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Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium-Mediated Method

Journal Article · · Current Protocols in Plant Biology
DOI:https://doi.org/10.1002/cppb.20077· OSTI ID:1633419
 [1];  [2];  [3];  [3];  [2]
  1. Univ. of Missouri, Columbia, MO (United States); Vietnam Academy of Science and Technology, Hanoi (Vietnam)
  2. Univ. of Missouri, Columbia, MO (United States)
  3. Univ. of Rhode Island, Kingston, RI (United States)
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Genetic transformation via Agrobacterium-mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In this article, we describe a sorghum transformation procedure in sufficient detail using a public genotype, P898012. This system utilizes a standard binary transgenic vector carrying the bar gene as a selectable marker and immature embryos as starting explants. Glufosinate is employed as the selective agent during callus and shoot induction. Further, this procedure is relatively rapid, efficient, highly reproducible, and should be applicable for many other sorghum genotypes

Research Organization:
Univ. of Rhode Island, Kingston, RI (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
DOE Contract Number:
SC0018277
OSTI ID:
1633419
Journal Information:
Current Protocols in Plant Biology, Vol. 3, Issue 4; ISSN 2379-8068
Publisher:
Wiley
Country of Publication:
United States
Language:
English

References (6)

The investigation of optimal bombardment parameters for transient and stable transgene expression in Sorghum May 2001
Development of marker-free transgenic sorghum [Sorghum bicolor (L.) Moench] using standard binary vectors with bar as a selectable marker August 2009
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency January 2004
Transgenic sorghum plants via microprojectile bombardment December 1993
Efficient, reproducible Agrobacterium-mediated transformation of sorghum using heat treatment of immature embryos December 2008
Rapid and efficient Agrobacterium-mediated transformation of sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker June 2016

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Related Subjects

Agrobacterium-mediated
genetic transformation
P898012
sorghum
standard binary vector