skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production

Abstract

Methanol is an attractive and broadly available substrate for large-scale bioproduction of fuels and chemicals. It contains more energy and electrons per carbon than carbohydrates and can be cheaply produced from natural gas. Synthetic methylotrophy refers to the development of non-native methylotrophs such as Escherichia coli and Corynebacterium glutamicum to utilize methanol as a carbon source. Furthermore, we discuss recent advances in engineering these industrial hosts to assimilate methanol for growth and chemicals production through the introduction of the ribulose monophosphate (RuMP) cycle. In addition, we present novel strategies based on flux coupling and adaptive laboratory evolution to engineer new strains that can grow exclusively on methanol.

Authors:
ORCiD logo [1]
  1. Univ. of Delaware, Newark, DE (United States)
Publication Date:
Research Org.:
Univ. of Delaware, Newark, DE (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
1747974
Grant/Contract Number:  
AR0000432
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Current Opinion in Biotechnology
Additional Journal Information:
Journal Volume: 59; Journal ID: ISSN 0958-1669
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Antoniewicz, Maciek R. Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production. United States: N. p., 2019. Web. doi:10.1016/j.copbio.2019.07.001.
Antoniewicz, Maciek R. Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production. United States. https://doi.org/10.1016/j.copbio.2019.07.001
Antoniewicz, Maciek R. Mon . "Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production". United States. https://doi.org/10.1016/j.copbio.2019.07.001. https://www.osti.gov/servlets/purl/1747974.
@article{osti_1747974,
title = {Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production},
author = {Antoniewicz, Maciek R.},
abstractNote = {Methanol is an attractive and broadly available substrate for large-scale bioproduction of fuels and chemicals. It contains more energy and electrons per carbon than carbohydrates and can be cheaply produced from natural gas. Synthetic methylotrophy refers to the development of non-native methylotrophs such as Escherichia coli and Corynebacterium glutamicum to utilize methanol as a carbon source. Furthermore, we discuss recent advances in engineering these industrial hosts to assimilate methanol for growth and chemicals production through the introduction of the ribulose monophosphate (RuMP) cycle. In addition, we present novel strategies based on flux coupling and adaptive laboratory evolution to engineer new strains that can grow exclusively on methanol.},
doi = {10.1016/j.copbio.2019.07.001},
url = {https://www.osti.gov/biblio/1747974}, journal = {Current Opinion in Biotechnology},
issn = {0958-1669},
number = ,
volume = 59,
place = {United States},
year = {2019},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share: