Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Structural and mechanistic insights into Hsp104 function revealed by synchrotron X-ray footprinting

Journal Article · · Journal of Biological Chemistry

Research Organization:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Organization:
~OTHER
DOE Contract Number:
SC0012704
OSTI ID:
1689995
Report Number(s):
BNL-220323-2020-JACI
Journal Information:
Journal of Biological Chemistry, Vol. 295, Issue 6
Country of Publication:
United States
Language:
English

References (89)

Hsp104: A Weapon to Combat Diverse Neurodegenerative Disorders journal December 2007
Protein-Remodeling Factors As Potential Therapeutics for Neurodegenerative Disease journal February 2017
Engineering and Evolution of Molecular Chaperones and Protein Disaggregases with Enhanced Activity journal March 2016
Engineering therapeutic protein disaggregases journal May 2016
Designer protein disaggregases to counter neurodegenerative disease journal June 2017
AAA+ Protein-Based Technologies to Counter Neurodegenerative Disease journal April 2019
Therapeutic Dissolution of Aberrant Phases by Nuclear-Import Receptors journal April 2019
Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains journal April 2018
Spiraling in Control: Structures and Mechanisms of the Hsp104 Disaggregase journal February 2019
Mechanistic and Structural Insights into the Prion-Disaggregase Activity of Hsp104 journal May 2016
Reversible, Specific, Active Aggregates of Endogenous Proteins Assemble upon Heat Stress journal September 2015
Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules journal August 2015
Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery journal June 2018
Blessings in disguise: biological benefits of prion-like mechanisms journal June 2013
The elusive middle domain of Hsp104 and ClpB: Location and function journal January 2012
Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104 journal June 2017
Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation journal August 2016
Structure of Calcarisporiella thermophila Hsp104 Disaggregase that Antagonizes Diverse Proteotoxic Misfolding Events journal March 2019
The Hsp104 N-Terminal Domain Enables Disaggregase Plasticity and Potentiation journal March 2015
Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients journal November 2012
Protein disaggregation mediated by heat-shock protein Hspl04 journal December 1994
Hsp104, Hsp70, and Hsp40 journal July 1998
Hsp104 Catalyzes Formation and Elimination of Self-Replicating Sup35 Prion Conformers journal June 2004
Destruction or Potentiation of Different Prions Catalyzed by Similar Hsp104 Remodeling Activities journal August 2006
Asymmetric deceleration of ClpB or Hsp104 ATPase activity unleashes protein-remodeling activity journal January 2007
Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions journal October 2008
The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System journal October 2011
Hsp104 and Potentiated Variants Can Operate as Distinct Nonprocessive Translocases journal May 2019
Avidity for Polypeptide Binding by Nucleotide-Bound Hsp104 Structures journal April 2017
Hydrogen exchange reveals Hsp104 architecture, structural dynamics, and energetics in physiological solution journal March 2019
Radiolytic Protein Footprinting with mass Spectrometry to Probe the Structure of Macromolecular Complexes journal June 2006
Hydroxyl radical probe of protein surfaces using synchrotron X-ray radiolysis and mass spectrometry journal January 2002
Hydroxyl radical probe of the surface of lysozyme by synchrotron radiolysis and mass spectrometry journal January 2001
Conserved Distal Loop Residues in the Hsp104 and ClpB Middle Domain Contact Nucleotide-binding Domain 2 and Enable Hsp70-dependent Protein Disaggregation journal January 2014
RNA Folding at Millisecond Intervals by Synchrotron Hydroxyl Radical Footprinting journal March 1998
Development of Synchrotron Footprinting at NSLS and NSLS-II journal February 2019
The Structure of ClpB journal October 2003
Crystal structures of Hsp104 N-terminal domains from Saccharomyces cerevisiae and Candida albicans suggest the mechanism for the function of Hsp104 in dissolving prions journal March 2017
Crystallographic investigation of peptide binding sites in the N-domain of the ClpA chaperone journal April 2004
Crystal Structure of the E. coli Hsp100 ClpB N-Terminal Domain journal March 2003
Structure and mechanism of the hexameric MecA–ClpC molecular machine journal March 2011
ClpS, a Substrate Modulator of the ClpAP Machine journal March 2002
Species-specific collaboration of heat shock proteins (Hsp) 70 and 100 in thermotolerance and protein disaggregation journal April 2011
The M-Domain Controls Hsp104 Protein Remodeling Activity in an Hsp70/Hsp40-Dependent Manner journal September 2010
M Domains Couple the ClpB Threading Motor with the DnaK Chaperone Activity journal January 2007
Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces journal November 2012
