Oscillatory phase separation during expulsion of daughter GUVs

Multimedia · 01 October 2014

DOI:https://doi.org/10.7554/eLife.03695.018· OSTI ID:1806205

Oglecka, Kamila ^[1]; Rangamani, Padmini; Liedberg, Bo; Kraut, Rachel S; Parikh, Atul N

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1. DOEOSTI

A video assembled from time-lapse fluorescence images of phase-separating GUVs containing internal ‘organelle’ vesicles. GUVs consisting of POPC:SM:Ch (2:2:1) labeled with 0.5 mol% Sphingomyelin-Atto647N (SM-647N) (pseudo-colored green), encapsulate 1 M sucrose, and diluted in MilliQ water at 25°C. The video reveals shifting patterns of osmotic pressure and tension during expulsion of the internal vesicles after an osmotic differential had been established. Key steps include (A) a homogeneous, flaccid mother vesicle encapsulating tense daughter vesicles, at a time point prior to vesicle expulsion; (B) just after expulsion, the daughter GUV remains tense exhibiting oscillatory phase separation, while the mother GUV is left deflated and homogenous due to the sudden loss of volume; (C) The mother GUV subsequently becomes inflated by influx of water; and (D) the mother GUV begins to exhibit oscillatory phase separation.

• Research Organization: ELIFE-C (eLife Sciences Publications, Ltd.)
• OSTI ID: 1806205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL BIOLOGY
COMPARTMENTALIZATION
GIANT PHOSPHOLIPID VESICLE
GUV
LIPID RAFT
PHASE SEPARATION
PRIMITIVE OSMOREGULATION
RESEARCH ARTICLE