Arabidopsis C‐terminal binding protein ANGUSTIFOLIA modulates transcriptional co‐regulation of MYB46 and WRKY33

Xie, Meng; Zhang, Jin; Yao, Tao; Bryan, Anthony C.; Pu, Yunqiao; Labbé, Jessy; Pelletier, Dale A.; Engle, Nancy; Morrell‐Falvey, Jennifer L.; Schmutz, Jeremy; Ragauskas, Arthur J.; Tschaplinski, Timothy J.; Chen, Feng; Tuskan, Gerald A.; Muchero, Wellington; Chen, Jin‐Gui

doi:10.1111/nph.16826

Title: Arabidopsis C‐terminal binding protein ANGUSTIFOLIA modulates transcriptional co‐regulation of MYB46 and WRKY33

Journal Article · Wed Aug 19 00:00:00 EDT 2020 · New Phytologist

DOI:https://doi.org/10.1111/nph.16826· OSTI ID:1651230

^[1];

^[2]; Yao, Tao ^[3]; Bryan, Anthony C. ^[4]; Pu, Yunqiao ^[2];

^[2]; Pelletier, Dale A. ^[5]; Engle, Nancy ^[2]; Morrell‐Falvey, Jennifer L. ^[5]; Schmutz, Jeremy ^[6]; Ragauskas, Arthur J. ^[7]; Tschaplinski, Timothy J. ^[2]; Chen, Feng ^[8]; Tuskan, Gerald A. ^[2]; Muchero, Wellington ^[2];

^[2]

Biosciences Division Oak Ridge National Laboratory Oak Ridge TN37831USA, BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge TN37831USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN37831USA, Department of Plant Sciences University of Tennessee Knoxville TN37996USA, Biology Department Brookhaven National Laboratory Upton NY11973USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN37831USA, BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge TN37831USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN37831USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN37831USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN37831USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN37831USA, BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge TN37831USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN37831USA
US Department of Energy Joint Genome Institute Berkeley CA94720USA, HudsonAlpha Institute for Biotechnology Huntsville AL35806USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN37831USA, BioEnergy Science Center Oak Ridge National Laboratory Oak Ridge TN37831USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN37831USA, UT‐ORNL Joint Institute for Biological Science Oak Ridge National Laboratory Oak Ridge TN37831USA, Department of Chemical and Biomolecular Engineering &, Department of Forestry, Wildlife, and Fisheries University of Tennessee Knoxville TN37996USA
Department of Plant Sciences University of Tennessee Knoxville TN37996USA

The apparent antagonism between Salicylic Acid (SA) and Jasmonic acid (JA)/Ethylene (ET) signaling resulting in tradeoffs between defense against (hemi)biotrophic and necrotrophic pathogens has been widely described across multiple plant species. However, the underlying mechanism remains to be fully established.The molecular and cellular function of ANGUSTIFOLIA (AN) were characterized, and its roles in regulating pathogenic response were studied in Arabidopsis.We demonstrated that AN, a plant homolog of mammalian C-TERMINAL BINDING PROTEIN (CtBP), antagonistically regulates plant resistance to the hemibiotrophic pathogen Pseudomonas syringae and the necrotrophic pathogen Botrytis cinerea . Consistent with the phenotypic observations, transcription of genes involved in SA and JA/ET pathways was antagonistically regulated by AN. By interacting with another nuclear protein TYROSYL-DNA PHOSPHODIESTERASE1 (TDP1), AN imposes transcriptional repression on MYB46 , encoding a transcriptional activator of PHENYLALANINE AMMONIA-LYASE (PAL ) genes which are required for SA biosynthesis, while releasing the TDP1-imposed transcriptional repression on WRKY33 , a master regulator of the JA/ET signaling pathway.These findings demonstrate that the transcriptional co-regulation of MYB46 and WRKY33 by AN mediates the coordination of SA and JA/ET pathways to optimize defenses against (hemi)biotrophic and necrotrophic pathogens.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; SC0012704; AC02-05CH11231

OSTI ID:: 1651230

Alternate ID(s):: OSTI ID: 1649652; OSTI ID: 1649989; OSTI ID: 1651232

Report Number(s):: BNL-216284-2020-JAAM

Journal Information:: New Phytologist, Journal Name: New Phytologist; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Title: Arabidopsis C‐terminal binding protein ANGUSTIFOLIA modulates transcriptional co‐regulation of MYB46 and WRKY33

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