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Title: A comparative look at structural variation among RC–LH1 ‘Core’ complexes present in anoxygenic phototrophic bacteria

Abstract

Abstract All purple photosynthetic bacteria contain RC–LH1 ‘Core’ complexes. The structure of this complex fromRhodobacter sphaeroides,Rhodopseudomonas palustrisandThermochromatium tepidumhas been solved using X-ray crystallography. Recently, the application of single particle cryo-EM has revolutionised structural biology and the structure of the RC–LH1 ‘Core’ complex fromBlastochloris viridishas been solved using this technique, as well as the complex from the non-purple Chloroflexi species,Roseiflexus castenholzii. It is apparent that these structures are variations on a theme, although with a greater degree of structural diversity within them than previously thought. Furthermore, it has recently been discovered that the only phototrophic representative from the phylum Gemmatimonadetes,Gemmatimonas phototrophica, also contains a RC–LH1 ‘Core’ complex. At present only a low-resolution EM-projection map exists but this shows that theGemmatimonas phototrophicacomplex contains a double LH1 ring. This short review compares these different structures and looks at the functional significance of these variations from two main standpoints: energy transfer and quinone exchange.

Authors:
ORCiD logo; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1801407
DOE Contract Number:  
SC0001035
Resource Type:
Journal Article
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 145; Journal Issue: 2; Journal ID: ISSN 0166-8595
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
Plant Sciences

Citation Formats

Gardiner, Alastair T., Nguyen-Phan, Tu C., and Cogdell, Richard J.. A comparative look at structural variation among RC–LH1 ‘Core’ complexes present in anoxygenic phototrophic bacteria. United States: N. p., 2020. Web. doi:10.1007/s11120-020-00758-3.
Gardiner, Alastair T., Nguyen-Phan, Tu C., & Cogdell, Richard J.. A comparative look at structural variation among RC–LH1 ‘Core’ complexes present in anoxygenic phototrophic bacteria. United States. https://doi.org/10.1007/s11120-020-00758-3
Gardiner, Alastair T., Nguyen-Phan, Tu C., and Cogdell, Richard J.. Tue . "A comparative look at structural variation among RC–LH1 ‘Core’ complexes present in anoxygenic phototrophic bacteria". United States. https://doi.org/10.1007/s11120-020-00758-3.
@article{osti_1801407,
title = {A comparative look at structural variation among RC–LH1 ‘Core’ complexes present in anoxygenic phototrophic bacteria},
author = {Gardiner, Alastair T. and Nguyen-Phan, Tu C. and Cogdell, Richard J.},
abstractNote = {Abstract All purple photosynthetic bacteria contain RC–LH1 ‘Core’ complexes. The structure of this complex fromRhodobacter sphaeroides,Rhodopseudomonas palustrisandThermochromatium tepidumhas been solved using X-ray crystallography. Recently, the application of single particle cryo-EM has revolutionised structural biology and the structure of the RC–LH1 ‘Core’ complex fromBlastochloris viridishas been solved using this technique, as well as the complex from the non-purple Chloroflexi species,Roseiflexus castenholzii. It is apparent that these structures are variations on a theme, although with a greater degree of structural diversity within them than previously thought. Furthermore, it has recently been discovered that the only phototrophic representative from the phylum Gemmatimonadetes,Gemmatimonas phototrophica, also contains a RC–LH1 ‘Core’ complex. At present only a low-resolution EM-projection map exists but this shows that theGemmatimonas phototrophicacomplex contains a double LH1 ring. This short review compares these different structures and looks at the functional significance of these variations from two main standpoints: energy transfer and quinone exchange.},
doi = {10.1007/s11120-020-00758-3},
url = {https://www.osti.gov/biblio/1801407}, journal = {Photosynthesis Research},
issn = {0166-8595},
number = 2,
volume = 145,
place = {United States},
year = {2020},
month = {5}
}

Works referenced in this record:

