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Title: OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions

Abstract

Summary Plant Phosphate Transporter 1 (PHT1) proteins, probably the only influx transporters for phosphate (Pi) uptake, are partially degraded on sufficient Pi levels to prevent excessive Pi accumulation. Therefore, the basal/constitutive expression level of PHT1 genes is vital for maintaining Pi uptake under Pi‐replete conditions. Rice ( Oryza sativa ) OsPHT1;1 is a unique gene as it is highly expressed and not responsive to Pi, however the mechanism for maintaining its basal/constitutive expression remains unknown. Using biochemical and genetic approaches, we identified and functionally characterised the transcription factors maintaining the basal/constitutive expression of OsPHT1;1 . OsWRKY21 and OsWRKY108 interact within the nucleus and both bind to the W‐box in the OsPHT1;1 promoter. Overexpression of OsWRKY21 or OsWRKY108 led to increased Pi accumulation, resulting from elevated expression of OsPHT1;1 . By contrast, oswrky21 oswrky108 double mutants showed decreased Pi accumulation and OsPHT1;1 expression in a Pi‐dependent manner. Moreover, similar to ospht1;1 mutants, plants expressing the OsWRKY21–SRDX fusion protein (a chimeric dominant suppressor) were impaired in Pi accumulation in Pi‐replete roots, accompanied by downregulation of OsPHT1;1 expression. Our findings demonstrated that rice WRKY transcription factors function redundantly to promote Pi uptake by activating OsPHT1;1 expression under Pi‐replete conditions, and represent a novelmore » pathway independent of the central Pi signalling system.« less

Authors:
 [1]; ORCiD logo [2];  [1];  [1];  [1];  [1];  [1];  [3];  [2];  [1]; ORCiD logo [2]
  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China
  2. State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China, MOA Key Laboratory of Plant Nutrition and Fertilisation in Lower‐Middle Reaches of the Yangtze River Nanjing 210095 China, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilisation Nanjing 210095 China
  3. State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China, MOA Key Laboratory of Plant Nutrition and Fertilisation in Lower‐Middle Reaches of the Yangtze River Nanjing 210095 China
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1671837
Alternate Identifier(s):
OSTI ID: 1781900
Resource Type:
Journal Article: Published Article
Journal Name:
New Phytologist
Additional Journal Information:
Journal Name: New Phytologist Journal Volume: 229 Journal Issue: 3; Journal ID: ISSN 0028-646X
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Zhang, Jun, Gu, Mian, Liang, Ruisuhua, Shi, Xinyu, Chen, Lingling, Hu, Xu, Wang, Shichao, Dai, Xiaoli, Qu, Hongye, Li, Huanhuan, and Xu, Guohua. OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions. United Kingdom: N. p., 2020. Web. doi:10.1111/nph.16931.
Zhang, Jun, Gu, Mian, Liang, Ruisuhua, Shi, Xinyu, Chen, Lingling, Hu, Xu, Wang, Shichao, Dai, Xiaoli, Qu, Hongye, Li, Huanhuan, & Xu, Guohua. OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions. United Kingdom. https://doi.org/10.1111/nph.16931
Zhang, Jun, Gu, Mian, Liang, Ruisuhua, Shi, Xinyu, Chen, Lingling, Hu, Xu, Wang, Shichao, Dai, Xiaoli, Qu, Hongye, Li, Huanhuan, and Xu, Guohua. 2020. "OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions". United Kingdom. https://doi.org/10.1111/nph.16931.
@article{osti_1671837,
title = {OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions},
author = {Zhang, Jun and Gu, Mian and Liang, Ruisuhua and Shi, Xinyu and Chen, Lingling and Hu, Xu and Wang, Shichao and Dai, Xiaoli and Qu, Hongye and Li, Huanhuan and Xu, Guohua},
abstractNote = {Summary Plant Phosphate Transporter 1 (PHT1) proteins, probably the only influx transporters for phosphate (Pi) uptake, are partially degraded on sufficient Pi levels to prevent excessive Pi accumulation. Therefore, the basal/constitutive expression level of PHT1 genes is vital for maintaining Pi uptake under Pi‐replete conditions. Rice ( Oryza sativa ) OsPHT1;1 is a unique gene as it is highly expressed and not responsive to Pi, however the mechanism for maintaining its basal/constitutive expression remains unknown. Using biochemical and genetic approaches, we identified and functionally characterised the transcription factors maintaining the basal/constitutive expression of OsPHT1;1 . OsWRKY21 and OsWRKY108 interact within the nucleus and both bind to the W‐box in the OsPHT1;1 promoter. Overexpression of OsWRKY21 or OsWRKY108 led to increased Pi accumulation, resulting from elevated expression of OsPHT1;1 . By contrast, oswrky21 oswrky108 double mutants showed decreased Pi accumulation and OsPHT1;1 expression in a Pi‐dependent manner. Moreover, similar to ospht1;1 mutants, plants expressing the OsWRKY21–SRDX fusion protein (a chimeric dominant suppressor) were impaired in Pi accumulation in Pi‐replete roots, accompanied by downregulation of OsPHT1;1 expression. Our findings demonstrated that rice WRKY transcription factors function redundantly to promote Pi uptake by activating OsPHT1;1 expression under Pi‐replete conditions, and represent a novel pathway independent of the central Pi signalling system.},
doi = {10.1111/nph.16931},
url = {https://www.osti.gov/biblio/1671837}, journal = {New Phytologist},
issn = {0028-646X},
number = 3,
volume = 229,
place = {United Kingdom},
year = {Sat Oct 10 00:00:00 EDT 2020},
month = {Sat Oct 10 00:00:00 EDT 2020}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1111/nph.16931

