Analysis of periplasmic sensor domains from Anaeromyxobacter dehalogenans 2 CP ‐C: Structure of one sensor domain from a histidine kinase and another from a chemotaxis protein

Pokkuluri, P. Raj; Dwulit‐Smith, Jeff; Duke, Norma E.; Wilton, Rosemarie; Mack, Jamey C.; Bearden, Jessica; Rakowski, Ella; Babnigg, Gyorgy; Szurmant, Hendrik; Joachimiak, Andrzej; Schiffer, Marianne

doi:10.1002/mbo3.112

Title: Analysis of periplasmic sensor domains from Anaeromyxobacter dehalogenans 2 CP ‐C: Structure of one sensor domain from a histidine kinase and another from a chemotaxis protein

Journal Article · Tue Jul 30 00:00:00 EDT 2013 · MicrobiologyOpen

DOI:https://doi.org/10.1002/mbo3.112· OSTI ID:1634844

Pokkuluri, P. Raj ^[1]; Dwulit‐Smith, Jeff ^[1]; Duke, Norma E. ^[2]; Wilton, Rosemarie ^[1]; Mack, Jamey C. ^[3]; Bearden, Jessica ^[3]; Rakowski, Ella ^[3]; Babnigg, Gyorgy ^[4]; Szurmant, Hendrik ^[5]; Joachimiak, Andrzej ^[6]; Schiffer, Marianne ^[4]

Biosciences Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439
Biosciences Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439, Structural Biology Center Biosciences division Argonne National Laboratory Lemont Illinois 60439
The Midwest Center for Structural Genomics Biosciences division Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439
Biosciences Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439, The Midwest Center for Structural Genomics Biosciences division Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439
Department of Molecular and Experimental Medicine The Scripps Research Institute 10550 N Torrey Pines Rd La Jolla California 92037
Biosciences Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439, Structural Biology Center Biosciences division Argonne National Laboratory Lemont Illinois 60439, The Midwest Center for Structural Genomics Biosciences division Argonne National Laboratory 9700 South Cass Avenue Lemont Illinois 60439

Abstract Anaeromyxobacter dehalogenans is a δ‐proteobacterium found in diverse soils and sediments. It is of interest in bioremediation efforts due to its dechlorination and metal‐reducing capabilities. To gain an understanding on A. dehalogenans' abilities to adapt to diverse environments we analyzed its signal transduction proteins. The A. dehalogenans genome codes for a large number of sensor histidine kinases ( HK ) and methyl‐accepting chemotaxis proteins ( MCP ); among these 23 HK and 11 MCP proteins have a sensor domain in the periplasm. These proteins most likely contribute to adaptation to the organism's surroundings. We predicted their three‐dimensional folds and determined the structures of two of the periplasmic sensor domains by X‐ray diffraction. Most of the domains are predicted to have either PAS ‐like or helical bundle structures, with two predicted to have solute‐binding protein fold, and another predicted to have a 6‐phosphogluconolactonase like fold. Atomic structures of two sensor domains confirmed the respective fold predictions. The Adeh_2942 sensor ( HK ) was found to have a helical bundle structure, and the Adeh_3718 sensor ( MCP ) has a PAS ‐like structure. Interestingly, the Adeh_3718 sensor has an acetate moiety bound in a binding site typical for PAS ‐like domains. Future work is needed to determine whether Adeh_3718 is involved in acetate sensing by A. dehalogenans .

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Sponsoring Organization:: USDOE

OSTI ID:: 1634844

Journal Information:: MicrobiologyOpen, Journal Name: MicrobiologyOpen Vol. 2 Journal Issue: 5; ISSN 2045-8827

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 7 works

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