GUVs prepared by gentle hydration reproduce the oscillatory domain dynamics

Multimedia · 01 October 2014

DOI:https://doi.org/10.7554/eLife.03695.011· OSTI ID:1806200

Oglecka, Kamila ^[1]; Rangamani, Padmini; Liedberg, Bo; Kraut, Rachel S; Parikh, Atul N

---

1. DOEOSTI

GUVs prepared by ‘gentle hydration’ consisting of POPC:SM:Ch (1:1:1) labeled with 0.5 mol% Rho-DPPE (pseudo-colored blue), encapsulating 200 mM sucrose. Hypotonic conditions are established by dilution in deionized water at 25°C. Vesicles exhibit osmotic swelling and oscillatory domain dynamics comparable to that seen for electroformed GUVs under the same conditions.

• Research Organization: ELIFE-C (eLife Sciences Publications, Ltd.)
• OSTI ID: 1806200
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL BIOLOGY
COMPARTMENTALIZATION
GIANT PHOSPHOLIPID VESICLE
GUV
LIPID RAFT
PHASE SEPARATION
PRIMITIVE OSMOREGULATION
RESEARCH ARTICLE