriched hydrolysate from bioenergy sorghum and its conversion to β-carotene using an engineered Saccharomyces cerevisiae Cheng, Ming-Hsun; Sun, Liang; Jin, Yong-Su Bioresource Technology, Vol. 308 https://doi.org/10.1016/j.biortech.2020.123275	journal	July 2020
Vitamin A Production by Engineered Saccharomyces cerevisiae from Xylose via Two-Phase in Situ Extraction Sun, Liang; Kwak, Suryang; Jin, Yong-Su ACS Synthetic Biology, Vol. 8, Issue 9 https://doi.org/10.1021/acssynbio.9b00217	journal	August 2019
Effect of sodium sulfite on acid pretreatment of wheat straw with respect to its final conversion to ethanol Jaisamut, Kitipong; Paulová, Leona; Patáková, Petra Biomass and Bioenergy, Vol. 95 https://doi.org/10.1016/j.biombioe.2016.08.022	journal	December 2016
Technoeconomic and life-cycle analysis of single-step catalytic conversion of wet ethanol into fungible fuel blendstocks Hannon, John R.; Lynd, Lee R.; Andrade, Onofre Proceedings of the National Academy of Sciences, Vol. 117, Issue 23 https://doi.org/10.1073/pnas.1821684116	journal	November 2019

Similar Records

A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach

Journal Article · Wed Dec 21 00:00:00 EST 2022 · Fermentation · OSTI ID:1807583

Deshavath, Narendra Naik; Dien, Bruce S.; Slininger, Patricia J.; +2 more

Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production

Journal Article · Wed Nov 29 00:00:00 EST 2023 · Biotechnology for Biofuels and Bioproducts · OSTI ID:1807583

Woodruff, William; Deshavath, Narendra Naik; Susanto, Vionna; +2 more

Production of xylose enriched hydrolysate from bioenergy sorghum and its conversion to β-carotene using an engineered Saccharomyces cerevisiae

Journal Article · Wed Jul 01 00:00:00 EDT 2020 · Bioresource Technology · OSTI ID:1807583

Cheng, Ming-Hsun; Sun, Liang; Jin, Yong-Su; +2 more

Related Subjects

09 BIOMASS FUELS
Bioenergy sorghum
Rhodosporidium toruloides
Saccharomyces cerevisiae
Lipid production
Ethanol production

Title: Conversion of High-Solids Hydrothermally Pretreated Bioenergy Sorghum to Lipids and Ethanol Using Yeast Cultures

Citation Formats

References (48)

Similar Records

Related Subjects