Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization

Whitaker, William B.; Sandoval, Nicholas R.; Bennett, Robert K.; Fast, Alan G.; Papoutsakis, Eleftherios T.

doi:10.1016/j.copbio.2015.01.007

Title: Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization

Journal Article · Thu Mar 19 00:00:00 EDT 2015 · Current Opinion in Biotechnology

DOI:https://doi.org/10.1016/j.copbio.2015.01.007· OSTI ID:1749883

Whitaker, William B. ^[1]; Sandoval, Nicholas R. ^[1]; Bennett, Robert K. ^[1]; Fast, Alan G. ^[1]; Papoutsakis, Eleftherios T. ^[1]

Univ. of Delaware, Newark, DE (United States)

Synthetic methylotrophy is the development of non-native methylotrophs that can utilize methane and methanol as sole carbon and energy sources or as co-substrates with carbohydrates to produce metabolites as biofuels and chemicals. Here, the availability of methane (from natural gas) and its oxidation product, methanol, has been increasing, while prices have been decreasing, thus rendering them as attractive fermentation substrates. As they are more reduced than most carbohydrates, methane and methanol, as co-substrates, can enhance the yields of biologically produced metabolites. Here we discuss synthetic biology and metabolic engineering strategies based on the native biology of aerobic methylotrophs for developing synthetic strains grown on methanol, with Escherichia coli as the prototype.

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Research Organization:: Univ. of Delaware, Newark, DE (United States)

Sponsoring Organization:: USDOE Advanced Research Projects Agency - Energy (ARPA-E)

Grant/Contract Number:: AR0000432

OSTI ID:: 1749883

Journal Information:: Current Opinion in Biotechnology, Vol. 33; ISSN 0958-1669

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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