Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440

Salvachua, Davinia; Werner, Allison; Pardo, Isabel; Michalska, Karolina; Black, Brenna A.; Donohoe, Bryon; Haugen, Stefan J.; Katahira, Rui; Notonier, Sandra; Ramirez, Kelsey; Amore, Antonella; Purvine, Samuel O.; Zink, Erika M.; Abraham, Paul; Giannone, Richard J.; Poudel, Suresh; Laible, Philip; Hettich, Robert L.; Beckham, Gregg T.

doi:10.1073/pnas.1921073117

Title: Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440

Journal Article · Tue Apr 28 00:00:00 EDT 2020 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1921073117· OSTI ID:1633770

Salvachua, Davinia ^[1]; Werner, Allison ^[2]; Pardo, Isabel ^[1]; Michalska, Karolina ^[3]; Black, Brenna A. ^[1]; Donohoe, Bryon ^[2]; Haugen, Stefan J. ^[1]; Katahira, Rui ^[1]; Notonier, Sandra ^[4]; Ramirez, Kelsey ^[1]; Amore, Antonella ^[1];

^[5];

^[5]; Abraham, Paul ^[6]; Giannone, Richard J. ^[6]; Poudel, Suresh ^[6]; Laible, Philip ^[3]; Hettich, Robert L. ^[7]; Beckham, Gregg T. ^[1]

National Renewable Energy Laboratory
NATIONAL RENEWABLE ENERGY LABORATORY
Argonne National Laboratory
National Renewable Engergy Laboratory
BATTELLE (PACIFIC NW LAB)
Oak Ridge National Laboratory
Oak Ridge National Laboratory (ORNL)

Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in nature is typically attributed to fungi, growing evidence suggests that bacteria also catabolize this complex biopolymer. However, the spatiotemporal mechanisms for lignin catabolism remain unclear. Improved understanding of this biological process would aid in our collective knowledge of both carbon cycling and microbial strategies to valorize lignin to value-added compounds. Here, we examine lignin modifications and the exoproteome of three aromatic catabolic bacteria: Pseudomonas putida KT2440, Rhodoccocus jostii RHA1, and Amycolatopsis sp. ATCC 39116. P. putida cultivation on lignin-rich media is characterized by an abundant exoproteome that is dynamically and selectively packaged into outer membrane vesicles (OMVs). Interestingly, many enzymes known to exhibit activity towards lignin-derived aromatic compounds are enriched in OMVs from early to late stationary phase, corresponding to the shift from bioavailable carbon to oligomeric lignin as a carbon source. In vivo and in vitro experiments demonstrate that enzymes contained in the OMVs are active and catabolize aromatic compounds. Taken together, this work supports OMV-mediated catabolism of lignin-derived aromatic compounds as an extracellular strategy for nutrient acquisition by soil bacteria.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1633770

Report Number(s):: PNNL-SA-149636

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 117, Issue 17

Country of Publication:: United States

Language:: English

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