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Works referenced in this record:

Phosphorus saturation and mobilization in two typical Chinese greenhouse vegetable soils
journal, April 2017


pho2 , a Phosphate Overaccumulator, Is Caused by a Nonsense Mutation in a MicroRNA399 Target Gene
journal, May 2006


A plant’s diet, surviving in a variable nutrient environment
journal, April 2020


WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis
journal, February 2007


Negative Regulation of Phosphate Starvation-Induced Genes
journal, December 2001


Overexpression of OsMYB4P, an R2R3-type MYB transcriptional activator, increases phosphate acquisition in rice
journal, July 2014


Reducing the Genetic Redundancy of Arabidopsis PHOSPHATE TRANSPORTER1 Transporters to Study Phosphate Uptake and Signaling
journal, February 2015


Coordination between zinc and phosphate homeostasis involves the transcription factor PHR1, the phosphate exporter PHO1, and its homologue PHO1;H3 in Arabidopsis
journal, January 2014


OsWRKY28 Regulates Phosphate and Arsenate Accumulation, Root System Architecture and Fertility in Rice
journal, September 2018


WRKY transcription factors
journal, May 2010


The Rice CK2 Kinase Regulates Trafficking of Phosphate Transporters in Response to Phosphate Levels
journal, February 2015


High-CO 2 /Hypoxia-Responsive Transcription Factors DkERF24 and DkWRKY1 Interact and Activate DkPDC2 Promoter
journal, March 2019


The Phosphate Transporter Gene OsPht1;8 Is Involved in Phosphate Homeostasis in Rice
journal, April 2011


A Reevaluation of the Role of Arabidopsis NRT1.1 in High-Affinity Nitrate Transport
journal, October 2013


Node-Localized Transporters of Phosphorus Essential for Seed Development in Rice
journal, June 2020


Phosphate import in plants: focus on the PHT1 transporters
journal, January 2011


OsWRKY74 , a WRKY transcription factor, modulates tolerance to phosphate starvation in rice
journal, December 2015


Phosphate Starvation Responses and Gibberellic Acid Biosynthesis are Regulated by the MYB62 Transcription Factor in Arabidopsis
journal, January 2009


Phosphorus Dynamics: From Soil to Plant
journal, May 2011


Plastic transport systems of rice for mineral elements in response to diverse soil environmental changes
journal, December 2019


Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice ( Oryza sativa )
journal, September 2013


Regulation of rice responses to submergence by WRKY transcription factors
journal, September 2018


The WRKY Gene Family in Rice (Oryza sativa)
journal, June 2007


WRKY transcription factors: from DNA binding towards biological function
journal, October 2004


The WRKY6 Transcription Factor Modulates PHOSPHATE1 Expression in Response to Low Pi Stress in Arabidopsis
journal, November 2009


Regulation of miR399f Transcription by AtMYB2 Affects Phosphate Starvation Responses in Arabidopsis
journal, November 2012


A Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic Accumulation
journal, March 2007


Phosphate starvation induced OsPHR4 mediates Pi-signaling and homeostasis in rice
journal, November 2016


Phosphate transporters from the higher plant Arabidopsis thaliana.
journal, September 1996


OsPHR2 Is Involved in Phosphate-Starvation Signaling and Excessive Phosphate Accumulation in Shoots of Plants
journal, February 2008


OsPHT1;3 Mediates Uptake, Translocation, and Remobilization of Phosphate under Extremely Low Phosphate Regimes
journal, December 2018


Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2
journal, April 2013


The Diverse Roles of Rice PHO1 in Phosphate Transport: From Root to Node to Grain
journal, July 2020


Involvement of OsPht1;4 in phosphate acquisition and mobilization facilitates embryo development in rice
journal, May 2015


Phosphate Acquisition
journal, June 1999