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Title: Genetic identification of 91 poplar cultivars based on SSR markers

Abstract

Accurate genetic identification and relationship analysis of poplar cultivars is necessary to establish commercial poplar plantations and select suitable breeding strategies. In this study, 91 poplar cultivars belonging to four sections (Aigeiros, Tacamahaca, Populus and Turanga) and inter/intra-sectional hybrids were genotyped using 18 polymorphic simple sequence repeat (SSR) markers. In total, 222 alleles were amplified with an average of 12.3 alleles per marker. The mean polymorphic information content and power of discrimination were 0.706 and 0.813, respectively. Five SSR markers (ORPM_103, ORPM_247, GCPM_1048, GCPM_1255 and LG_X_19) constituted a core fingerprint and were sufficient to identify all the tested cultivars. With some notable exceptions, cultivars of the same species generally clustered together in cluster (UPGMA) and ordination (PCO) analyses. Flow cytometry indicated that 11 poplar cultivars were triploid. Among these, seven had three alleles at some loci, suggesting that SSR markers could indicate the ploidy level to some extent. This study provides useful genetic information for the identification and protection of poplar cultivars in China and offers a guideline for the selection of poplar crossing parents based on ploidy level and genetic relationships.

Authors:
 [1];  [1];  [1];  [1];  [1]; ORCiD logo [2];  [3]; ORCiD logo [3]
  1. Chinese Academy of Forestry, Beijing (China)
  2. Chinese Academy of Forestry, Beijing (China); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Chinese Academy of Forestry, Beijing (China); Nanjing Forestry Univ. (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1665979
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nordic Journal of Botany
Additional Journal Information:
Journal Volume: 38; Journal Issue: 6; Journal ID: ISSN 0107-055X
Country of Publication:
United States
Language:
English
Subject:
fingerprint; flow cytometry; Populus triploid; UPGMA analysis

Citation Formats

Sun, Pei, Jia, Huixia, Yue, Zhiqiang, Li, Jinhua, Li, Jianbo, Zhang, Jin, Lu, Mengzhu, and Hu, Jianjun. Genetic identification of 91 poplar cultivars based on SSR markers. United States: N. p., 2020. Web. doi:10.1111/njb.02504.
Sun, Pei, Jia, Huixia, Yue, Zhiqiang, Li, Jinhua, Li, Jianbo, Zhang, Jin, Lu, Mengzhu, & Hu, Jianjun. Genetic identification of 91 poplar cultivars based on SSR markers. United States. doi:10.1111/njb.02504.
Sun, Pei, Jia, Huixia, Yue, Zhiqiang, Li, Jinhua, Li, Jianbo, Zhang, Jin, Lu, Mengzhu, and Hu, Jianjun. Tue . "Genetic identification of 91 poplar cultivars based on SSR markers". United States. doi:10.1111/njb.02504.
@article{osti_1665979,
title = {Genetic identification of 91 poplar cultivars based on SSR markers},
author = {Sun, Pei and Jia, Huixia and Yue, Zhiqiang and Li, Jinhua and Li, Jianbo and Zhang, Jin and Lu, Mengzhu and Hu, Jianjun},
abstractNote = {Accurate genetic identification and relationship analysis of poplar cultivars is necessary to establish commercial poplar plantations and select suitable breeding strategies. In this study, 91 poplar cultivars belonging to four sections (Aigeiros, Tacamahaca, Populus and Turanga) and inter/intra-sectional hybrids were genotyped using 18 polymorphic simple sequence repeat (SSR) markers. In total, 222 alleles were amplified with an average of 12.3 alleles per marker. The mean polymorphic information content and power of discrimination were 0.706 and 0.813, respectively. Five SSR markers (ORPM_103, ORPM_247, GCPM_1048, GCPM_1255 and LG_X_19) constituted a core fingerprint and were sufficient to identify all the tested cultivars. With some notable exceptions, cultivars of the same species generally clustered together in cluster (UPGMA) and ordination (PCO) analyses. Flow cytometry indicated that 11 poplar cultivars were triploid. Among these, seven had three alleles at some loci, suggesting that SSR markers could indicate the ploidy level to some extent. This study provides useful genetic information for the identification and protection of poplar cultivars in China and offers a guideline for the selection of poplar crossing parents based on ploidy level and genetic relationships.},
doi = {10.1111/njb.02504},
journal = {Nordic Journal of Botany},
issn = {0107-055X},
number = 6,
volume = 38,
place = {United States},
year = {2020},
month = {6}
}

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