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Title: Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer

Abstract

Extracellular vesicles (EVs) are released by nearly all cell types as part of normal cell physiology, transporting biological cargo, including nucleic acids and proteins, across the cell membrane. In pathological states such as cancer, EV-derived cargo may mirror the altered state of the cell of origin. Exosomes are the smaller, 50–150 nanometer-sized EVs released from fusion of multivesicular endosomes with the plasma membrane. Exosomes play important roles in cell-cell communication and participate in multiple cancer processes, including invasion and metastasis. Therefore, proteomic analysis of exosomes is a promising approach to discover potential cancer biomarkers, even though it is still at an early stage. Herein, we critically review the advances in exosome isolation methods and their compatibility with mass spectrometry (MS)-based proteomic analysis, as well as studies of exosomes in pathogenesis and progression of prostate and bladder cancer, two common urologic cancers whose incidence rates continue to rise annually. As urological tumors, both urine and blood samples are feasible for noninvasive or minimally invasive analysis. A better understanding of the biological cargo and functions of exosomes via high-throughput proteomics will help provide new insights into complex alterations in cancer and provide potential therapeutic targets and personalized treatment for patients.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2];  [2]; ORCiD logo [1]; ORCiD logo [3]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. National Cancer Inst., Bethesda, MD (United States). Cancer Biomarkers Research Group
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Oregon Health and Science Univ., Portland, OR (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
OSTI Identifier:
1668329
Report Number(s):
PNNL-SA-154352
Journal ID: ISSN 2072-6694
Grant/Contract Number:  
AC05-76RL01830; U24CA210955; U01CA214116; R21CA223715; P41GM103493
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Cancers (Basel)
Additional Journal Information:
Journal Volume: 12; Journal Issue: 9; Journal ID: ISSN 2072-6694
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
exosome, proteomics, prostate cancer, bladder cancer, exosome isolation method

Citation Formats

Wang, Yi-Ting, Shi, Tujin, Srivastava, Sudhir, Kagan, Jacob, Liu, Tao, and Rodland, Karin D. Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer. United States: N. p., 2020. Web. doi:10.3390/cancers12092335.
Wang, Yi-Ting, Shi, Tujin, Srivastava, Sudhir, Kagan, Jacob, Liu, Tao, & Rodland, Karin D. Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer. United States. doi:10.3390/cancers12092335.
Wang, Yi-Ting, Shi, Tujin, Srivastava, Sudhir, Kagan, Jacob, Liu, Tao, and Rodland, Karin D. Wed . "Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer". United States. doi:10.3390/cancers12092335. https://www.osti.gov/servlets/purl/1668329.
@article{osti_1668329,
title = {Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer},
author = {Wang, Yi-Ting and Shi, Tujin and Srivastava, Sudhir and Kagan, Jacob and Liu, Tao and Rodland, Karin D.},
abstractNote = {Extracellular vesicles (EVs) are released by nearly all cell types as part of normal cell physiology, transporting biological cargo, including nucleic acids and proteins, across the cell membrane. In pathological states such as cancer, EV-derived cargo may mirror the altered state of the cell of origin. Exosomes are the smaller, 50–150 nanometer-sized EVs released from fusion of multivesicular endosomes with the plasma membrane. Exosomes play important roles in cell-cell communication and participate in multiple cancer processes, including invasion and metastasis. Therefore, proteomic analysis of exosomes is a promising approach to discover potential cancer biomarkers, even though it is still at an early stage. Herein, we critically review the advances in exosome isolation methods and their compatibility with mass spectrometry (MS)-based proteomic analysis, as well as studies of exosomes in pathogenesis and progression of prostate and bladder cancer, two common urologic cancers whose incidence rates continue to rise annually. As urological tumors, both urine and blood samples are feasible for noninvasive or minimally invasive analysis. A better understanding of the biological cargo and functions of exosomes via high-throughput proteomics will help provide new insights into complex alterations in cancer and provide potential therapeutic targets and personalized treatment for patients.},
doi = {10.3390/cancers12092335},
journal = {Cancers (Basel)},
issn = {2072-6694},
number = 9,
volume = 12,
place = {United States},
year = {2020},
month = {8}
}

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