Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus

Cleare, Levi G.; Zamith, Daniel; Heyman, Heino M.; Couvillion, Sneha P.; Nimrichter, Leonardo; Rodrigues, Marcio L.; Nakayasu, Ernesto S.; Nosanchuk, Joshua D.

doi:10.1111/cmi.13217

Title: Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus

Journal Article · Mon Jun 22 00:00:00 EDT 2020 · Cellular Microbiology

DOI:https://doi.org/10.1111/cmi.13217· OSTI ID:1646823

^[1]; Zamith, Daniel ^[1]; Heyman, Heino M. ^[2]; Couvillion, Sneha P. ^[3];

^[4]; Rodrigues, Marcio L. ^[5]; Nakayasu, Ernesto S. ^[3]; Nosanchuk, Joshua D. ^[1]

Albert Einstein College of Medicine, Bronx, NY (United States). Dept. of Medicine and Dept.of Microbiology and Immunology
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division; Albert Einstein College of Medicine, Bronx, NY (United States). Dept. of Medicine and Dept.of Microbiology and Immunology
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
Univ. Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (Brazil). Inst. de Microbiologia Paulo de Góes
Univ. Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (Brazil). Inst. de Microbiologia Paulo de Góes; Fundação Oswaldo Cruz (Fiocruz), Curitiba (Brazil). Inst. Carlos Chagas

Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. H. capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. H. capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In our research, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação Oswaldo Cruz (Fiocruz)

Grant/Contract Number:: AC05-76RL01830; R01AI052733; R21AI124797; 405520/2018-2; 440015/2018-9; 301304/2017-3; 311179/2017-7; 408711/2017-7; VPPCB-007-FIO-18-2- 57; VPPIS-001-FIO-18-66

OSTI ID:: 1646823

Report Number(s):: PNNL-SA-151908

Journal Information:: Cellular Microbiology, Vol. 22, Issue 9; ISSN 1462-5814

Publisher:: Wiley Hindawi PartnershipCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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Title: Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus

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