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Title: Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass

Abstract

Lignocellulosic materials are plant-derived feedstocks, such as crop residues (e.g., corn stover, rice straw, and sugar cane bagasse) and purpose-grown energy crops (e.g., miscanthus, and switchgrass) that are available in large quantities to produce biofuels, biochemicals, and animal feed. Plant polysaccharides (i.e., cellulose, hemicellulose, and pectin) embedded within cell walls are highly recalcitrant towards conversion into useful products. Ammonia fiber expansion (AFEX) is a thermochemical pretreatment that increases accessibility of polysaccharides to enzymes for hydrolysis into fermentable sugars. These released sugars can be converted into fuels and chemicals in a biorefinery. Here, we describe a laboratory-scale batch AFEX process to produce pretreated biomass on the gram-scale without any ammonia recycling. The laboratory-scale process can be used to identify optimal pretreatment conditions (e.g., ammonia loading, water loading, biomass loading, temperature, pressure, residence time, etc.) and generates sufficient quantities of pretreated samples for detailed physicochemical characterization and enzymatic/microbial analysis. The yield of fermentable sugars from enzymatic hydrolysis of corn stover pretreated using the laboratory-scale AFEX process is comparable to pilot-scale AFEX process under similar pretreatment conditions. This paper is intended to provide a detailed standard operating procedure for the safe and consistent operation of laboratory-scale reactors for performing AFEX pretreatment of lignocellulosicmore » biomass.« less

Authors:
 [1];  [1];  [1];  [2];  [2];  [2];  [2];  [3];  [3];  [3];  [4];  [5];  [5];  [5];  [5]
  1. Rutgers Univ., Piscataway, NJ (United States)
  2. Michigan Biotechnology Inst. (MBI), Lansing, MI (United States)
  3. Michigan State Univ., East Lansing, MI (United States)
  4. Univ. of Houston, TX (United States)
  5. Michigan Technological Univ., Houghton, MI (United States)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1637459
Grant/Contract Number:  
SC0018409; FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Visualized Experiments
Additional Journal Information:
Journal Issue: 158; Journal ID: ISSN 1940-087X
Publisher:
MyJoVE Corp.
Country of Publication:
United States
Language:
English
Subject:
environmental sciences; Issue 158; Ammonia Fiber Expansion (AFEX) pretreatment; lignocellulosic biomass; cellulosic biofuels; animal feed; cellulases; enzymatic hydrolysis; biorefinery

Citation Formats

Chundawat, Shishir P. S., Pal, Ramendra K., Zhao, Chao, Campbell, Timothy, Teymouri, Farzaneh, Videto, Josh, Nielson, Chandra, Wieferich, Bradley, Sousa, Leonardo, Dale, Bruce E., Balan, Venkatesh, Chipkar, Sarvada, Aguado, Jacob, Burke, Emily, and Ong, Rebecca G. Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass. United States: N. p., 2020. Web. doi:10.3791/57488.
Chundawat, Shishir P. S., Pal, Ramendra K., Zhao, Chao, Campbell, Timothy, Teymouri, Farzaneh, Videto, Josh, Nielson, Chandra, Wieferich, Bradley, Sousa, Leonardo, Dale, Bruce E., Balan, Venkatesh, Chipkar, Sarvada, Aguado, Jacob, Burke, Emily, & Ong, Rebecca G. Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass. United States. doi:10.3791/57488.
Chundawat, Shishir P. S., Pal, Ramendra K., Zhao, Chao, Campbell, Timothy, Teymouri, Farzaneh, Videto, Josh, Nielson, Chandra, Wieferich, Bradley, Sousa, Leonardo, Dale, Bruce E., Balan, Venkatesh, Chipkar, Sarvada, Aguado, Jacob, Burke, Emily, and Ong, Rebecca G. Sat . "Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass". United States. doi:10.3791/57488. https://www.osti.gov/servlets/purl/1637459.
@article{osti_1637459,
title = {Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass},
author = {Chundawat, Shishir P. S. and Pal, Ramendra K. and Zhao, Chao and Campbell, Timothy and Teymouri, Farzaneh and Videto, Josh and Nielson, Chandra and Wieferich, Bradley and Sousa, Leonardo and Dale, Bruce E. and Balan, Venkatesh and Chipkar, Sarvada and Aguado, Jacob and Burke, Emily and Ong, Rebecca G.},
abstractNote = {Lignocellulosic materials are plant-derived feedstocks, such as crop residues (e.g., corn stover, rice straw, and sugar cane bagasse) and purpose-grown energy crops (e.g., miscanthus, and switchgrass) that are available in large quantities to produce biofuels, biochemicals, and animal feed. Plant polysaccharides (i.e., cellulose, hemicellulose, and pectin) embedded within cell walls are highly recalcitrant towards conversion into useful products. Ammonia fiber expansion (AFEX) is a thermochemical pretreatment that increases accessibility of polysaccharides to enzymes for hydrolysis into fermentable sugars. These released sugars can be converted into fuels and chemicals in a biorefinery. Here, we describe a laboratory-scale batch AFEX process to produce pretreated biomass on the gram-scale without any ammonia recycling. The laboratory-scale process can be used to identify optimal pretreatment conditions (e.g., ammonia loading, water loading, biomass loading, temperature, pressure, residence time, etc.) and generates sufficient quantities of pretreated samples for detailed physicochemical characterization and enzymatic/microbial analysis. The yield of fermentable sugars from enzymatic hydrolysis of corn stover pretreated using the laboratory-scale AFEX process is comparable to pilot-scale AFEX process under similar pretreatment conditions. This paper is intended to provide a detailed standard operating procedure for the safe and consistent operation of laboratory-scale reactors for performing AFEX pretreatment of lignocellulosic biomass.},
doi = {10.3791/57488},
journal = {Journal of Visualized Experiments},
issn = {1940-087X},
number = 158,
volume = ,
place = {United States},
year = {2020},
month = {4}
}

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