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Title: A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level

Abstract

Abstract Current microfluidic methods for studying multicell strains (e.g., m-types) with multienvironments (e.g., n-types) require large numbers of inlets/outlets (m*n), a complicated procedure or expensive machinery. Here, we developed a novel two-layer-integrated method to combine different PDMS microchannel layers with different functions into one chip by a PDMS through-hole array, which improved the design of a PDMS-based microfluidic system. Using this method, we succeeded in converting 2 × m × n inlets/outlets into m + n inlets/outlets and reduced the time cost of loading processing (from m × n to m) of the device for studying multicell strains (e.g., m-types) in varied multitemporal environments (i.e., n-types). Using this device, the dynamic behavior of the cell-stress-response proteins was studied when the glucose concentration decreased from 2% to a series of lower concentrations. Our device could also be widely used in high-throughput studies of various stress responses, and the new concept of a multilayer-integrated fabrication method could greatly improve the design of PDMS-based microfluidic systems.

Authors:
 [1];  [2];  [1];  [1]
  1. The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, China
  2. Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, China
Publication Date:
Sponsoring Org.:
USDOE Office of Electricity (OE), Advanced Grid Research & Development. Power Systems Engineering Research
OSTI Identifier:
1668873
Grant/Contract Number:  
2020YFA0906900; SQ2018YFA090070-03
Resource Type:
Journal Article: Published Article
Journal Name:
Integrative Biology (Online)
Additional Journal Information:
Journal Name: Integrative Biology (Online) Journal Volume: 12 Journal Issue: 10; Journal ID: ISSN 1757-9708
Publisher:
Oxford University Press
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Chen, Kaiyue, Rong, Nan, Wang, Shujing, and Luo, Chunxiong. A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level. United Kingdom: N. p., 2020. Web. doi:10.1093/intbio/zyaa018.
Chen, Kaiyue, Rong, Nan, Wang, Shujing, & Luo, Chunxiong. A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level. United Kingdom. doi:10.1093/intbio/zyaa018.
Chen, Kaiyue, Rong, Nan, Wang, Shujing, and Luo, Chunxiong. Tue . "A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level". United Kingdom. doi:10.1093/intbio/zyaa018.
@article{osti_1668873,
title = {A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level},
author = {Chen, Kaiyue and Rong, Nan and Wang, Shujing and Luo, Chunxiong},
abstractNote = {Abstract Current microfluidic methods for studying multicell strains (e.g., m-types) with multienvironments (e.g., n-types) require large numbers of inlets/outlets (m*n), a complicated procedure or expensive machinery. Here, we developed a novel two-layer-integrated method to combine different PDMS microchannel layers with different functions into one chip by a PDMS through-hole array, which improved the design of a PDMS-based microfluidic system. Using this method, we succeeded in converting 2 × m × n inlets/outlets into m + n inlets/outlets and reduced the time cost of loading processing (from m × n to m) of the device for studying multicell strains (e.g., m-types) in varied multitemporal environments (i.e., n-types). Using this device, the dynamic behavior of the cell-stress-response proteins was studied when the glucose concentration decreased from 2% to a series of lower concentrations. Our device could also be widely used in high-throughput studies of various stress responses, and the new concept of a multilayer-integrated fabrication method could greatly improve the design of PDMS-based microfluidic systems.},
doi = {10.1093/intbio/zyaa018},
journal = {Integrative Biology (Online)},
issn = {1757-9708},
number = 10,
volume = 12,
place = {United Kingdom},
year = {2020},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1093/intbio/zyaa018

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