Heat shock protein (Hsp) 70 is an activator of the Hsp104 motor journal May 2013
CryoEM structure of Hsp104 and its mechanistic implication for protein disaggregation journal April 2010
Mechanistic Insights into Hsp104 Potentiation journal March 2016
N-Terminal Domain of Yeast Hsp104 Chaperone Is Dispensable for Thermotolerance and Prion Propagation but Necessary for Curing Prions by Hsp104 Overexpression journal April 2006
Potentiated Hsp104 Variants Antagonize Diverse Proteotoxic Misfolding Events journal January 2014
Potentiating Hsp104 activity via phosphomimetic mutations in the middle domain journal April 2018
Mining Disaggregase Sequence Space to Safely Counter TDP-43, FUS, and α-Synuclein Proteotoxicity journal August 2019
Engineering enhanced protein disaggregases for neurodegenerative disease journal March 2015
Disparate Mutations Confer Therapeutic Gain of Hsp104 Function journal October 2015
Cooperative kinetics of both Hsp104 ATPase domains and interdomain communication revealed by AAA sensor-1 mutants journal January 2002
Saccharomyces cerevisiae Hsp104 protein. Purification and characterization of ATP-induced structural changes. journal February 1994
Hspl04 is a highly conserved protein with two essential nucleotide-binding sites journal September 1991
Role of the chaperone protein Hsp104 in propagation of the yeast prion-like factor [psi+] journal May 1995
Analysis of the AAA sensor-2 motif in the C-terminal ATPase domain of Hsp104 with a site-specific fluorescent probe of nucleotide binding journal February 2002
Evidence for an Unfolding/Threading Mechanism for Protein Disaggregation by Saccharomyces cerevisiae Hsp104 journal May 2004
Peptide and Protein Binding in the Axial Channel of Hsp104: INSIGHTS INTO THE MECHANISM OF PROTEIN UNFOLDING journal August 2008
Substrate Binding to the Molecular Chaperone Hsp104 and Its Regulation by Nucleotides journal November 2005
Loops in the Central Channel of ClpA Chaperone Mediate Protein Binding, Unfolding, and Translocation journal July 2005
Stability of the two wings of the coiled-coil domain of ClpB chaperone is critical for its disaggregation activity journal June 2009
ATP Binding to Nucleotide Binding Domain (NBD)1 of the ClpB Chaperone Induces Motion of the Long Coiled-coil, Stabilizes the Hexamer, and Activates NBD2 journal July 2005
Dominant Gain-of-Function Mutations in Hsp104p Reveal Crucial Roles for the Middle Region journal May 2004
Atypical AAA+ Subunit Packing Creates an Expanded Cavity for Disaggregation by the Protein-Remodeling Factor Hsp104 journal December 2007
Roles of Individual Domains and Conserved Motifs of the AAA+ Chaperone ClpB in Oligomerization, ATP Hydrolysis, and Chaperone Activity journal March 2003
Regulation of the Hsp104 Middle Domain Activity Is Critical for Yeast Prion Propagation journal January 2014
A tightly regulated molecular toggle controls AAA+ disaggregase journal November 2012
Defining a Pathway of Communication from the C-Terminal Peptide Binding Domain to the N-Terminal ATPase Domain in a AAA Protein journal April 2002
Disruption of Ionic Interactions between the Nucleotide Binding Domain 1 (NBD1) and Middle (M) Domain in Hsp100 Disaggregase Unleashes Toxic Hyperactivity and Partial Independence from Hsp70 journal January 2013
Channel mutations in Hsp104 hexamer distinctively affect thermotolerance and prion-specific propagation: Prion-specific activity of Hsp104 journal March 2007
A Systematic Evaluation of the Function of the Protein-Remodeling Factor Hsp104 in [ PSI + ] Prion Propagation in S. cerevisiae by Comprehensive Chromosomal Mutations journal January 2007
Nucleocytoplasmic Trafficking of the Molecular Chaperone Hsp104 in Unstressed and Heat-Shocked Cells journal January 2008
Evolutionary Relationships and Structural Mechanisms of aaa+ Proteins journal June 2006
Amino Acid Substitutions in the C-terminal AAA + Module of Hsp104 Prevent Substrate Recognition by Disrupting Oligomerization and Cause High Temperature Inactivation journal June 2004
The ATPase Activity of Hsp104, Effects of Environmental Conditions and Mutations journal June 1998
Structural basis for intersubunit signaling in a protein disaggregating machine journal July 2012
HSP104 required for induced thermotolerance journal June 1990
Protein Modeling by E-mail journal July 1995
High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method journal January 2007
The Beamline X28C of the Center for Synchrotron Biosciences: a National Resource for Biomolecular Structure and Dynamics Experiments Using Synchrotron Footprinting journal April 2007
Synchrotron Protein Footprinting Supports Substrate Translocation by ClpA via ATP-Induced Movements of the D2 Loop journal August 2008
ExMS: Data Analysis for HX-MS Experiments journal September 2011
Integrated Algorithms for High-Throughput Examination of Covalently Labeled Biomolecules by Structural Mass Spectrometry journal October 2009
The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling journal November 2005
The SWISS-MODEL Repository and associated resources journal January 2009