Control of Synthesis of Reaction Center Bacteriochlorophyll in Photosynthetic Bacteria
journal, February 1972


The Light-Harvesting Polypeptides of Rhodopseudomonas viridis. The Complete Amino-Acid Sequences of B1015-α, B1015-β and B1015-γ
journal, January 1985


Spectroscopic Analysis of Bacteriochlorophylls in Vitro and in vivo
journal, August 1966


The purple heart of photosynthesis
journal, March 2014


Kinetics and energetics of electron transfer in reaction centers of the photosynthetic bacterium Roseiflexus castenholzii
journal, March 2011


Light-Harvesting Antenna System from the Phototrophic Bacterium Roseiflexus castenholzii
journal, September 2010


An Ab Initio Description of the Excitonic Properties of LH2 and Their Temperature Dependence
journal, October 2016


Unique double concentric ring organization of light harvesting complexes in Gemmatimonas phototrophica
journal, December 2017


A new bacterial chlorophyll
journal, February 1963


The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila
journal, November 1993


Architecture and mechanism of the light-harvesting apparatus of purple bacteria
journal, May 1998


Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex
journal, February 2018


The peripheral light-harvesting complexes from purple sulfur bacteria have different ‘ring’ sizes
journal, October 2008


Biochemical and Spectroscopic Characterizations of a Hybrid Light-Harvesting Reaction Center Core Complex
journal, July 2018


Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales
journal, July 2017


NMR as a tool to investigate the structure, dynamics and function of membrane proteins
journal, June 2016


Suppressor mutants of the photosynthetically incompetent pufX deletion mutant Rhodobacter capsulatus ΔRC6(pTL2)
journal, December 1992


Structural basis of energy transfer in Porphyridium purpureum phycobilisome
journal, February 2020


Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria
journal, April 1995


Three-dimensional structure of a photosynthetic membrane
journal, November 1982


Structure of the LH1–RC complex from Thermochromatium tepidum at 3.0 Å
journal, March 2014


Profile of Joachim Frank, Richard Henderson, and Jacques Dubochet, 2017 Nobel Laureates in Chemistry
journal, December 2017


The role of charge-transfer states in the spectral tuning of antenna complexes of purple bacteria
journal, March 2018


The PufX quinone channel enables the light-harvesting 1 antenna to bind more carotenoids for light collection and photoprotection
journal, February 2017


Spectral Irradiance and Distribution of Pigments in a Highly Layered Marine Microbial Mat
journal, August 1990


The C-terminus of PufX plays a key role in dimerisation and assembly of the reaction center light-harvesting 1 complex from Rhodobacter sphaeroides
journal, September 2017


Cryo-EM structure of the Blastochloris viridis LH1–RC complex at 2.9 Å
journal, April 2018


Refinement of the x-ray structure of the RC LH1 core complex from Rhodopseudomonas palustris by single-molecule spectroscopy
journal, December 2007


Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM
journal, February 2003


Structure of the dimeric RC–LH1–PufX complex from Rhodobaca bogoriensis investigated by electron microscopy
journal, December 2012

  • Semchonok, Dmitry A.; Chauvin, Jean-Paul; Frese, Raoul N.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 367, Issue 1608
  • https://doi.org/10.1098/rstb.2012.0063

Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes
journal, May 2017


The structure of the photoreceptor unit of Rhodopseudomonas viridis
journal, April 1984


Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna
journal, March 2019


Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting
journal, November 2017


The solution structure of the PufX polypeptide from Rhodobacter sphaeroides
journal, December 2006


Cryo-EM structure of the RC-LH core complex from an early branching photosynthetic prokaryote
journal, April 2018


Novel features of LH1‐RC from Thermochromatium tepidum revealed from its atomic resolution structure
journal, October 2018


Functional type 2 photosynthetic reaction centers found in the rare bacterial phylum Gemmatimonadetes
journal, May 2014


Structure of phycobilisome from the red alga Griffithsia pacifica
journal, October 2017